Native Umbilical Cord Matrix Stem Cells Express Hepatic Markers and Differentiate Into Hepatocyte-like Cells

Campard, David; Lysy, Philippe A.; Najimi, Mustapha; Sokal, Étienne

doi:10.1053/j.gastro.2007.12.024

Cited by 238 publications

(247 citation statements)

References 44 publications

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“…The human cord blood unrestricted somatic stem cells differentiation capability toward hepatocytes was shown previously (24,25). The findings of this study along with the others have been revealed that umbilical cord USSCs could express levels of hepatocyte markers (24,25).…”

Section: Discussionsupporting

confidence: 69%

“…Arvand J Health Med Sci 2016;1(1): [17][18][19][20][21][22][23][24][25][26] from hepatic or non-hepatic stem cells, are therefore considered as potential alternatives (1). In particular, mesenchymal stem cells (MSC) from cord blood (CB), adipose tissue, or bone marrow are stated as a potential stem cell source for hepatocyte or hepatocyte-like cell generation in both in vivo and in vitro approaches (2)(3)(4)(5).…”

Section: Original Article 18 Unrestricted Somatic Stem Cells Differenmentioning

confidence: 99%

“…In particular, mesenchymal stem cells (MSC) from cord blood (CB), adipose tissue, or bone marrow are stated as a potential stem cell source for hepatocyte or hepatocyte-like cell generation in both in vivo and in vitro approaches (2)(3)(4)(5). Nevertheless, studies illustrating in vitro hepatic features of stem-cell-derived hepatocyte-like cells were essentially confined to a histologic and molecular level rather than a functional approach, characterizing the hepatic-like phenotype solely by a marginal panel of markers and demonstrating simultaneous marker expression in undifferentiated nonhepatic cells.…”

Section: Original Article 18 Unrestricted Somatic Stem Cells Differenmentioning

confidence: 99%

“…Surface marker analysis by flow cytometry showed that the cells were positive for CD105 (98%), CD90 (97%), CD166 (86%), and CD44 (99%) but were negative for CD34 (0.004) and CD45 (0.005%) (Figure 2). More extensive characterization of these cells is available in our previous publications (1)(2)(3)(4)(5).…”

Section: Periodic Acid-schiff Staining (Pas) For Glycogenmentioning

confidence: 99%

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Unrestricted somatic stem cells differentiation into hepatic-like cells in a three dimensional arrangement

Tavirani

Keshel

Takcalou

2016

Arvand Journal of Health and Medical Sciences

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Reports describing the human cord blood derived mesenchymal stem cells (hUSSCs) hepatocytic differentiation potential are rare and there is no report describing fibrin base 3D culture of hepatocyte-like cells differentiated from cord blood unrestricted somatic stem cells. Hepatocyte-like differentiated stem cells has the potential to provide compensation for acute liver failure patients. Therefore, we characterized cord blood Unrestricted Somatic Stem Cells (USSC) and their hepatocyte differentiation capabilities for functional liver support in a three dimensional arrangement. Moreover, three-dimensional (3D) network and its distribution to grow and differentiate stem cells in so far as possible mimic to native tissue holds huge potential in liver tissue engineering. In this study, we isolate USSCs from human cord blood and validate them with flow-cytometry. Briefly, we differentiated cells using DMEM containing FGF4, Activin A, HGF and 2% FBS into hepatocyte-like cells (HLCs) in two conditions; 3D state in biocompatible fibrin Hydrogel and or conventional culture (2D) in polystyrene plates for 21 days. Immuncytochemical and gene expression analysis revealed the expression of KRT18, KRT19, albumin, Sox17, FoxA2 and Prox1 increased in 3D fibrin culture compared to 2D conventional culture. In combination, these data reveal that using 3D networks can resemble near natural tissue properties for effective generating HLCs which used to cell replacement in the future. As well as, USSC represent a stem cell source with a substantial hepatic differentiation capability which hold the possible for clinical applications.

