Natriuretic and diuretic effects of salmon calcitonin in rats

Keeler, R.; Walker, Valerie; Copp, D. Harold

doi:10.1139/y70-122

Cited by 24 publications

(12 citation statements)

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“…62 A high concentration of CT affected calcium and magnesium excretion and even had a diuretic effect. 18,63,64 However, the concentration of CT used in our study was equivalent to the plasma concentration of endogenous thyrocalcitonin that does not cause phosphaturia and natriuresis. 16,18 At this lower concentration, we show that AQP2 membrane accumulation in the kidney is stimulated, indicating that CT affects principal cells and not only intercalated cells, as suggested earlier.…”

Section: Discussionmentioning

confidence: 91%

Calcitonin Has a Vasopressin-like Effect on Aquaporin-2 Trafficking and Urinary Concentration

Bouley¹,

Lu²,

Nunes³

et al. 2011

Journal of the American Society of Nephrology

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The most common cause of hereditary nephrogenic diabetes insipidus is a nonfunctional vasopressin (VP) receptor type 2 (V2R). Calcitonin, another ligand of G-protein-coupled receptors, has a VP-like effect on electrolytes and water reabsorption, suggesting that it may affect AQP2 trafficking. Here, calcitonin increased intracellular cAMP and stimulated the membrane accumulation of AQP2 in LLC-PK1 cells. Pharmacologic inhibition of protein kinase A (PKA) and deficiency of a critical PKA phosphorylation site on AQP2 both prevented calcitonin-induced membrane accumulation of AQP2. Fluorescence assays showed that calcitonin led to a 70% increase in exocytosis and a 20% decrease in endocytosis of AQP2.Immunostaining of rat kidney slices demonstrated that calcitonin induced a significant redistribution of AQP2 to the apical membrane of principal cells in cortical collecting ducts and connecting segments but not in the inner stripe or inner medulla. Calcitonin-treated VP-deficient Brattleboro rats had a reduced urine flow and two-fold higher urine osmolality during the first 12 hours of treatment compared with control groups. Although this VP-like effect of calcitonin diminished over the following 72 hours, the tachyphylaxis was reversible. Taken together, these data show that calcitonin induces cAMP-dependent AQP2 trafficking in cortical collecting and connecting tubules in parallel with an increase in urine concentration. This suggests that calcitonin has a potential therapeutic use in nephrogenic diabetes insipidus.

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Section: Discussionmentioning

confidence: 91%

Calcitonin Has a Vasopressin-like Effect on Aquaporin-2 Trafficking and Urinary Concentration

Bouley¹,

Lu²,

Nunes³

et al. 2011

Journal of the American Society of Nephrology

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“…Acute clearance studies, each lasting approximately 6-8 h, were performed on female mongrel dogs weighing [16][17][18][19][20][21][22] kg which had been thyroparathyroidectomized (TPTX) at least 3 days before their use in an experimental study. Completeness of glandular ablation was confirmed by a postoperative decline in serum calcium concentration of at least 30%o, and thyroid hormone was replaced as synthroid, 0.3 mg/day by mouth.…”

Section: Methodsmentioning

confidence: 99%

“…An endotracheal tube was passed anfd the dogs were ventilated with a Harvard respirator. Inulin, p-aminohippurate (PAH) and aqueous vasopressin (ADH) were infused in physiological saline solution at a rate of 1 ml/min in quantities sufficient to provide plasma levels of [20][21][22][23][24][25][26][27][28][29][30] mg/100 ml and 1-3 mg/100 ml for the former two substances, and to deliver 30-60 mU/kg per h of the latter. After an equilibration period of 50-60 min, the following experimental maneuvers were performed.…”

Section: Methodsmentioning

confidence: 99%

Study of the Renal Tubular Interactions of Thyrocalcitonin, Cyclic Adenosine 3′, 5′ -Monophosphate, 25-Hydroxycholecalciferol, and Calcium Ion

Puschett¹,

Beck²,

Jelonek³

et al. 1974

J. Clin. Invest.

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A B S T R A C T Acute clearance studies were performed in thyroparathyroidectomized animals to determine the actions and interactions of thyrocalcitonin (TCT), cyclic adenosine 3'5'-monophosphate (cAMP), 25-hydroxycholecalciferol (25HCC), and calcium ion on the reabsorption of phosphate, calcium, sodium, and potassium by the kidney. The infusion of 25HCC in a dosage of 60 U/h to moderately saline-expanded animals (2.5% body weight) induced a fall in the excretion of all of the ions under study after 90-120 min similar to that observed in previous experiments from this laboratory. Mean decrements in fractional excretion were: phosphate, 42.0% (P < 0.005); calcium, 25.0% (P < 0.005); sodium, 23.4% (P < 0.001); and potassium, 14.7% (P < 0.005). The superimposition of either porcine or salmon TCT (1-100 MRC U/h for 2 h) resulted in no further alterations in electrolyte excretion. However, the infusion of TCT during steady-state saline expansion, before the administration of 25HCC, obviated the renal transport effects of the vitamin D metabolite. Both in the latter studies, as well as those in whiicl similar doses of TCT were given to hydropenic animnals, the hormone itself failed to induce any consistent alteration in electrolyte excretion. Cyclic AMP (50 mg/h) caused an increase in the excretion of phosphate, sodium, and potassium and no change in calcium excretion. Like TCT, the nucleotide blocked the action of 25HCC on the kidney. Raising the mean level of serum ultrafilterable calcium to 3.02±0.25 mEq/liter from 1.62±0

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“…In the rat extracted salmon calcitonin produced a marked increase in sodium excretion while increasing phosphate excretion only slightly (Keeler, Walker & Copp, 1970). Urinary magnesium fell, whereas calcium excretion decreased with small doses but was increased by a large dose.…”

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confidence: 95%

The Effects of Synthetic Human and Salmon Calcitonins on Electrolyte Excretion in the Rat

Williams¹,

Matthews²,

Moseley³

et al. 1972

Clinical Science

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S U M M A R Y1. The effects on renal electrolyte excretion of human and salmon synthetic calcitonins were studied in the rat.2. As little as 50 ng of salmon calcitonin produced an increase in sodium excretion, but human calcitonin in doses up to 15 pg had only a slight effect.3. Small doses of both hormones decreased urinary calcium and magnesium. Larger doses of salmon calcitonin produced less depression of calcium and magnesium excretion even in the presence of a greater fall in plasma calcium.4. The mechanisms responsible for these effects and the possible therapeutic implications are discussed.

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Natriuretic and diuretic effects of salmon calcitonin in rats

Cited by 24 publications

References 0 publications

Calcitonin Has a Vasopressin-like Effect on Aquaporin-2 Trafficking and Urinary Concentration

Calcitonin Has a Vasopressin-like Effect on Aquaporin-2 Trafficking and Urinary Concentration

Study of the Renal Tubular Interactions of Thyrocalcitonin, Cyclic Adenosine 3′, 5′ -Monophosphate, 25-Hydroxycholecalciferol, and Calcium Ion

The Effects of Synthetic Human and Salmon Calcitonins on Electrolyte Excretion in the Rat

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