Natural and synthetic nanopores directing osteogenic differentiation of human stem cells

Greiner, Johannes F. W.; Gottschalk, Martin; Fokin, Nadine; Büker, Björn; Kaltschmidt, Bernhard Peter; Dreyer, Axel; Wähnert, Dirk; Kaltschmidt, Christian; Hütten, Andreas; Kaltschmidt, Barbara

doi:10.1016/j.nano.2019.01.018

Cited by 34 publications

(62 citation statements)

References 35 publications

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“…We previously reported the presence of a particularly interesting population of NCSCs within the respiratory epithelium of the inferior turbinate in the human nasal cavity [78]. Namely, ITSCs gave efficiently rise to glutamatergic [41] and dopaminergic neurons [82] but also to mesodermal derivates like chondrocytes, adipocytes, or osteocytes [80,81,85,86]. Namely, ITSCs gave efficiently rise to glutamatergic [41] and dopaminergic neurons [82] but also to mesodermal derivates like chondrocytes, adipocytes, or osteocytes [80,81,85,86].…”

Section: The Role Of Sex In Neuronal Development and Neural Stem Cellmentioning

confidence: 96%

“…Human NCSCs can be isolated from various adult tissues, such as skin [74,75], periodontium [76], or the nasal cavity [77][78][79]. Next to these characteristics, adult NCSCs display a broad differentiation capability into mesodermal and ectodermal cell types [74,77,[80][81][82][83]. Next to these characteristics, adult NCSCs display a broad differentiation capability into mesodermal and ectodermal cell types [74,77,[80][81][82][83].…”

Section: The Role Of Sex In Neuronal Development and Neural Stem Cellmentioning

confidence: 99%

“…Common characteristics of isolated NCSCs particularly include their easy accessibility, expression of Nestin, p75 NTR , and the NC markers SLUG, SNAIL, TWIST, SOX9, and SOX10 as well as the ability to self-renew and formation of neurospheres in vitro (reviewed in [66]. Next to these characteristics, adult NCSCs display a broad differentiation capability into mesodermal and ectodermal cell types [74,77,[80][81][82][83]. We previously reported the presence of a particularly interesting population of NCSCs within the respiratory epithelium of the inferior turbinate in the human nasal cavity [78].…”

Section: Adult Neural Crest-derived Stem Cells and Sex-specific Diffementioning

confidence: 99%

“…We demonstrated in several studies that such ITSCs are able to differentiate into a wide range of mesodermal and ectodermal cell types [78,81,84]. Namely, ITSCs gave efficiently rise to glutamatergic [41] and dopaminergic neurons [82] but also to mesodermal derivates like chondrocytes, adipocytes, or osteocytes [80,81,85,86]. Due to their extraordinary broad differentiation potential, NCSCs and particularly ITSCs seem an ideal model system for understanding potential sex-specific differences in endogenous differentiation and regeneration processes.…”

Section: Adult Neural Crest-derived Stem Cells and Sex-specific Diffementioning

confidence: 99%

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Sexual dimorphisms in adult human neural, mesoderm‐derived, and neural crest‐derived stem cells

et al. 2019

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Sexual dimorphisms contribute, at least in part, to the severity and occurrence of a broad range of neurodegenerative, cardiovascular, and bone disorders. In addition to hormonal factors, increasing evidence suggests that stem cell‐intrinsic mechanisms account for sex‐specific differences in human physiology and pathology. Here, we discuss sex‐related intrinsic mechanisms in adult stem cell populations, namely mesoderm‐derived stem cells, neural stem cells (NSCs), and neural crest‐derived stem cells (NCSCs), and their implications for stem cell differentiation and regeneration. We particularly focus on sex‐specific differences in stem cell‐mediated bone regeneration, in neuronal development, and in NSC‐mediated neuroprotection. Moreover, we review our own recently published observations regarding the sex‐dependent role of NF‐κB‐p65 in neuroprotection of human NCSC‐derived neurons and sex differences in NCSC‐related disorders, so‐called neurocristopathies. These observations are in accordance with the increasing evidence pointing toward sex‐specific differences in neurocristopathies and degenerative diseases like Parkinson's disease or osteoporosis. All findings discussed here indicate that sex‐specific variability in stem cell biology may become a crucial parameter for the design of future treatment strategies.

