Natronobacillus azotifigens gen. nov., sp. nov., an anaerobic diazotrophic haloalkaliphile from soda-rich habitats

Sorokin, Ivan D.; Zadorina, E. V.; Кравченко, И. К.; Boulygina, Eugenia S.; Tourova, T. P.; Sorokin, Dimitry Y.

doi:10.1007/s00792-008-0188-0

Cited by 40 publications

(9 citation statements)

References 31 publications

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“…The phylogenetic relationships among the three WHC2b shotgun sequencing reads and two Lost City shotgun sequencing reads with potential ACS homologs are displayed in Figure A2 in Appendix. All three WHC2b sequences cluster with the ACS of Dethiobacter alkaliphilus , a H 2 -oxidizing autotrophic Clostridia that can use acetate as a carbon source but not as an energy source (Sorokin et al, 2008). To our knowledge, there is no published evidence that D. alkaliphilus can carry out acetogenesis.…”

Section: Resultsmentioning

confidence: 99%

Metagenomic Evidence for H2 Oxidation and H2 Production by Serpentinite-Hosted Subsurface Microbial Communities

Brazelton¹,

Nelson²,

Schrenk³

2012

Front. Microbio.

180

218

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Ultramafic rocks in the Earth’s mantle represent a tremendous reservoir of carbon and reducing power. Upon tectonic uplift and exposure to fluid flow, serpentinization of these materials generates copious energy, sustains abiogenic synthesis of organic molecules, and releases hydrogen gas (H2). In order to assess the potential for microbial H2 utilization fueled by serpentinization, we conducted metagenomic surveys of a marine serpentinite-hosted hydrothermal chimney (at the Lost City hydrothermal field) and two continental serpentinite-hosted alkaline seeps (at the Tablelands Ophiolite, Newfoundland). Novel [NiFe]-hydrogenase sequences were identified at both the marine and continental sites, and in both cases, phylogenetic analyses indicated aerobic, potentially autotrophic Betaproteobacteria belonging to order Burkholderiales as the most likely H2-oxidizers. Both sites also yielded metagenomic evidence for microbial H2 production catalyzed by [FeFe]-hydrogenases in anaerobic Gram-positive bacteria belonging to order Clostridiales. In addition, we present metagenomic evidence at both sites for aerobic carbon monoxide utilization and anaerobic carbon fixation via the Wood–Ljungdahl pathway. In general, our results point to H2-oxidizing Betaproteobacteria thriving in shallow, oxic–anoxic transition zones and the anaerobic Clostridia thriving in anoxic, deep subsurface habitats. These data demonstrate the feasibility of metagenomic investigations into novel subsurface habitats via surface-exposed seeps and indicate the potential for H2-powered primary production in serpentinite-hosted subsurface habitats.

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Section: Resultsmentioning

confidence: 99%

Metagenomic Evidence for H2 Oxidation and H2 Production by Serpentinite-Hosted Subsurface Microbial Communities

Brazelton¹,

Nelson²,

Schrenk³

2012

Front. Microbio.

180

218

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“…3b2). These organisms are represented by two groups of the Firmicutes: a moderate salt-tolerant Anaerobacillus diazotrophicus ( reclassified from Bacillus alkalidiazotrophicus) (Sorokin et al 2008d) and a highly salt-tolerant Natronobacillus azotifigans (Sorokin et al 2008e). Furthermore, the microbial activity and presence of the nifH gene, encoding a nitrogenase, have also been detected in two other soda lake anaerobes: the iron-reducing Geoalkalibacter ferrihydriticus (Zavarzina et al 2006) and the cellulolytic Clostridium alkalicellulosi (Zhilina et al 2005a).…”

