Natural Autotetraploid and Chromosomal Characteristics in the Subfamily Botiinae (Cypriniformes, Cobitinae) from Northeast Thailand

Kaewmad, Puntivar; Monthatong, Monthira; Supiwong, Weerayuth; Saowakoon, Samnao; Tanomtong, Alongklod

doi:10.1508/cytologia.79.299

Cited by 5 publications

(5 citation statements)

References 13 publications

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“…fasciatus and Y . lecontei deviated slightly from former descriptions [ 48 , 74 , 75 ] due to different morphological classification of some chromosomal pairs resulting likely from difficulties associated with small size of botiid chromosomes and their gradual transitions both in size and centromere position. Finally, the karyotypes of B .…”

Section: Discussioncontrasting

confidence: 61%

“…The current dataset along with the supposed age of botiid WGD makes it, on the other hand, difficult to draw any conclusions about the origin of this polyploidization (i.e., whether it was autopolyploidy or allopolyploidy). Despite some previous studies [ 48 ] presumed an autopolyploidization as the underlying mechanism using conventionally stained chromosomes only, it remained unclear on which facts they built this conclusion. Current data are, however, sufficient enough to reinforce the conclusion from our previous study [ 49 ] that S .…”

Section: Discussionmentioning

confidence: 95%

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Dynamics of tandemly repeated DNA sequences during evolution of diploid and tetraploid botiid loaches (Teleostei: Cobitoidea: Botiidae)

et al. 2018

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Polyploidization has played an important role in the evolution of vertebrates, particularly at the base of Teleostei–an enormously successful ray-finned fish group with additional genome doublings on lower taxonomic levels. The investigation of post-polyploid genome dynamics might provide important clues about the evolution and ecology of respective species and can help to decipher the role of polyploidy per se on speciation. Few studies have attempted to investigate the dynamics of repetitive DNA sequences in the post-polyploid genome using molecular cytogenetic tools in fishes, though recent efforts demonstrated their usefulness. The demonstrably monophyletic freshwater loach family Botiidae, branching to evolutionary diploid and tetraploid lineages separated >25 Mya, offers a suited model group for comparing the long-term repetitive DNA evolution. For this, we integrated phylogenetic analyses with cytogenetical survey involving Giemsa- and Chromomycin A3 (CMA3)/DAPI stainings and fluorescence in situ hybridization with 5S/45S rDNA, U2 snDNA and telomeric probes in representative sample of 12 botiid species.The karyotypes of all diploids were composed of 2n = 50 chromosomes, while majority of tetraploids had 2n = 4x = 100, with only subtle interspecific karyotype differences. The exceptional karyotype of Botia dario (2n = 4x = 96) suggested centric fusions behind the 2n reduction. Variable patterns of FISH signals revealed cases of intraspecific polymorphisms, rDNA amplification, variable degree of correspondence with CMA3+ sites and almost no phylogenetic signal. In tetraploids, either additivity or loci gain/loss was recorded. Despite absence of classical interstitial telomeric sites, large blocks of interspersed rDNA/telomeric regions were found in diploids only.We uncovered different molecular drives of studied repetitive DNA classes within botiid genomes as well as the advanced stage of the re-diploidization process in tetraploids. Our results may contribute to link genomic approach with molecular cytogenetic analyses in addressing the origin and mechanism of this polyploidization event.

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Section: Discussioncontrasting

confidence: 61%

Section: Discussionmentioning

confidence: 95%

Dynamics of tandemly repeated DNA sequences during evolution of diploid and tetraploid botiid loaches (Teleostei: Cobitoidea: Botiidae)

et al. 2018

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“…There is no evidence of sexual dimorphism of the chromosomes in this species which accord to the reports on C. apogon, C. enoplos, C. lagleri and C. repasson (Magtoon and Arai 1989, 1993, Donsakul et al 2005, Seetapan 2007. Similar to several cyprinid fishes, no cytologically distinguishable sex chromosome was found (Ueda et al 2006, Mani et al 2009, Monteiro et al 2009, Kaewmad et al 2014, Sukham et al 2014. It may be possible that sex chromosomes are at the initiation step of differentiation which cannot be detected by cytogenetic analyses (Na-Nakron 2000).…”

