Natronolimnobius sulfurireducens sp. nov. and Halalkaliarchaeum desulfuricum gen. nov., sp. nov., the first sulfur-respiring alkaliphilic haloarchaea from hypersaline alkaline lakes

Sorokin, Dimitry Y.; Yakimov, Mikhail M.; Messina, Enzo; Merkel, Alexander Y.; Bale, Nicole J.; Damsté, Jaap S. Sinninghe

doi:10.1099/ijsem.0.003506

Cited by 31 publications

(14 citation statements)

References 37 publications

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“…aegyptiacus [4] and Nlb. sulfurireducens [5,6] with even lower distances of their 16S rRNA gene from the previously described species from Inner Mongolia (94.5-96 % identity). However, with the use of a phylogenomic approach as an alternative for the single conservative molecular marker-based paradigm it is becoming excessively clear that in many fast-evolving lineages, such as the Natrialbales in the haloarchaea, the 16S rRNA gene-based phylogeny does not reliably resolve the evolutionary history of their members.…”

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confidence: 73%

“…The tests for polysaccharide utilization and anaerobic growth with sulfur/DMSO were run as described previously [6,19]. Additional polar lipid and respiratory quinone analyses were also performed for Nlb.…”

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confidence: 99%

“…The most important phenotypic property of Nlb. sulfurireducens, found in hypersaline soda lakes world-wide [5,6], is its ability to grow anaerobically by sulfur respiration using diverse electron donors, including hydrogen, formate, C 4 -C 8 fatty acids, pyruvate and peptone/yeast extract. It also accumulates large amounts of poly-β-hydroxyalkanoates during anaerobic growth, a property not reported for any other member of the class Halobacteria.…”

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confidence: 99%

“…Comparative properties of the species of Natronolimnobius, Natronolimnohabitans and Natrarchaeobaculum based on literature data[1,2,[4][5][6]19] and cross-examination of the type strains…”

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confidence: 99%

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Reclassification of the genus Natronolimnobius: proposal of two new genera, Natronolimnohabitans gen. nov. to accommodate Natronolimnobius innermongolicus and Natrarchaeobaculum gen. nov. to accommodate Natronolimnobius aegyptiacus and Natronolimnobius sulfurireducens

Sorokin

Merkel

Messina

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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The genus Natronolimnobius , currently including four species, is a member of the order Natrialbales , class Halobacteria, and consists of obligately alkaliphilic and extremely halophilic members found exclusively in highly alkaline hypersaline soda lakes. The species were classified into this genus mostly based on phylogenetic analysis of the 16S rRNA gene. However, a more advanced phylogenomic reconstruction based on 122 conserved single-copy archaeal protein markers clearly indicates a polyphyletic origin of the species included into this genus, thus warranting its reclassification into three separate genera. We therefore propose to transfer Nlb. innermongolicus (type strain N-1311) to a new genus Natronolimnohabitans as Nlh. innermongolicus comb. nov. and to transfer Nlb. aegyptiacus (type strain JW/NM-HA 15) and Nlb. sulfurireducens (type strain AArc1) to a new genus Natrarchaeobaculum as Nbl. aegyptiacum comb. nov. and Nbl. sulfurireducens comb. nov. The phylogenomic differentiation of these four species is also supported by the ANI/AAI distances and unique phenotypes. The most important physiological differences includes a previously unreported ability for cellulose and xylan utilization in Nlb. baerhuensis, thermophily in Nbl. aegyptiacus and anaerobic sulfur respiration in Nbl. sulfurireducens. We further present an emended description of Natronolimnobius baerhuensis .

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confidence: 73%

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confidence: 99%

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confidence: 99%

“…Comparative properties of the species of Natronolimnobius, Natronolimnohabitans and Natrarchaeobaculum based on literature data[1,2,[4][5][6]19] and cross-examination of the type strains…”

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Reclassification of the genus Natronolimnobius: proposal of two new genera, Natronolimnohabitans gen. nov. to accommodate Natronolimnobius innermongolicus and Natrarchaeobaculum gen. nov. to accommodate Natronolimnobius aegyptiacus and Natronolimnobius sulfurireducens

Sorokin

Merkel

Messina

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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“…aegyptiacus (Zhao et al, 2018) and Nl. sulfurireducens (Sorokin et al, 2019b) misidentified, but also appear to represent two novel genera within the family Natrialbaceae.…”

Section: Five Potentially Novel Genera Within Subclade 5dmentioning

confidence: 99%

Phylogenetic relationships among haloalkaliphilic archaea of the familyNatrialbaceae

