Native Spleen Preservation During Visceral Transplantation Inhibits Graft-Versus-Host-Disease Development

Stringa, Pablo; Papa‐Gobbi, Rodrigo; Vela, María; Gentilini, M; Machuca, Mariana Alejandra; Klin, Pablo; Arreola, Nidia Monserrat; Serradilla, Javier; Bueno, Alba; Andrés, Ane Miren; Ramos, Esther; Alcolea, Alida; Pérez‐Martínez, Antonio; Prieto, Gerardo; López‐Santamaría, Manuel; Gondolesi, Gabriel; Rumbo, Martı́n; Hernández, Francisco Félix Clavijo

doi:10.1097/sla.0000000000004979

Cited by 7 publications

(7 citation statements)

References 27 publications

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“…Epithelial cells isolation from ileum from chickens. Single cell suspensions of 10 centimeters of ileum were prepared as described previously 42 . Briefly, intestinal sections from the ileum were rinsed, cut longitudinally and washed with cold PBS containing 1% antibiotics.…”

Section: Methodsmentioning

confidence: 99%

Few amino acid mutations in H6 influenza A virus from South American lineage increase pathogenicity in poultry

Rimondi

Olivera

Soria

et al. 2022

Preprint

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In chickens, infections with influenza A virus (IAV) can range from mild to severe and lethal. The study of IAV infections in poultry has been mostly limited to strains from the North American and Eurasian lineages, whereas limited information exists on similar studies with strains from the South American lineage (SAm). To better evaluate the risk of introduction of a prototypical SAm IAV strain into poultry, chickens were infected with a wild-type SAm origin strain (WT557/H6N2). The resulting virus progeny was serially passage in chickens 20 times and the immunopathological effects in chickens of the last passage virus, 20Ch557/H6N2, was compared to those of the parental strain. Comparison of complete viral genome sequences indicated that the 20Ch557/H6N2 strain contained 13 amino acid differences compared to the wild type strain. Five of these mutations are in functionally relevant regions of the viral surface glycoproteins hemagglutinin (HA) and neuraminidase (NA). However, despite higher and more prolonged virus shedding in chickens inoculated with the 20Ch557/H6N2 strain compared to those that received the WT557/H6N2 strain, transmission to naïve chickens was not observed for either group. Analyses by flow cytometry of mononuclear cells and lymphocyte subpopulations from the lamina propria and intraepithelial lymphocytic cells (IELs) from ileum revealed significant increase in the percentages of CD3+TCRγδ+ IELs in chickens inoculated with the 20Ch557/H6N2 strain compared to those inoculated with the WT557/H6N2 strain.

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Section: Methodsmentioning

confidence: 99%

Few amino acid mutations in H6 influenza A virus from South American lineage increase pathogenicity in poultry

Rimondi

Olivera

Soria

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Single cell suspensions of 10 centimeters of ileum were prepared as described previously ( Stringa et al, 2021 ). Briefly, intestinal sections from the ileum were rinsed, cut longitudinally, and washed with cold PBS containing 1% antibiotics.…”

Section: Methodsmentioning

confidence: 99%

Few Amino Acid Mutations in H6 Influenza A Virus From South American Lineage Increase Viral Replication Efficiency in Poultry

et al. 2022

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In chickens, infections due to influenza A virus (IAV) can be mild to severe and lethal. The study of IAV infections in poultry has been mostly limited to strains from the North American and Eurasian lineages, whereas limited information exists on similar studies with strains from the South American lineage (SAm). To better evaluate the risk of introduction of a prototypical SAm IAV strain into poultry, chickens were infected with a wild-type SAm origin strain (WT557/H6N2). The resulting virus progeny was serially passaged in chickens 20 times, and the immunopathological effects of the last passage virus, 20Ch557/H6N2, in chickens were compared to those of the parental strain. A comparison of complete viral genome sequences indicated that the 20Ch557/H6N2 strain contained 13 amino acid differences compared to the wild-type strain. Five of these mutations are in functionally relevant regions of the viral surface glycoproteins hemagglutinin (HA) and neuraminidase (NA). However, despite higher and more prolonged virus shedding in chickens inoculated with the 20Ch557/H6N2 strain compared to those that received the WT557/H6N2 strain, transmission to naïve chickens was not observed for either group. Analyses by flow cytometry of mononuclear cells and lymphocyte subpopulations from the lamina propria and intraepithelial lymphocytic cells (IELs) from the ileum revealed a significant increase in the percentages of CD3+TCRγδ+ IELs in chickens inoculated with the 20Ch557/H6N2 strain compared to those inoculated with the WT557/H6N2 strain.

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“…The underlying mechanism may involve the clearance of donor lymphoid cells by native spleen macrophages. 76 Given this evidence, it would be reasonable to consider native spleen preservation as a potential strategy to reduce the risk of GvHD in patients undergoing MVTx. Although native spleen preservation may be beneficial in reducing the risk of GvHD, further investigations are warranted to fully study the mechanisms involved and to determine the optimal approaches for applying this surgical strategy in clinical practice.…”

Section: Current Operative and Pharmacological Approaches To Preventi...mentioning

confidence: 99%

Current Advances in Graft-versus-host Disease After Intestinal Transplantation

et al. 2024

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Graft-versus-host disease (GvHD) remains a potentially fatal complication following intestinal transplant (ITx). Over the past decade, advances in the understanding of the pathophysiology of this complex immunological phenomenon have led to the reassessment of the host systemic immune response and have created a gateway for novel preventive and therapeutic strategies. Although sufficient evidence dictates the use of corticosteroids as a first-line option, the treatment for refractory disease remains contentious and lacks a standardized therapeutic approach. Timely diagnosis remains crucial, and the advent of chimerism detection and immunological biomarkers have transformed the identification, prognostication, and potential for survival after GvHD in ITx. The objectives of the following review aim to discuss the clinical and diagnostic features, pathophysiology, advances in immune biomarkers, as well as therapeutic opportunities in the prevention and treatment of GvHD in ITx.

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Native Spleen Preservation During Visceral Transplantation Inhibits Graft-Versus-Host-Disease Development

Cited by 7 publications

References 27 publications

Few amino acid mutations in H6 influenza A virus from South American lineage increase pathogenicity in poultry

Few amino acid mutations in H6 influenza A virus from South American lineage increase pathogenicity in poultry

Few Amino Acid Mutations in H6 Influenza A Virus From South American Lineage Increase Viral Replication Efficiency in Poultry

Current Advances in Graft-versus-host Disease After Intestinal Transplantation

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