Native Macrofungi that Produce Lignin-Modifying Enzymes, Cellulases, and Xylanases with Potential Biotechnological Applications

Abstract

With the aim of identifying and exploiting the mycological resources available in the Mexican Sierra Madre Oriental, the lignocellulolytic and pectinolytic potential of autochthonous fungi were evaluated in the present work. A solid media selection system was established in which 74 isolated strains were tested and compared to six international reference strains. The macrofungi Xylaria sp CS121, Inonotus sp CU7, Basidiomycete CH32, Basidiomycete CH23, Xylaria poitei, and Trametes maxima CU1 showed the highest cellulolytic and pectinolytic potential. The greatest lignolytic capability was exhibited by T. maxima CU1 and Pycnoporus sanguineus CS43. Under stirred submerged culture, T. maxima CU1 (cellulases, cellobiose dehydrogenase, manganese peroxidase (MnP), and laccase, with 200, 359, 51, and 267 U/L, respectively) and Xylaria sp CS121 (198 U/L of xylanases) were the highest enzymatic producers. Under stationary conditions, the best producers were Inonotus sp CU7 for cellulases, P. sanguineus CS43 for cellobiose dehydrogenase and laccase, and T. maxima CU1 for xylanases and MnP (242, 467, 35, 165, and 31 U/L, respectively). These results demonstrate the efficiency of enzymatic profiling as a tool for enzyme discovery with Mexican native fungi.

Biodegradation of Residues from the Palo Santo (Bursera graveolens) Essential Oil Extraction and Their Potential for Enzyme Production Using Native Xylaria Fungi from Southern Ecuador

“…are potential producers of cellulolytic enzymes, due to their high cellulolytic activity, and therefore can be used in biotechnological applications as well as for industrial purposes. This is confirmed by Gutiérrez-Soto et al [16], who measured the cellulase activity of Xylaria spp. up to 199 U/L.…”

“…Xylaria spp. are considered to be significant producers of different ligninolytic enzymes, including laccase, xylanase and cellulase [16], besides their ability to degrade lignin [17], hemicellulose [18], and cellulose from trees and agricultural residues [19][20][21][22]. Within the ecosystems, this type of fungus participates in the carbon and nitrogen cycles [23] and plays an important role in the biodegradation processes of wood and leaf litter due to its complex and diverse enzymatic system.…”

The effects of hydrolysed sorghum on growth performance and meat quality of rabbits

“…Trametes maxima CU1 and Pycnoporus sanguineus CS2 are 2 basidiomycetes from North-eastern Mexico with great lignocellulose potential (Hernández-Luna et al, 2008;Gutiérrez-Soto et al, 2015a), and are recognised for their production of laccases with high redox potential, as well as for the production of thermostable cellulases, xylanases and pectinases (Gutiérrez-Soto et al,2015b). The aim of the current study was to evaluate the potential use of sorghum hydrolysed by Trametes maxima CU1 and Pycnoporus sanguineus CS2 strains as a feed supplement to enhance rabbit performance and meat quality.…”

Evaluation of the mycelial growth of Trametes villosa (Sw.) Kreisel in media supplemented with copper (II) and sugarcane vinasse

“…Esta información es fundamental para el inicio del desarrollo de protocolos a nivel de laboratorio en los que se aprovechen las capacidades bioquímicas de estos organismos. El crecimiento micelial medido como área o diámetro en función del tiempo, es una de las variables de respuesta macroscópica utilizada en estudios de adaptación in vitro (Lee et al 2014, Gutiérrez-Soto et al 2015, Martínez et al 2015. A partir de esta variable primaria, se obtienen características de crecimiento secundarias como es el caso de la velocidad específica de crecimiento (µ) (Panikov 2011), el cual es un parámetro combinado que abarca las distintas etapas de la morfogénesis micelial (Viniegra- González et al 1993).…”