Natural Cysticercus fasciolaris infection in rodents from a rural area in Yucatan, Mexico

Medina-Pinto, Rodrigo; Torres–Castro, Marco; Medina-Pinto, Rolando A.; Bolio-González, Manuel Emilio; Rodríguez-Vivas, Róger Iván

doi:10.22201/fmvz.24486760e.2019.2.590

Cited by 7 publications

(10 citation statements)

References 26 publications

(24 reference statements)

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“…We also highlight the high ea species richness recorded in R. rattus, where 10 of the 14 species identi ed in this rodent correspond to the ea species identi ed on native rodents. This rodent was mainly captured in urban areas; however, we also found it in rural and natural areas, this occurs mainly because these rodents have an omnivore diet and plasticity in their behavior, characteristics that allow them to inhabit a great diversity of environments, adapting successfully to urban, rural and wild environments [30,31]. Rickettsia-positive eas parasitizing R. rattus in these three areas indicate that this species could play a key role in spreading the disease from wild to urban environments [16,32].…”

Section: Discussionmentioning

confidence: 85%

Molecular detection of Rickettsia in fleas from micromammals in Chile

Moreno

Carniglia²,

Schmeisser

et al. 2020

Preprint

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Background: Rickettsial diseases are considered important in public health due to their dispersal capacity determined by the particular characteristics of their reservoirs and/or vectors. Among the latter, fleas play an important role, since the vast majority of species parasitize wild and invasive rodents, so their detection is relevant to be able to monitor potential emerging diseases. The aim of this study was to detect, characterize, and compare Rickettsia spp. from the fleas of micromammals in areas with different human population densities in Chile. Methods: The presence of Rickettsia spp. was evaluated by standard polymerase chain reaction (PCR) and sequencing in 1315 fleas collected from 1512 micromammals in 29 locations, with different human population densities in Chile. A generalized linear model (GLM) was used to identify the variables that may explain Rickettsia prevalence in fleas. Results: DNA of Rickettsia spp. was identified in 13.2% (174 of 1315) of fleas tested. Fifteen flea species were found to be Rickettsia-positive. The prevalence of Rickettsia spp. was higher in winter, semi-arid region and natural areas, and the infection levels in fleas varied between species of flea. The prevalence of Rickettsia among flea species ranged between 0–35.1%. Areas of lower human density showed the highest prevalence of Rickettsia. The phylogenetic tree showed two well-differentiated clades with Rickettsia bellii positioned as basal in one clade. The second clade was subdivided into two subclades of species related to Rickettsia of the spotted fever group. Conclusions: To our knowledge, this is the first report of the occurrence and molecular characterization of Rickettsia spp. in 15 flea species of micromammals in Chile. In this study, fleas were detected carrying Rickettsia DNA with zoonotic potential, mainly in villages and natural areas of Chile. Considering that there are differences in the prevalence of Rickettsia in fleas associated with different factors, more investigations are needed to further understand the ecology of Rickettsia in fleas and their implications for human health.

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Section: Discussionmentioning

confidence: 85%

Molecular detection of Rickettsia in fleas from micromammals in Chile

Moreno

Carniglia²,

Schmeisser

et al. 2020

Preprint

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show abstract

“…We detected infection with H. taeniaeformis at a prevalence of 20.9%, which is consistent with the previous study in Rattus spp. in the Assiut Governorate [29], but higher than the prevalence of 7.8% reported in the same host in Yucatan Mexico [9] and of 7.4% reported in Rattus spp. in Iran [32].…”

Section: Discussionmentioning

confidence: 99%

“…Available at www.veterinaryworld.org/Vol.14/August-2021/24.pdf Cysticercus fasciolaris, Hydatigera fasciolaris, Strobilocercus fasciolaris, or T. crassicolis), which is found worldwide and primarily infects the small intestines of felines and canines following the ingestion of infected rodent livers [9]. H. taeniaeformis is a potential zoonotic parasite.…”

