Natriuretic activity of digoxin-like immunoreactive substance extracted from cord blood

Ebara, Hirotaka; Suzuki, Shigeyoshi; Nagashima, Kanji; Kuroume, Takayoshi

doi:10.1016/0024-3205(88)90639-x

Cited by 13 publications

(3 citation statements)

References 15 publications

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“…High plasma levels of an endogenous digoxin-like factor (EDLF) have been shown in the newborn infants [14][15][16][17], It was found to correlate inversely with gestational and post natal ages and directly with fractional sodium excretion [18]. Based on these observations it appeared to be relevant that the increased plasma EDLF is the result of the expanded extracellular fluid compartment at birth and it contributes to the disposal of neonatal salt and water excess through the kidney.…”

Section: Introductionmentioning

confidence: 93%

Urinary Excretion of Endogenous Ouabain-Like Substance Is Reduced in NaCl Supplemented Premature Infants

et al. 1997

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Ouabain or an isomer has been identified as endogenous ouabain-like substance (EOLS). The role of EOLS in the adaptation of premature infants to alterations of sodium balance was investigated by measuring urinary ouabain excretion serially in 9 low birth weight premature infants with (group S, mean birth weight 1,578 g, mean gestational age 30.4 weeks) and without (group NS, mean birth weight 1,537 g, mean gestational age 30.8 weeks) NaCl supplementation. The study was performed on the 7th day and weekly thereafter until the 5th week of life. NaCl supplementation was given in a dose of 3–5 and 1.5–2.5 mmol/kg/day at the postnatal ages of 8–21 and 22–35 days, respectively. Prior to NaCl supplementation, urinary ouabain excretion was similar in the two groups (146.2 ± 16.8 pg/kg/h in group S versus 180.0 ± 9.6 pg/kg/h in group NS) and remained at about the same level throughout the study when supplemental NaCl was provided. In infants of group NS, urinary ouabain excretion increased significantly by the 3rd week (p < 0.01) and no consistent change occurred later on. As a result, the differences in urinary ouabain excretion between the two groups proved to be significant during weeks 2–5 (p < 0.001). Essentially the same pattern of ouabain excretion was seen when it was expressed in terms of pg/mg creatinine. In infants receiving high sodium diet there was a significant positive correlation between urinary sodium and ouabain excretion. It is concluded that premature infants receiving low sodium intake have elevated EOLS excretion by the 3rd week of life. Although the relationship between ouabain and sodium excretion in supplemented premature infants suggests some physiological significance for sodium excretion, ouabain does not appear to be regulated by extracellular volume.

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Section: Introductionmentioning

confidence: 93%

Urinary Excretion of Endogenous Ouabain-Like Substance Is Reduced in NaCl Supplemented Premature Infants

et al. 1997

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“…Its role would be to maintain high sodium excretion by reducing tubular sodium reabsorption (6) in spite of the high and continuous ingestion of sodium from the amniotic fluid. Its role would be to maintain high sodium excretion by reducing tubular sodium reabsorption (6) in spite of the high and continuous ingestion of sodium from the amniotic fluid.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Rat Na⁺/K⁺-ATPase Isoforms by Endogenous Digitalis Extracts from Neonatal Human Plasma

Crambert

Balzan

Paci

et al. 1998

Clinical and Experimental Hypertension

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An unique endogenous digitalis-like factor (EDLF) has been previously purified from human newborn cord plasma and its differential effects tested on the three well defined functional isoforms (alpha1, alpha2 and alpha3) of the alpha subunits of Na+/K+-ATPase in rat. EDLF specifically inhibits the enzymatic activity. It differs from ouabain by three criteria: a preincubation with the membranes is required for full activity, no effect on the rat cerebral alpha3 isoform and a steep dose-response curve with the same apparent potency for rat alpha2 and alpha1 isoforms of high (10(-7) M) and low affinity (3 x 10(-5) M) for ouabain. These results indicate that the Na+/K+-ATPase inhibitor involved in the regulation of sodium and body fluid volume and present in neonate and adult human plasmas is distinct from ouabain.

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“…Elevated levels have been noted in volumeexpanded physiological and pathophysiological states [for review, see ref 1]. Previously, Ebara et al [4] reported the presence of a natriuretic factor in cord blood measured by 125 I-digoxin radioimmunoassay (RIA). Balzan et al [5] measured a high level of endogenous digitalis-like factor(s) in human neonate plasma using anti-ouabain antiserum.…”

Section: Introductionmentioning

confidence: 99%

Elevated Blood Levels of Endogenous Ouabain-Like Factor in Preterm versus Mature Newborns at Birth

Göõz

Vakkuri²,

Châtel³

et al. 2004

Neonatology

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Compared to healthy adults, elevated levels of endogenous ouabain-like factor (OLF) have already been shown in the cord blood, and a role of OLF in the maintenance of high Na⁺ excretion by reducing tubular sodium reabsorption during intrauterine life was suggested. In this study, we aimed to measure OLF cord blood levels of premature and mature newborns to provide further data on the possible physiological significance of this compound in neonates. OLF was assessed in the cord blood of newborns (28–41 weeks) and in the blood of healthy adults using ouabain radioimmunoassay. HPLC was employed to isolate endogenous OLF. Newborns had about twelve times higher OLF levels than healthy adults (41.96 ± 4.64 vs. 3.1 ± 1.1 pg/ml, p < 0.001). Further, there was a highly significant correlation (p < 0.004) between maturity and OLF concentration; OLF level increased with gestational age, however there was a rapid drop in its concentration at week 39 (43.26 ± 7 vs. 35.47 ± 1.84 pg/ml, p = NS). Further studies are needed to evaluate the physiological relevance of higher OLF in preterm versus mature newborns.

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Natriuretic activity of digoxin-like immunoreactive substance extracted from cord blood

Cited by 13 publications

References 15 publications

Urinary Excretion of Endogenous Ouabain-Like Substance Is Reduced in NaCl Supplemented Premature Infants

Urinary Excretion of Endogenous Ouabain-Like Substance Is Reduced in NaCl Supplemented Premature Infants

Inhibition of Rat Na⁺/K⁺-ATPase Isoforms by Endogenous Digitalis Extracts from Neonatal Human Plasma

Elevated Blood Levels of Endogenous Ouabain-Like Factor in Preterm versus Mature Newborns at Birth

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Natriuretic activity of digoxin-like immunoreactive substance extracted from cord blood

Cited by 13 publications

References 15 publications

Urinary Excretion of Endogenous Ouabain-Like Substance Is Reduced in NaCl Supplemented Premature Infants

Urinary Excretion of Endogenous Ouabain-Like Substance Is Reduced in NaCl Supplemented Premature Infants

Inhibition of Rat Na+/K+-ATPase Isoforms by Endogenous Digitalis Extracts from Neonatal Human Plasma

Elevated Blood Levels of Endogenous Ouabain-Like Factor in Preterm versus Mature Newborns at Birth

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Inhibition of Rat Na⁺/K⁺-ATPase Isoforms by Endogenous Digitalis Extracts from Neonatal Human Plasma