Natural adaption to pigs of a Taiwanese isolate of foot‐and‐mouth disease virus

Dunn, Chris; Donaldson, Alex

doi:10.1136/vr.141.7.174

Cited by 131 publications

(80 citation statements)

References 3 publications

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Section: Cell Line and Virusesmentioning

confidence: 99%

“…The swine-adapted strain of FMD virus which occurred in Taiwan in 1997 killed more than 4 million pigs, hit related industries heavily and made Taiwan losing FMD free status [27]. To regain the free status, an eradication campaign has been implemented since then.The FMD virus, O/Taiwan/97 (O/97), produced high morbidity and mortality in swine but did not affect cattle [5]. The distinct host tropism was demonstrated that the mutated 3A coding region of O/97 played a major role in the attenuation of this virus in bovine [3].…”

mentioning

confidence: 99%

“…The FMD virus, O/Taiwan/97 (O/97), produced high morbidity and mortality in swine but did not affect cattle [5]. The distinct host tropism was demonstrated that the mutated 3A coding region of O/97 played a major role in the attenuation of this virus in bovine [3].…”

mentioning

confidence: 99%

“…Two virus isolates of serotype O, the O/97, O/Taiwan/KM1/99 (O/99), and SVDV were used in this study. The O/97 virus used as the immunogen, target of IFA and neutralization assay is a porcinophilic virus strain, which does not infect ruminants in natural route [5].The O/99 virus, isolated from the Chinese yellow cattle in Kinmen Island during the 1999 outbreaks [11], was used as the target in IFA to study the specificity of MAbs. A SVDV Taiwanese field isolate was a target in IFA for characterizing the MAbs.…”

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Study on the Porcinophilic Foot-and-Mouth Disease Virus I. Production and Characterization of Monoclonal Antibodies against VP1

Cheng¹,

Liang

et al. 2006

J. Vet. Med. Sci.

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ABSTRACT. Monoclonal antibodies (MAbs) reported here were produced against the porcinophilic foot-and-mouth disease virus (FMDV) that caused the devastating swine disease on 1997 in Taiwan. A panel (25) of MAbs were found to react with VP1 of O/Taiwan/97 (O/ 97) by ELISA with various potencies. The biological identities of these VP1 reacting MAbs, such as neutralization activity, isotype and capability to distinguish between two serotype O FMDVs, O/97 and O/Taiwan/KM1/99 (O/99), were further analyzed. Eleven out of the total eighteen O/97 neutralizing MAbs were able to neutralize heterologous O/99. Eight O/97 neutralizing and five non-neutralizing MAbs could differentiate two serotype O FMDVs by immunofluorescence assay (IFA) implied that these thirteen MAbs recognized O/ 97 specific epitope(s). Furthermore, reactivities of the VP1 reacting MAbs with a 29 amino acids synthetic peptide (P29) representing the βG-βH loop of VP1 were analyzed by ELISA and fourteen were found positive. MAb clone Q10E-3 reacting strongest with VP1 and P29, neutralizing both but not differentiating two serotype O viruses suggested that the antibody binding site might involve the RGD motif and its C terminal conserved region on βG-βH loop. MAbs with diverse characters presented in this study were the first raised against porcinophilic FMDV. The complete set of MAbs may be used for further studies of vaccine, diagnostic methods, prophylaxis, etiological and immunological researches on FMDV. KEY WORDS: ELISA, foot-and-mouth disease virus, monoclonal antibody, neutralizing activity, VP1.J. Vet. Med. Sci. 68(8): 859-864, 2006 All cloven-hoofed species are susceptible to foot-andmouth disease (FMD) that is considered the most contagious animal disease. The outbreaks of FMD in Argentina, Europe, Asia, South Africa and Uruguay [8] have brought to world attention the devastating effects of the disease in a naïve population and economic costs of control and eradication. The swine-adapted strain of FMD virus which occurred in Taiwan in 1997 killed more than 4 million pigs, hit related industries heavily and made Taiwan losing FMD free status [27]. To regain the free status, an eradication campaign has been implemented since then.The FMD virus, O/Taiwan/97 (O/97), produced high morbidity and mortality in swine but did not affect cattle [5]. The distinct host tropism was demonstrated that the mutated 3A coding region of O/97 played a major role in the attenuation of this virus in bovine [3]. Besides, limited biologic character of this porcinophilic virus was known. With the high specificity of antigen (Ag) recognition, monoclonal antibody (MAb) has been applied on the field of determination of antigenic epitope, diagnostic development, vaccine research and studies of viral pathogenesis for years. Therefore, production of MAbs against the porcinophilic FMDV would be beneficial to both basic and applied researches.This paper described the production of a panel of MAbs against porcinophilic O/97 strain FMDV. Their reactivities with VP1 were determi...

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Section: Cell Line and Virusesmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Study on the Porcinophilic Foot-and-Mouth Disease Virus I. Production and Characterization of Monoclonal Antibodies against VP1

Cheng¹,

Liang

et al. 2006

J. Vet. Med. Sci.

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“…The study of the VP1 sequences in the international DNA data bank (NCBI) indicated that the O/Taiwan/ 97 virus was closely related to the viruses seen in Russia (O/ 1685/Moscow/Russia/95), Hong Kong and the Philippines [9; our unpublished data]. The O/Taiwan/97 virus is a pigadapted strain of FMD virus which does not infect cattle by natural route [6] .…”

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Chaaracteristics of Foot and Mouth Disease Virus in Taiwan.

Huang

Jong

Lin

2000

The Journal of Veterinary Medical Science

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ABSTRACT. Since March 1997 two strains of foot and mouth disease (FMD) virus have found their way into Taiwan, causing severe outbreaks in pigs and in Chinese yellow cattle. Outbreaks occurred in March 1997 were caused by a pig-adapted virus strain (O/Taiwan/ 97) which did not infect other species of cloven-hoofed animals by natural route. The epidemic spread over the whole region of Taiwan within two months and the aftermath was 6,147 pig farms infected and 3,850,746 pigs destroyed. In June 1999, the second strain of FMD virus (O/Taiwan/99) was isolated from the Chinese yellow cattle in the Kinmen Prefecture and in the western part of Taiwan. By the end of 1999, Chinese yellow cattle were the only species infected and those infected cattle did not develop pathological lesions. Seroconversions of serum neutralization antibody and on non-structural protein (NSP) antibodies were the best indicators for infection in non-vaccinated herds. The infected animals, however, excreted infectious levels of virus to infect new hosts. Based on the detection of the specific antibody to FMD virus, and virus isolation from oesophageal-pharyngeal (OP) fluid samples, ten herds of Chinese yellow cattle located in Kinmen and Taiwan were declared to have been infected. During the period of January to March 2000, however, five outbreaks caused by FMD virus similar to the O/Taiwan/99 virus occurred in four prefectures of Taiwan. The infected species included goats, Chinese yellow cattle and dairy cattle. Those outbreaks have caused high mortality in goat kids under two weeks old and also developed typical clinical signs of infection in dairy cattle.KEY WORDS: Chinese yellow cattle, foot and mouth disease virus, non-structural protein, serotype O, VP1 gene.

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