Native mass spectrometry reveals binding interactions of SARS-CoV-2 PLpro with inhibitors and ISG15
Virginia James,
Rianna Godula,
Jessica Perez
et al.
Abstract:Here we used native mass spectrometry (native MS) to probe a SARS-CoV protease, PLpro, which plays critical roles in coronavirus disease by affecting viral protein production and antagonizing host antiviral responses. Ultraviolet photodissociation (UVPD) and variable temperature electrospray ionization (vT ESI) were used to localize binding sites of PLpro inhibitors and revealed the stabilizing effects of inhibitors on protein tertiary structure. We compared PLpro from SARS-CoV-1 and SARS-CoV-2 in terms of inh… Show more
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