Section: Cultured Diversity and Their Role In Biogeochemical Cyclesmentioning

confidence: 99%

“…alkalilacustre Lake Khadyn (Tuva Region, Siberia, Russia)Zavarzina et al (2009)NA Cyanospira C. rippkae Lake Magadi (Kenya)Florenzano et al (1985)NA C. capsulata Lake Magadi (Kenya)Florenzano et al (1985)NA Clostridium Cl. alkalicellum Lake Verkhnee Beloe (Buryatiya, Russia)Zhilina et al (2005a, b) PD5307447332 Natronobacillus N. azotofigens Kulunda Steppe (Siberia, Russia)Sorokin et al (2008e)NA Tindallia Td. magadii Lake Magadi (Kenya)Kevbrin et al (1998) NA Nitrobacter Nb.…”

Section: Introductionmentioning

confidence: 99%

Microbial diversity and biogeochemical cycling in soda lakes

et al. 2014

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Soda lakes contain high concentrations of sodium carbonates resulting in a stable elevated pH, which provide a unique habitat to a rich diversity of haloalkaliphilic bacteria and archaea. Both cultivation-dependent and -independent methods have aided the identification of key processes and genes in the microbially mediated carbon, nitrogen, and sulfur biogeochemical cycles in soda lakes. In order to survive in this extreme environment, haloalkaliphiles have developed various bioenergetic and structural adaptations to maintain pH homeostasis and intracellular osmotic pressure. The cultivation of a handful of strains has led to the isolation of a number of extremozymes, which allow the cell to perform enzymatic reactions at these extreme conditions. These enzymes potentially contribute to biotechnological applications. In addition, microbial species active in the sulfur cycle can be used for sulfur remediation purposes. Future research should combine both innovative culture methods and state-of-the-art ‘meta-omic’ techniques to gain a comprehensive understanding of the microbes that flourish in these extreme environments and the processes they mediate. Coupling the biogeochemical C, N, and S cycles and identifying where each process takes place on a spatial and temporal scale could unravel the interspecies relationships and thereby reveal more about the ecosystem dynamics of these enigmatic extreme environments.

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“…The maximal growth temperature with galacturonic acid as substrate was 41°C. Catalase activity was present, but at a much lower level than in the aerotolerant fermentative alkaliphile Natronobacillus (Sorokin et al 2008). …”

Section: Resultsmentioning

confidence: 99%

Natronoflexus pectinivorans gen. nov. sp. nov., an obligately anaerobic and alkaliphilic fermentative member of Bacteroidetes from soda lakes

et al. 2011

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Anaerobic enrichment with pectin at pH 10 and moderate salinity inoculated with sediments from soda lakes of the Kulunda Steppe (Altai, Russia) resulted in the isolation of a novel member of the Bacteroidetes, strain AP1T. The cells are long, flexible, Gram-negative rods forming pink carotenoids. The isolate is an obligate anaerobe, fermenting various carbohydrates to acetate and succinate. It can hydrolyze and utilize pectin, xylan, starch, laminarin and pullulan as growth substrates. Growth is possible in a pH range from 8 to 10.5, with an optimum at pH 9.5, and at a salinity range from 0.1 to 2 M Na+. Phylogenetic analysis based on 16S rRNA sequences placed the isolate into the phylum Bacteroidetes as a separate lineage within the family Marinilabilaceae. On the basis of distinct phenotype and phylogeny, the soda lake isolate AP1T is proposed to be assigned in a new genus and species Natronoflexus pectinivorans (=DSM24179T = UNIQEM U807T).Electronic supplementary materialThe online version of this article (doi:10.1007/s00792-011-0399-7) contains supplementary material, which is available to authorized users.

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Natronobacillus azotifigens gen. nov., sp. nov., an anaerobic diazotrophic haloalkaliphile from soda-rich habitats

Cited by 40 publications

References 31 publications

Metagenomic Evidence for H2 Oxidation and H2 Production by Serpentinite-Hosted Subsurface Microbial Communities

Metagenomic Evidence for H2 Oxidation and H2 Production by Serpentinite-Hosted Subsurface Microbial Communities

Microbial diversity and biogeochemical cycling in soda lakes

Natronoflexus pectinivorans gen. nov. sp. nov., an obligately anaerobic and alkaliphilic fermentative member of Bacteroidetes from soda lakes

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