Native SAD is maturing

Rose, John P.; Wang, Bi-Cheng; Weiss, M.S.

doi:10.1107/s2052252515008337

Cited by 52 publications

(62 citation statements)

References 63 publications

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“…For MDS approaches [97][98][99][100], the Garman limit for absorbed radiation dose [101] is determined. The resulting exposure time is then "dose-sliced" by dividing the exposure time to achieve the Garman limit by the number of data sets to be collected.…”

Section: Phasing and Data Collectionmentioning

confidence: 99%

“…Copper is by far the most common source available for in-house data collection. The use of chromium targets has been thoroughly investigated and proven useful [97,98] although the increase in air-scatter generally requires the additional complication of a helium filled chamber between the crystal and detector face to maintain an acceptable signal:noise ratio.…”

Section: Choice Of Wavelengthmentioning

confidence: 99%

See 1 more Smart Citation

Phosphorus SAD Phasing for Nucleic Acid Structures: Limitations and Potential

2016

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Phasing of nucleic acid crystal diffraction data using the anomalous signal of phosphorus, P-SAD, at Cuk α wavelength has been previously demonstrated using Z-DNA. Since the original work on P-SAD with Z-DNA there has been, with a notable exception, a conspicuous absence of applications of the technique to additional nucleic acid crystal structures. We have reproduced the P-SAD phasing of Z-DNA using a rotating-anode source and have attempted to phase a variety of nucleic acid crystals using P-SAD without success. A comparison of P-SAD using Z-DNA and a representative nucleic acid, the Dickerson-Drew dodecamer, is presented along with a S-SAD using only two sulfurs to phase a 2'-thio modified DNA decamer. A theoretical explanation for the limitation of P-SAD applied to nucleic acids is presented to show that the relatively high atomic displacement parameter of phosphorus in the nucleic acid backbone is responsible for the lack of success in applying P-SAD to nucleic acid diffraction data.

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Section: Phasing and Data Collectionmentioning

confidence: 99%

Section: Choice Of Wavelengthmentioning

confidence: 99%

Phosphorus SAD Phasing for Nucleic Acid Structures: Limitations and Potential

2016

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“…The design of MX beamlines at synchrotrons has been optimized over the past years towards fully automated highthroughput instruments for wavelengths around the Se K edge ( = 0.979 Å ). Many of these beamlines can access wavelengths up to 2.3 Å and most of the work cited above has been successfully performed in this regime (Rose et al, 2015). Beamline BL-1A at the Photon Factory can access wavelengths up to 3.5 Å and the first successful results have been published (Ru et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Many of these beamlines can access wavelengths up to 2.3 Å and most of the work cited above has been successfully performed in this regime (Rose et al, 2015). Beamline BL-1A at the Photon Factory can access wavelengths up to 3.5 Å and the first successful results have been published (Ru et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

In-vacuum long-wavelength macromolecular crystallography

Wagner

Duman

Henderson

et al. 2016

Acta Crystallogr D Struct Biol

101

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Structure solution based on the weak anomalous signal from native (protein and DNA) crystals is increasingly being attempted as part of synchrotron experiments. Maximizing the measurable anomalous signal by collecting diffraction data at longer wavelengths presents a series of technical challenges caused by the increased absorption of X-rays and larger diffraction angles. A new beamline at Diamond Light Source has been built specifically for collecting data at wavelengths beyond the capability of other synchrotron macromolecular crystallography beamlines. Here, the theoretical considerations in support of the long-wavelength beamline are outlined and the in-vacuum design of the endstation is discussed, as well as other hardware features aimed at enhancing the accuracy of the diffraction data. The first commissioning results, representing the first in-vacuum protein structure solution, demonstrate the promising potential of the beamline.

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“…Over the past years technical developments such as single-photon counting detectors and improved stability from synchrotron sources and beamline equipment have led to an increased number of protein and nucleic acid structures being solved by experimental phasing techniques at longer wavelengths around λ = 2 Å [1]. Single wavelength anomalous diffraction (SAD) utilizes therein the increase of the anomalous signal from sulfur or phosphorus towards their K absorption edges which are around 5 Å and 6 Å, respectively.…”

mentioning

confidence: 99%