National BioResource Project-Rat and Related Activities

Serikawa, Tadao; Mashimo, Tomoji; Takizawa, Akiko; Okajima, Ryoko; Maedomari, Naoki; Kumafuji, Kenta; Tagami, Fumi; Neoda, Yuki; Otsuki, Mito; Nakanishi, Satoshi; Yamasaki, Kenichi; Voigt, Birger; Kuramoto, Takashi

doi:10.1538/expanim.58.333

Cited by 65 publications

(62 citation statements)

References 27 publications

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“…The laboratory rat (Rattus norvegicus) has been widely used as an animal model of human diseases, because its size is suitable for manipulation [1,27]. Sequencing of the rat genome has shown that the rat has about 20,000 predicted genes and shares as many as 90% with humans [9].…”

Section: Introductionmentioning

confidence: 99%

Genetic Analyses of Fancy Rat-Derived Mutations

et al. 2010

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Abstract:To collect rat mutations and increase the value of the rat model system, we introduced fancy-derived mutations to the laboratory and carried out genetic analyses. Six fancy rats were shipped from a fancy rat colony in the USA and used as founders. After initial crosses with a laboratory strain, TM/Kyo or PVG/Seac, inbreeding started and 6 partially inbred lines, including 2 sublines, were produced as Kyoto Fancy Rat Stock (KFRS) strains. During inbreeding, we isolated 9 mutations: 5 coat colors, American mink (am), Black eye (Be), grey (g), Pearl (Pel), siamese (sia); 1 coat pattern, head spot (hs); 2 coat textures, Rex (Re), satin (sat); and an ear pinnae malformation, dumbo (dmbo). Genetic analyses mapped 7 mutations to particular regions of the rat chromosomes (Chr): am to Chr 1, sia to Chr 1, sat to Chr 3, Re to Chr 7, g to Chr 8, dmbo to Chr 14, and hs to Chr 15. Candidate gene analysis revealed that a missense mutation in the tyrosinase gene, Ser79Pro, was responsible for sia. From mutant phenotypes and mapping positions, it is likely that all mutations isolated in this study were unique to the fancy rat. These findings suggest that fancy rat colonies are a good source for collecting rat mutations. The fancy-derived mutations, made available to biomedical research in the current study, will increase the scientific value of laboratory rats.

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Section: Introductionmentioning

confidence: 99%

Genetic Analyses of Fancy Rat-Derived Mutations

et al. 2010

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“…The laboratory rat (Rattus norvegicus) has been widely used as an animal model of human diseases, because its size is suitable for manipulation [1,12]. The rat model system is also used for understanding gene function.…”

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confidence: 99%

“…KFRS5A/Kyo strains were obtained from the National BioResource Project -Rat (NBRP-Rat) [12]. For histopathology, dorsal skins were collected from Re/Re, Re/+, and wild-type (+/+) rats, fixed in 10% buffered formalin, and embedded in paraffin.…”

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confidence: 99%

Identification of the Rat Rex Mutation as a 7-bp Deletion at Splicing Acceptor Site of the Krt71 Gene

et al. 2010

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ABSTRACT. The rat autosomal dominant Rex (Re) mutation on chromosome 7 causes curly hair in Re/+ and hair loss in Re/Re rats. Histopathologically, the Re/+ rat showed dilatation of the hair follicle and hairs with irregularly-coated cuticles, and the Re/Re rat showed more severe effects. We identified Re as a 7-bp deletion at the splicing acceptor site of intron 1 of the keratin 71 (Krt71) gene, which is located within the Re critical chromosomal region and plays an important role in hair formation. The deletion provoked a 6-amino acid in-frame deletion (p.Val149_Gln154del) in the -helical rod domain of KRT71 protein. Identification of the Re mutation (Krt71 Re ) enables us to further understand the biological function of KRT71.

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“…The tracer was delivered into proximal dorsal tail vein using a Model NE-1000 syringe pump (New Era Syringe Pumps, Farmingdale, NY) via 0. ( 16 ). The tracer infusion had no effect on the level of plasma FFA.…”

Section: Isotope Infusion and Tissue Collectionmentioning

confidence: 87%

Intramyocellular diacylglycerol concentrations and [U-13C]palmitate isotopic enrichment measured by LC/MS/MS

Błachnio-Zabielska

Jensen

2013

Journal of Lipid Research

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Recently, more attention has been focused on DAG because of their purported role to induce muscle and liver insulin resistance when present in excess ( 1-4 ). Because of the biological functions of DAG, especially DAG species containing saturated fatty acids, we endeavored to develop a method to precisely and rapidly measure muscle DAG.Most methods for DAG measurement quantify total DAG content. One of the fi rst and common quantitative analysis of DAG was performed by enzymatic analysis using diacylglycerol kinase assay ( 5 ) using labeled ATP 32 . A nonradioactive technique for total DAG quantifi cation uses HPLC analysis following derivatization ( 6 ). The composition of DAG fatty acids can be determined by hydrolysis of DAG and analysis of the liberated and derivatized fatty acid by the means of gas-liquid chromatography with fl ame ionization detector (GLC-FID). Typically, DAG are separated from an extract of total lipids using thin-layer chromatography (TLC) or HPLC. TLC separation requires long processing times, leads to loss of analyte, and is susceptible to sample contamination. Development of mass spectrometry methods has facilitated analysis of DAG by reducing the analysis time, the amounts of consumables, and the likelihood of sample contamination. Diacylglycerols and triacylglycerols are classes of neutral lipids without any permanent charge; therefore, they are usually analyzed by ESI/MS as a sodium [M + Na] + , lithium [M + Li] + , or ammonium adduct that is created spontaneously by adding the appropriate salt to the solvent system ( 7, 8 ) or after the derivatization procedure ( 9, 10 ).Because the potential contribution of circulating FFA to DAG synthesis is unknown, we developed a new method for measuring both concentration and isotopic enrichment of DAG in tissue from animals infused intravenously with uniformly labeled [U-13 C]palmitate. Our method displays excellent inter-and intraassay reproducibility, low

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National BioResource Project-Rat and Related Activities

Cited by 65 publications

References 27 publications

Genetic Analyses of Fancy Rat-Derived Mutations

Genetic Analyses of Fancy Rat-Derived Mutations

Identification of the Rat Rex Mutation as a 7-bp Deletion at Splicing Acceptor Site of the Krt71 Gene

Intramyocellular diacylglycerol concentrations and [U-13C]palmitate isotopic enrichment measured by LC/MS/MS

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