Section: Introductionmentioning

confidence: 99%

A parasitological survey of zoonotic cestodes carried by house rats in Aswan, Egypt, reveals cryptic diversity at the molecular level

et al. 2021

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Background and Aim: Some rat cestodes are zoonotic and are capable of parasitizing humans and animals, raising serious concerns regarding human and veterinary health. The study aimed to determine the prevalence and risk factors for cestodes in Egyptian house rats and to characterize the cestodes molecularly. Materials and Methods: The current survey examined 115 house rats (Rattus rattus) in two cities (Edfu and Aswan) in Egypt's Aswan Governorate for cestode infection using integrated molecular approaches (polymerase chain reaction, sequencing, and phylogenetic analysis) and morphological/morphometrical approaches. Results: The cestodes identified in this study exhibited the typical morphological characteristics of Hymenolepis diminuta (Rudolphi, 1819), Hymenolepis nana (Siebold, 1852) (from rat intestine), and Hydatigera taeniaeformis (from rat liver). The species prevalence rates from these three studies were reported to be 8.7%, 10.4%, and 20.9%, respectively. The ribosomal DNA (ITS1, 18S, and complete ITS) sequences revealed that the hymenolepid sequences were highly distinct but were related to other sequences in the GenBank database, with some sequences showing high similarities to those of H. nana and H. diminuta. In addition, the H. taeniaeformis sequences (ITS2 and mitochondrial cytochrome c oxidase subunit 1 [mtCOX1]) obtained in this study were highly similar to some Taenia taeniaeformis GenBank sequences. The constructed phylogram revealed that the hymenolepidid tapeworms examined in this study were classified into four major branches (the majority of which were hybrids of the two species) and belonged to the genus Hymenolepis. In addition, the phylogram of H. taeniaeformis assigned this species to T. taeniaeformis. Conclusion: When typical hymenolepid morphology is combined with molecular and phylogenetic divergence, it may indicate the existence of possible cryptic species. In addition, on the basis of the phylogenetic analysis, genetic diversity within T. taeniaeformis may exist as determined by comparing the metacestode mtCOX1 sequences. The current study presents the prevalence values of zoonotic cestodes and contributes to the body of knowledge, including identification keys and the use of molecular tools for species confirmation.

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“…We also highlight the high flea species richness recorded in R. rattus , where 10 of the 14 species identified in this rodent correspond to the flea species identified on native rodents. This rodent was mainly captured in urban areas; however, we also found it in rural and natural areas, this occurs mainly because these rodents have an omnivore diet and plasticity in their behavior, characteristics that allow them to inhabit a great diversity of environments, adapting successfully to urban, rural and wild environments [ 30 , 31 ]. Rickettsia- positive fleas parasitizing R. rattus in these three areas indicate that this species could play a key role in spreading the disease from wild to urban environments [ 16 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of Rickettsia in fleas from micromammals in Chile

et al. 2020

View full text Add to dashboard Cite

Background Rickettsial diseases are considered important in public health due to their dispersal capacity determined by the particular characteristics of their reservoirs and/or vectors. Among the latter, fleas play an important role, since the vast majority of species parasitize wild and invasive rodents, so their detection is relevant to be able to monitor potential emerging diseases. The aim of this study was to detect, characterize, and compare Rickettsia spp. from the fleas of micromammals in areas with different human population densities in Chile. Methods The presence of Rickettsia spp. was evaluated by standard polymerase chain reaction (PCR) and sequencing in 1315 fleas collected from 1512 micromammals in 29 locations, with different human population densities in Chile. A generalized linear model (GLM) was used to identify the variables that may explain Rickettsia prevalence in fleas. Results DNA of Rickettsia spp. was identified in 13.2% (174 of 1315) of fleas tested. Fifteen flea species were found to be Rickettsia-positive. The prevalence of Rickettsia spp. was higher in winter, semi-arid region and natural areas, and the infection levels in fleas varied between species of flea. The prevalence of Rickettsia among flea species ranged between 0–35.1%. Areas of lower human density showed the highest prevalence of Rickettsia. The phylogenetic tree showed two well-differentiated clades with Rickettsia bellii positioned as basal in one clade. The second clade was subdivided into two subclades of species related to Rickettsia of the spotted fever group. Conclusions To our knowledge, this is the first report of the occurrence and molecular characterization of Rickettsia spp. in 15 flea species of micromammals in Chile. In this study, fleas were detected carrying Rickettsia DNA with zoonotic potential, mainly in villages and natural areas of Chile. Considering that there are differences in the prevalence of Rickettsia in fleas associated with different factors, more investigations are needed to further understand the ecology of Rickettsia in fleas and their implications for human health.

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Natural Cysticercus fasciolaris infection in rodents from a rural area in Yucatan, Mexico

Cited by 7 publications

References 26 publications

Molecular detection of Rickettsia in fleas from micromammals in Chile

Molecular detection of Rickettsia in fleas from micromammals in Chile

A parasitological survey of zoonotic cestodes carried by house rats in Aswan, Egypt, reveals cryptic diversity at the molecular level

Molecular detection of Rickettsia in fleas from micromammals in Chile

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