Nation-wide study of the occurrence of Listeria monocytogenes in French soils using culture-based and molecular detection methods

Locatelli, Aude; Depret, Géraldine; Jolivet, Claudy; Henry, Sonia; Dequiedt, Samuel; Piveteau, Pascal; Hartmann, Alain

doi:10.1016/j.mimet.2013.03.017

Cited by 30 publications

(33 citation statements)

References 32 publications

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“…Other researchers detected higher L. monocytogenes isolation rates (7 to 17%) in the natural environment (25,26,45). Many factors were assumed to contribute to a biased Listeria detection.…”

Section: Discussionmentioning

confidence: 94%

“…These include fewer freezing and thawing cycles before sampling, proximity to water, higher water storage capacities of soil, a high percentage of clay in the soil texture, the absence of endogenous soil microbiota, and the presence of protozoa and nematodes as vectors (23,26,43). A recent study indicated, controversially, shorter survival capacities of L. monocytogenes in soil models with high clay content (45). Listeria persistence in soil was also assumed to be facilitated by strain motility and low temperatures (Ͻ8°C) (31).…”

Section: Discussionmentioning

confidence: 99%

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Reservoirs of Listeria Species in Three Environmental Ecosystems

Linke

Rückerl

Brugger

et al. 2014

Appl Environ Microbiol

162

182

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Soil and water are suggested to represent pivotal niches for the transmission of Listeria monocytogenes to plant material, animals, and the food chain. In the present study, 467 soil and 68 water samples were collected in 12 distinct geological and ecological sites in Austria from 2007 to 2009. Listeria was present in 30% and 26% of the investigated soil and water samples, respectively. Generally, the most dominant species in soil and water samples were Listeria seeligeri, L. innocua, and L. ivanovii. The human-and animal-pathogenic L. monocytogenes was isolated exclusively from 6% soil samples in regions A (mountainous region) and B (meadow). Distinct ecological preferences were observed for L. seeligeri and L. ivanovii, which were more often isolated from wildlife reserve region C (Lake Neusiedl) and from sites in proximity to wild and domestic ruminants (region A). The higher L. monocytogenes detection and antibiotic resistance rates in regions A and B could be explained by the proximity to agricultural land and urban environment. L. monocytogenes multilocus sequence typing corroborated this evidence since sequence type 37 (ST37), ST91, ST101, and ST517 were repeatedly isolated from regions A and B over several months. A higher L. monocytogenes detection and strain variability was observed during flooding of the river Schwarza (region A) and Danube (region B) in September 2007, indicating dispersion via watercourses.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Reservoirs of Listeria Species in Three Environmental Ecosystems

Linke

Rückerl

Brugger

et al. 2014

Appl Environ Microbiol

162

182

View full text Add to dashboard Cite

show abstract

“…It has been isolated from water systems (De Luca et al, 1998; Paillard et al, 2005; Lyautey et al, 2007), vegetation (Welshimer, 1968; Beuchat, 1996), farms (Nightingale et al, 2004; Fox et al, 2009; Latorre et al, 2010; Strawn et al, 2013), food industries (Goulet et al, 1998; Garrido et al, 2009; Serio et al, 2011), and feces of animals (Fenlon, 1985; Iida et al, 1991). It is also found in soil (Welshimer, 1960; Weis and Seeliger, 1975; Locatelli et al, 2013a; Vivant et al, 2013a). A communication system has been characterized in this organism.…”

Section: Introductionmentioning

confidence: 98%

The Agr communication system provides a benefit to the populations of Listeria monocytogenes in soil

Vivant

Garmyn

Gal

et al. 2014

Front. Cell. Infect. Microbiol.

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In this study, we investigated whether the Agr communication system of the pathogenic bacterium Listeria monocytogenes was involved in adaptation and competitiveness in soil. Alteration of the ability to communicate, either by deletion of the gene coding the response regulator AgrA (response-negative mutant) or the signal pro-peptide AgrD (signal-negative mutant), did not affect population dynamics in soil that had been sterilized but survival was altered in biotic soil suggesting that the Agr system of L. monocytogenes was involved to face the complex soil biotic environment. This was confirmed by a set of co-incubation experiments. The fitness of the response-negative mutant was lower either in the presence or absence of the parental strain but the fitness of the signal-negative mutant depended on the strain with which it was co-incubated. The survival of the signal-negative mutant was higher when co-cultured with the parental strain than when co-cultured with the response-negative mutant. These results showed that the ability to respond to Agr communication provided a benefit to listerial cells to compete. These results might also indicate that in soil, the Agr system controls private goods rather than public goods.

