Native polyubiquitin promoter of rice provides increased constitutive expression in stable transgenic rice plants

Abstract

The rice Ubiquitin1 (Ubi1) promoter was tested to evaluate its capacity to express the heterologous gusA gene encoding β-glucuronidase in transgenic rice tissue relative to the commonly used Ubi1 corn promoter and the rice gibberellic acid insensitive (GAI) gene promoter element. Experimental results showed increased expression of gusA gene in rice tissue when driven by the native Ubi1 promoter when compared to the use of corn Ubi1 promoter. Results further indicated that the cis-regulatory elements present in the native promoter element might have been responsible for high expression. However, the gusA gene expression level when driven by the rice GAI promoter was notably lower than both Ubi1 promoters. The present study, thus, for the first time helped to demonstrate that the native Ubi1 promoter is a promising genetic element in transgenic approaches for constitutive expression of any gene in rice tissue.

A deletion mutant ndv200 of the Bacillus thuringiensis vip3BR insecticidal toxin gene is a prospective candidate for the next generation of genetically modified crop plants resistant to lepidopteran insect damage

“…Western blot analysis was performed following the protocol described earlier (Bhattacharyya et al 2012). Proteins extracted from homogenized leaf tissue of transgenic lines extracted in buffer (20 mM Tris, 100 mM NaCl, 0.02 % Tween20, and 10 mM PMSF).…”

Stable Transformation of Ferns Using Spores as Targets: Pteris vittata and Ceratopteris thalictroides

“…Selection regimes can be fine-tuned and/or a promoter stronger than nopaline synthase (nos) is likely required. Because the nos promoter is not a strong constitutive promoter, even in angiosperms (Christensen and Quail, 1996;Wilkinson et al, 1997;Bhattacharyya et al, 2012), the next likely step would be to replace it with a stronger promoter in the selectable marker cassette. We noted that young prothalli are sensitive to hygromycin; lower amounts of hygromycin might be required if antibiotic selection is desired.…”

Characterization of a small constitutive promoter from Arabidopsis translationally controlled tumor protein (AtTCTP) gene for plant transformation

“…So far, a limited number of constitutive promoters have been used to direct transgene expression in plants, and they are often derived from non-plant sources such as the cauliflower mosaic virus (CaMV) 35S promoter (Benfey and Chua 1990;Odell et al 1985), cassava vein mosaic virus (CsVMV) promoter (Verdaguer et al 1996), and nopaline synthase gene (NOS) promoter from Agrobacterium tumefaciens (Bevan et al 1983). On the other hand, constitutive promoters of plant genes have generally not been studied extensively (Bhattacharyya et al 2012), and the widely used 35S promoter was known to be less efficient in some plant species (Christensen et al 1992;Park et al 2010). Thus, plantderived constitutive promoters can be used as an alternative to the 35S promoter.…”