“…Comparison of the chemical shift values with literature data allowed the identification of two minor components: protoanemonin ( δ 13 C NMR: 170.11 ppm (C=O), 154.92 ppm (C), 143.49 ppm (CH), 121.72 ppm (CH), and 98.31 ppm (CH 2 )) and anemonin ( δ 13 C NMR: 170.11 ppm (C=O), 153.57 ppm (CH), 121.06 ppm (CH), 90.41 ppm (C), and 23.82 ppm (CH 2 )). The NMR data of these two compounds fit with those reported by Southwell and Tucker [ 11 ], and Kern and Cardellina [ 12 ]. Protoanemonin, an irritant lactone, and its dimerization product, anemonin, are characteristic constituents of Ranunculaceae species such as H. Niger [ 13 ] or H. foetidus [ 14 ].…”