Siddaramappa

2020

Preprint

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Likewise, Halostagnicola larsenii, Halostagnicola kamekurae, and Halostagnicola sp. A56 were co-located on a separate branch in the phylogenetic tree (Fig. 1). The ANI/AAI values between Hs. larsenii, the type species of the genus, and Hs. kamekurae and Halostagnicola sp. A56 were 87.16%/86.61% and 86.88%/86.84%, respectively. Therefore, the proposals that Halostagnicola is a novel genus (Castillo et al., 2006b), and that Hs. kamekurae is a novel species of this genus (Nagaoka et al., 2010), are valid. It is also likely that Halostagnicola sp. A56, whose ANI/AAI value with Hs. kamekurae was 92.10%/91.64%, represents a novel species that is yet to be characterized and named. Furthermore, Natronococcus occultus, Natronococcus amylolyticus, and Natronococcus jeotgali were co-located on a separate branch in the phylogenetic tree (Fig. 1). The ANI/AAI values between Nc. occultus, the type species of the genus, and Nc. amylolyticus and Nc. jeotgali were 85.82%/84.69% and 87.01%/84.22%, respectively. Hence, the proposals that Natronococcus is a novel genus (Tindall et al., 1984), and that Nc. amylolyticus and Nc. jeotgali are novel species of this genus (Kanal et al., 1995;Roh et al., 2007), are valid. The genus Natrialba and a potentially novel genus within subclade 5AThe novel genus Natrialba containing a single species was proposed by Kamekura and Dyall-Smith (1995). Since then, the names of five species of Natrialba have been validly published. As of July 2019, the genome sequences of all six species of Natrialba have been sequenced, with Natrialba magadii ATCC 43099 T being sequenced twice (Table 1). Subclade 5A in the phylogenetic tree ( Fig. 1) contained two distinct branches, each having three species of Natrialba. The ANI/AAI value between Natrialba taiwanensis and Natrialba aegyptia was 94.83%/95.00%. Since the DNA-DNA hybridization level between Na. taiwanensis and Na.aegyptia was reported to be 66% (Hezayen et al., 2001), it is likely that these two species are very closely related. The DNA-DNA hybridization level between Natrialba asiatica, the type species of the genus, and Na. taiwanensis was reported to be 55.8% (Hezayen et al., 2001), indicating that they are two different species. This is supported by the fact that the ANI/AAI value between these archaea was 93.12%/92.95%. Furthermore, the ANI/AAI value between Natrialba chahannaoensis and Na. magadii was 88.84%/88.17%, the ANI/AAI value between Na. magadii and Natrialba hulunbeirensis was 89.40%/89.61%, and the ANI/AAI value between Na. chahannaoensis and Na. hulunbeirensis was 88.78%/89.90%. This result confirms the proposal by Xu et al. (2001) that Na. chahannaoensis and Na. hulunbeirensis are distinct from each other, and from Na. magadii.However, the ANI/AAI values between Na. asiatica and Na. chahannaoensis, Na. magadii, Na. hulunbeirensis, and Natrialba sp. SSL1 were 82.17%/76.00%, 82.58%/76.21%, 82.55%/76.30%, and 82.75%/75.75%. Interestingly, Hezayen et al. (2001) had reported that the 16S rDNA sequence similarity between Na. asiatica and N...

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Halalkaliarchaeum

Sorokin¹,

Merkel²,

Yakimov³

et al. 2020

Bergey's Manual of Systematics of Archaea and Bacteria

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Hal.al.ka.li.ar.chae'um. Gr. masc. n. hals , halos salt; N.L. n. alkali (from Arabic article al and the and Arabic n. qaliy ashes of saltwort); N.L. neut. n. archaeum (from Gr. masc. adj. archaios ancient) archaeon; N.L. neut. n. Halalkaliarchaeum a haloalkaliphilic archaeon. Euryarchaeota / Halobacteria / Haloferacales / Halorubraceae / Halalkaliarchaeum The genus Halalkaliarchaeum , classified within the family Halorubraceae , order Haloferacales , and within the class Halobacteria , consists of pleomorphic flattened rod‐shaped nonmotile cells with a thin monolayer cell wall. Colonies on agar plates incubated aerobically are red‐orange. The single described species of the genus is extremely halophilic, requiring 2.5–5 M Na + and growing optimally at 4 M Na + , and alkaliphilic, growing at pH 7.8–9.5 with an optimum at pH 8.8–9.0. The optimum temperature is 43°C. It is facultative anaerobe, growing aerobically as a chemoorganoheterotroph on pyruvate and peptone–yeast extract or anaerobically as a chemolithoheterotroph, using H 2 or formate as electron donors, elemental sulfur as electron acceptor, and yeast extract as carbon source. Sugars are not used as carbon and energy source. The respiratory quinone is MK‐8(H 2 ). The major polar lipids are C 20 –C 20 and C 25 –C 20 diether dialkylglycerol ethers, with phosphatidylglycerol phosphate methyl ester (PGP‐Me) and phosphatidylglycerol (PG) as the dominant polar lipids. DNA G + C content (mol%) : 63.1 (genome sequence). Type species : Halalkaliarchaeum desulfuricum Sorokin et al. 2019 VP .

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Natronolimnobius sulfurireducens sp. nov. and Halalkaliarchaeum desulfuricum gen. nov., sp. nov., the first sulfur-respiring alkaliphilic haloarchaea from hypersaline alkaline lakes

Cited by 31 publications

References 37 publications

Reclassification of the genus Natronolimnobius: proposal of two new genera, Natronolimnohabitans gen. nov. to accommodate Natronolimnobius innermongolicus and Natrarchaeobaculum gen. nov. to accommodate Natronolimnobius aegyptiacus and Natronolimnobius sulfurireducens

Reclassification of the genus Natronolimnobius: proposal of two new genera, Natronolimnohabitans gen. nov. to accommodate Natronolimnobius innermongolicus and Natrarchaeobaculum gen. nov. to accommodate Natronolimnobius aegyptiacus and Natronolimnobius sulfurireducens

Phylogenetic relationships among haloalkaliphilic archaea of the familyNatrialbaceae

Halalkaliarchaeum

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