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“…The pathogen is largely spread in the environment. It has been isolated from water systems (2-4), vegetation (5), soil (6)(7)(8), farms (9)(10)(11)(12), food industries (13)(14)(15), and the feces of animals (16)(17)(18). Environmental adaptation requires that the cell have the ability to integrate environmental cues in order to adapt its physiology to the surrounding conditions through the regulation of gene expression.…”

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confidence: 99%

Survival of Listeria monocytogenes in Soil Requires AgrA-Mediated Regulation

Vivant

Garmyn

Gal

et al. 2015

Appl Environ Microbiol

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In this study, we investigated whether the Agr-mediated response is triggered during adaptation in soil, and we compared survival patterns in a set of 10 soils. The fate of the parental strain L. monocytogenes L9 (a rifampin-resistant mutant of L. monocytogenes EGD-e) and that of a ⌬agrA deletion mutant were compared in a collection of 10 soil microcosms. The ⌬agrA mutant displayed significantly reduced survival in these biotic soil microcosms, and differential transcriptome analyses showed large alterations of the transcriptome when AgrA was not functional, while the variations in the transcriptomes between the wild type and the ⌬agrA deletion mutant were modest under abiotic conditions. Indeed, in biotic soil environments, 578 protein-coding genes and an extensive repertoire of noncoding RNAs (ncRNAs) were differentially transcribed. The transcription of genes coding for proteins involved in cell envelope and cellular processes, including the phosphotransferase system and ABC transporters, and proteins involved in resistance to antimicrobial peptides was affected. Under sterilized soil conditions, the differences were limited to 86 genes and 29 ncRNAs. These results suggest that the response regulator AgrA of the Agr communication system plays important roles during the saprophytic life of L. monocytogenes in soil. Listeria monocytogenes is the causative agent of listeriosis, a serious foodborne infection affecting essentially immunocompromised individuals, the elderly, and pregnant women (1). The pathogen is largely spread in the environment. It has been isolated from water systems (2-4), vegetation (5), soil (6-8), farms (9-12), food industries (13-15), and the feces of animals (16-18). Environmental adaptation requires that the cell have the ability to integrate environmental cues in order to adapt its physiology to the surrounding conditions through the regulation of gene expression. Genomics showed that an important part of the L. monocytogenes genome (7.3%) is dedicated to regulation and includes 209 transcriptional regulators, 15 histidine kinases, and 16 response regulators constituting two-component systems (19). Two-component systems participate in the ability of bacteria to sense and respond to fluctuating environmental conditions. AgrC/AgrA is a two-component regulatory system that is part of the Agr communication system. Initially described in Staphylococcus aureus, this communication system is organized as a four-gene operon, agrBDCA. AgrB is a membrane-bound protein that processes the propeptide AgrD into a mature autoinducing peptide (AIP). Detection of AIP by the histidine kinase AgrC induces transcriptional regulation through activation of the regulator AgrA. Detailed data concerning the role of the Agr system in the physiology of S. aureus are available (20)(21)(22). So far, its role in the adaptation of L. monocytogenes to its environment is only partially understood (23). Reports show that the Agr communication system of L. monocytogenes is involved in adhesion to abiotic surfaces (24) in ...

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Nation-wide study of the occurrence of Listeria monocytogenes in French soils using culture-based and molecular detection methods

Cited by 30 publications

References 32 publications

Reservoirs of Listeria Species in Three Environmental Ecosystems

Reservoirs of Listeria Species in Three Environmental Ecosystems

The Agr communication system provides a benefit to the populations of Listeria monocytogenes in soil

Survival of Listeria monocytogenes in Soil Requires AgrA-Mediated Regulation

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