Section: Diploid Chromosome Number Fundamental Number and Karyotypementioning

confidence: 92%

A Report on Classical Cytogenetics of Hihgfin Barb Fish, <i>Cyclocheilichthys armatus</i> (Cypriniformes, Cyprinidae)

et al. 2018

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Conventional staining and Ag-NORs banding techniques were applied on the chromosomes of hihgfin barb fish, Cyclocheilichthys armatus. Specimens were collected from the Chao Phraya River, Thailand. The mitotic chromosome preparations were directly obtained from kidney tissues of eight males and eight females. The results revealed that the diploid chromosome numbers of C. armatus was 2n=50 and the fundamental number (NF) was 94 in both males and females. The karyotype consists of four large metacentric, six large submetacentric, two large acrocentric, eight medium metacentric, 12 medium submetacentric, 12 medium acrocentric and six medium telocentric chromosomes. No sex chromosome was observed. Ag-NORs indicating NORs were clearly exhibited at the region adjacent to telomere of the short arm of some chromosomes. The NORs located on chromosomes of the metacentric chromosome pair 3 and the submetacentric chromosome pair 7. The karyotype formula of C. armatus is 2n=50=L m 4 +L sm 6 +L a 2 +M m 8 +M sm 12 +M a 12 +M t 6. Cytogenetic data of cyprinid fishes can be used as the basic knowledge for enhancing the existing cytotaxonomic information and chromosome evolution of the Cyprinidae especially in the genus Cyclocheilichthys.

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“…It is possible that the fish's sex-chromosomes are dependent upon an initiation of differentiation. Therefore, chromosomes containing sex-determination genes cannot be found by cytogenetic analyses (Bertollo et al, 2004;Kaewmad et al, 2014;Kasiroek et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

First Report of Chromosome Analysis of Two Siganid Fishes (Perciformes, Siganidae)

Seetapan¹,

Pinmongkhonkul²

2020

CMUJNS

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This is the first report of chromosome analysis in the white-spotted rabbitfish(Siganus canalicutus) and the orange-spotted spinefoot (S. gattatus) from the Andaman Sea, Thailand. Kidney cell samples were taken from three male and three female fishes. The mitotic chromosome preparation was created through direct engineering on kidney cells. Conventional and Ag-NOR staining techniques were applied so as to stain the chromosomes. The results showed that the diploid chromosome number of both S. canalicutus and S. gattatus was 2n=48, that the fundamental number (NF) was 50 in S. canalicutus and 54 in S. gattatus. Among the chromosomes present, both acrocentric and telocentric ones were identified as 2-46 and 6-42 respectively. No heteromorphic chromosomes were observed which could be considered as sex-chromosomes. After Ag-NORs banding technique, a single pair of nucleolar organiser regions/ NORs was observed on the short arm telomeric region of large acrocentric chromosome Pair 1 in both species. The karyotype formulas could be deduced as: S. canalicutus 2n (48) = L a 2 +L t 22 +M t 16 +S t 8 S. gattatus 2n (48) = L a 4 +L t 18 +M a 2 +M t 22 +S t 2

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Natural Autotetraploid and Chromosomal Characteristics in the Subfamily Botiinae (Cypriniformes, Cobitinae) from Northeast Thailand

Cited by 5 publications

References 13 publications

Dynamics of tandemly repeated DNA sequences during evolution of diploid and tetraploid botiid loaches (Teleostei: Cobitoidea: Botiidae)

Dynamics of tandemly repeated DNA sequences during evolution of diploid and tetraploid botiid loaches (Teleostei: Cobitoidea: Botiidae)

A Report on Classical Cytogenetics of Hihgfin Barb Fish, <i>Cyclocheilichthys armatus</i> (Cypriniformes, Cyprinidae)

First Report of Chromosome Analysis of Two Siganid Fishes (Perciformes, Siganidae)

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