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Section: Discussionsupporting

confidence: 69%

Section: Original Article 18 Unrestricted Somatic Stem Cells Differenmentioning

confidence: 99%

Section: Original Article 18 Unrestricted Somatic Stem Cells Differenmentioning

confidence: 99%

Section: Periodic Acid-schiff Staining (Pas) For Glycogenmentioning

confidence: 99%

See 2 more Smart Citations

Unrestricted somatic stem cells differentiation into hepatic-like cells in a three dimensional arrangement

Tavirani

Keshel

Takcalou

2016

Arvand Journal of Health and Medical Sciences

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“…2,3 In particular, MSCs can differentiate into hepatocyte-like cells through a specific cell-signaling pathway upon stimulation with a combination of hepatocyte growth factor (HGF), fibroblast growth factor, epidermal growth factor and other cytokines. [4][5][6] However, in a culture of human bone marrowderived MSCs (BM-MSCs) and hepatocytes, BM-MSCs that undergo hepatic differentiation probably account for only a small percentage of the population, 7 and any differentiation may not significantly change the outcome of the bone marrow stromal cell (BMSC) and hepatocyte coculture experiments. 8 MSCs have recently emerged as promising candidates for cell-based immunotherapy because these cells can modulate the immune responses in various ways.…”

Section: Therapeutic Mechanism Of Msc Transplantationmentioning

confidence: 99%

Implications of the immunoregulatory functions of mesenchymal stem cells in the treatment of human liver diseases

Zhang

et al. 2010

Cell Mol Immunol

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Transplantation of mesenchymal stem cells (MSCs) has been recently studied in animal models, and in clinical trials of patients with fulminant hepatic failure, end-stage liver diseases and inherited metabolic disorders. Modulatory cytokines produced by MSCs can inhibit immunocyte proliferation and migration to the liver, thereby attenuating inflammatory injury and reducing hepatocyte apoptosis. In addition, MSCs play an important role in regressing liver fibrosis and in supporting the function, proliferation and differentiation of endogenous hepatocytes under appropriate conditions. Although remarkable progress has been achieved in basic and clinical MSC studies, optimal therapeutic regimens for the clinical application of MSCs, such as optimal doses, transplantation routine and interval period for transplantation, need to be elucidated in detail. Furthermore, the long-term safety and therapeutic efficacy of MSC transplantation should be evaluated in future clinical trials. This review summarizes our current understanding of the immunomodulatory effects of MSC therapies on human liver diseases.

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Human foreskin fibroblast‐like stromal cells can differentiate into functional hepatocytic cells

Huang

Chen

Wang

et al. 2013

Cell Biology International

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Foreskin fibroblast-like stromal cells (FDSCs) are progenitors isolated from human tissue that can differentiate into diverse cell types. Many types of stem cells can differentiate into hepatocyte-like cells, which could be used for drug testing or in liver regeneration therapy, but whether FDSCs can be converted into functional hepatocytes is unknown. FDSCs show divergent properties when cultured in distinct media, forming spheres in Dulbecco's modified Eagle's medium (DMEM) containing F12, epidermal growth factor (EGF), and basic fibroblast growth factor (b-FGF), but have fibroblast-like morphology when cultured in DMEM-based growth medium. Both cell populations express the typical mesenchymal stem cell markers CD90, CD105, and CD73, but the p75 neurotrophin receptor (p75NTR) was detected only in FDSC spheres. Both types of FDSCs can differentiate into hepatocyte-like cells, which express typical liver markers, including albumin and hepatocyte paraffin 1 (Hep Par1), along with liver-specific biological activities. When plasmids containing the human hepatitis B virus (HBV) genome were transfected transiently into FDSCs, differentiated hepatocyte-like cells secrete large amounts of HBe and HBs antigens. FDSCs could be used for clinical hepatic therapy and/or serve as a model of HBV.

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Native Umbilical Cord Matrix Stem Cells Express Hepatic Markers and Differentiate Into Hepatocyte-like Cells

Cited by 238 publications

References 44 publications

Unrestricted somatic stem cells differentiation into hepatic-like cells in a three dimensional arrangement

Unrestricted somatic stem cells differentiation into hepatic-like cells in a three dimensional arrangement

Implications of the immunoregulatory functions of mesenchymal stem cells in the treatment of human liver diseases

Human foreskin fibroblast‐like stromal cells can differentiate into functional hepatocytic cells

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