Natural competence in <i><scp>V</scp>ibrio cholerae</i> is controlled by a nucleoside scavenging response that requires <scp>CytR</scp>‐dependent anti‐activation

Antonova, Elena S.; Bernardy, Eryn E.; Hammer, Brian K.

doi:10.1111/mmi.12054

Cited by 51 publications

(90 citation statements)

References 81 publications

(179 reference statements)

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“…By this hypothesis, a depletion of nucleoside pools signals DNA uptake, while an excess of nucleosides halts competence. In V. cholerae, recent evidence linking nucleoside uptake to competence bolsters such an argument and is described below (13).…”

Section: Nucleoside Sensing Systemmentioning

confidence: 99%

“…Liang et al demonstrated in El Tor strain C7258 that Δcrp mutants are reduced for transcription of the major autoinducer synthase gene cqsA (14). As both A1552 and C6706 ΔcqsA mutants are defective in DNA uptake (12,13), one effect of CRP on competence likely also includes modulation of QS (Fig. 2).…”

Section: Grammentioning

confidence: 99%

“…Second, natural competence is also fully abolished by deletion of the ASMscience.org/MicrobiolSpectrumcyaA gene encoding the adenylate cyclase enzyme, which catalyzes cAMP production. Finally, a Δcrp mutant is not competent for DNA uptake (6,12,13,76,77).…”

Section: Tfox: Mechanism Of Competence Genes Regulation and A Role Fomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Currently, genetic evidence supports the role of a chitin utilization system, a quorum-sensing system (QS), and a nucleoside scavenging system in the control of genes that encode components of the "competence apparatus" (6,12,13). Carbon catabolite repression (CCR), a global response to glucose starvation, impinges on all three systems (12,13,14). It is appreciated by studies of other naturally competent microbes that acquisition of extracellular DNA may serve in genome repair, nutrition, as well as for HGT [for reviews see (8,15,16)].…”

Section: Introductionmentioning

confidence: 99%

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Genetics of Natural Competence in Vibrio cholerae and other Vibrios

Antonova

Hammer

2015

Microbiol Spectr

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Many Gram-positive and Gram-negative bacteria can become naturally competent to take up extracellular DNA from the environment via a dedicated uptake apparatus. The genetic material that is acquired can (i) be used for nutrients, (ii) aid in genome repair, and (iii) promote horizontal gene transfer when incorporated onto the genome by homologous recombination, the process of “transformation.” Recent studies have identified multiple environmental cues sufficient to induce natural transformation in Vibrio cholerae and several other Vibrio species. In V. cholerae , nutrient limitation activates the cAMP receptor protein regulator, quorum-sensing signals promote synthesis of HapR-controlled QstR, chitin stimulates production of TfoX, and low extracellular nucleosides allow CytR to serve as an additional positive regulator. The network of signaling systems that trigger expression of each of these required regulators is well described, but the mechanisms by which each in turn controls competence apparatus genes is poorly understood. Recent work has defined a minimal set of genes that encode apparatus components and begun to characterize the architecture of the machinery by fluorescence microscopy. While studies with a small set of V. cholerae reference isolates have identified regulatory and competence genes required for DNA uptake, future studies may identify additional genes and regulatory connections, as well as revealing how common natural competence is among diverse V. cholerae isolates and other Vibrio species.

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Section: Nucleoside Sensing Systemmentioning

confidence: 99%

Section: Grammentioning

confidence: 99%

Section: Tfox: Mechanism Of Competence Genes Regulation and A Role Fomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetics of Natural Competence in Vibrio cholerae and other Vibrios

Antonova

Hammer

2015

Microbiol Spectr

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Vibrio cholerae: Measuring Natural Transformation Frequency

2014

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Many bacteria can become naturally competent to take up extracellular DNA across their outer and inner membranes by a dedicated competence apparatus. Whereas some studies show that the DNA delivered to the cytoplasm may be used for genome repair or for nutrition, it can also be recombined onto the chromosome by homologous recombination: a process called natural transformation. Along with conjugation and transduction, natural transformation represents a mechanism for horizontal transfer of genetic material, e.g., antibiotic resistance genes, which can confer new beneficial characteristics onto the recipient bacteria. Described here are protocols for quantifying the frequency of transformation for the human pathogen Vibrio cholerae, one of several Vibrio species recently shown to be capable of natural transformation. © 2014 by John Wiley & Sons, Inc.

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Small RNAs in the Vibrionaceae: an ocean still to be explored

Nguyen

Jacq

2014

WIREs RNA

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In bacteria, the discovery of noncoding small RNAs (sRNAs) as modulators of gene expression in response to environmental signals has brought new insights into bacterial gene regulation, including control of pathogenicity. The Vibrionaceae constitute a family of marine bacteria of which many are responsible for infections affecting not only humans, such as Vibrio cholerae but also fish and marine invertebrates, representing the major cause of mortality in farmed marine species. They are able to colonize many habitats, existing as planktonic forms, in biofilms or associated with various hosts. This high adaptability is linked to their capacity to generate genetic diversity, in part through lateral gene transfer, but also by varying gene expression control. In the recent years, several major studies have illustrated the importance of small regulatory sRNAs in the Vibrionaceae for the control of pathogenicity and adaptation to environment and nutrient sources such as chitin, especially in V. cholerae and Vibrio harveyi. The existence of a complex regulatory network controlled by quorum sensing has been demonstrated in which sRNAs play central roles. This review covers major advances made in the discovery and elucidation of functions of Vibrionaceae sRNAs within the last 10 years.

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Natural competence in Vibrio cholerae is controlled by a nucleoside scavenging response that requires CytR‐dependent anti‐activation

Cited by 51 publications

References 81 publications

Genetics of Natural Competence in Vibrio cholerae and other Vibrios

Genetics of Natural Competence in Vibrio cholerae and other Vibrios

Vibrio cholerae: Measuring Natural Transformation Frequency

Small RNAs in the Vibrionaceae: an ocean still to be explored

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