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Section: The Role Of Sex In Neuronal Development and Neural Stem Cellmentioning

confidence: 96%

Section: The Role Of Sex In Neuronal Development and Neural Stem Cellmentioning

confidence: 99%

Section: Adult Neural Crest-derived Stem Cells and Sex-specific Diffementioning

confidence: 99%

Section: Adult Neural Crest-derived Stem Cells and Sex-specific Diffementioning

confidence: 99%

See 2 more Smart Citations

Sexual dimorphisms in adult human neural, mesoderm‐derived, and neural crest‐derived stem cells

et al. 2019

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“…Besides, the enhanced cell adhesion on the nanoporous surface, authors proved the nanotopography capability to induce the osteogenic differentiation of MSCs (Figure ). Another recent study conducted by Greiner et al () demonstrated for the first time that 31.93 ± 0.97 nm pores present within endogenous collagen Type I fibers are sufficient to induce the osteogenic differentiation of human stem cells. A collagen‐like scaffold which mimics the collagen pore structures was developed by self‐assembly of silicon dioxide (SiO 2 ) nanoparticles linked together by a thermally induced crosslinking reaction of oleic acid molecules (Dreyer et al, ).…”

Section: Nanoporesmentioning

confidence: 96%

Reviewing recently developed technologies to direct cell activity through the control of pore size: From the macro‐ to the nanoscale

2019

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Scaffold pore size plays a fundamental role in the regeneration of new tissue since it has been shown to direct cell activity in situ. It is well known that cellular response changes in relation with pores diameter. Consequently, researchers developed efficient approaches to realize scaffolds with controllable macro‐, micro‐, and nanoporous architecture. In this context, new strategies aiming at the manufacturing of scaffolds with multiscale pore networks have emerged, in the attempt to mimic the complex hierarchical structures found in living systems. In this review, we aim at providing an overview of the fabrication methods currently adopted to realize scaffolds with controlled, multisized pores highlighting their specific influence on cellular activity.

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Cost and Time Effective Lithography of Reusable Millimeter Size Bone Tissue Replicas With Sub‐15 nm Feature Size on A Biocompatible Polymer

Liu

Zanut

Sladkova‐Faure

et al. 2021

Adv Funct Materials

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The ability to replicate the microenvironment of biological tissues creates unique biomedical possibilities for stem cell applications. Current fabrication methods are limited by either the control on feature size and shape, or by the throughput and size of the replicas. Here, a novel platform is reported that combines thermal scanning probe lithography (tSPL) with innovative methodologies for the low‐cost and high‐throughput nanofabrication of large area quasi‐3D bone tissue replicas with high fidelity, sub‐15 nm lateral precision, and sub‐2 nm vertical resolution. This bio‐tSPL platform features a biocompatible polymer resist that withstands multiple cell culture cycles, allowing the reuse of the replicas, further decreasing costs and fabrication times. The as‐fabricated replicas support the culture and proliferation of human induced mesenchymal stem cells, which display broad therapeutic and biomedical potential. Furthermore, it is demonstrated that bio‐tSPL can be used to nanopattern the bone tissue replicas with amine groups, for subsequent tissue‐mimetic biofunctionalization. The achieved level of time and cost‐effectiveness, as well as the cell compatibility of the replicas, make bio‐tSPL a promising platform for the production of tissue‐mimetic replicas to study stem cell‐tissue microenvironment interactions, test drugs, and ultimately harness the regenerative capacity of stem cells and tissues for biomedical applications.

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Natural and synthetic nanopores directing osteogenic differentiation of human stem cells

Cited by 34 publications

References 35 publications

Sexual dimorphisms in adult human neural, mesoderm‐derived, and neural crest‐derived stem cells

Sexual dimorphisms in adult human neural, mesoderm‐derived, and neural crest‐derived stem cells

Reviewing recently developed technologies to direct cell activity through the control of pore size: From the macro‐ to the nanoscale

Cost and Time Effective Lithography of Reusable Millimeter Size Bone Tissue Replicas With Sub‐15 nm Feature Size on A Biocompatible Polymer

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