NAT screening of blood donors for severe acute respiratory syndrome coronavirus can potentially prevent transfusion associated transmissions

Schmidt, Michael; Brixner, V.; Rüster, Brigitte; Hourfar, M. K.; Drosten, Christian; Preiser, Wolfgang; Seifried, Erhard; Roth, W. Kurt

doi:10.1111/j.1537-2995.2004.03269.x

Cited by 28 publications

(25 citation statements)

References 26 publications

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“…Human SARS‐CoV transmission occurs by close contact with symptomatic infected individuals (Wu et al ., 2004). Thus far, there is no evidence that the virus is transmitted through the blood supply, although a viraemic phase has been described in the first week after the onset of illness (Grant et al ., 2003; Schmidt et al ., 2004). However, the possibility of introduction of the SARS‐CoV into the blood supply should be prevented to avoid reemergence of this infectious agent by human to human route.…”

mentioning

confidence: 99%

Amotosalen photochemical inactivation of severe acute respiratory syndrome coronavirus in human platelet concentrates

Pinna¹,

Sampson‐Johannes

Clementi

et al. 2005

Transfusion Medicine

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A novel human coronavirus causing severe acute respiratory syndrome (SARS) emerged in epidemic form in early 2003 in China and spread worldwide in a few months. Every newly emerging human pathogen is of concern for the safety of the blood supply during and after an epidemic crisis. For this purpose, we have evaluated the inactivation of SARS-coronavirus (CoV) in platelet concentrates using an approved pathogen inactivation device, the INTERCEPT Blood System. Apheresis platelet concentrates (APCs) were inoculated with approximately 10(6) pfu mL(-1) of either Urbani or HSR1 isolates of SARS-CoV. The inoculated units were mixed with 150 microm amotosalen and illuminated with 3 J cm(-2) UV-A light. The viral titres were determined by plaque formation in Vero E6 cells. Mixing SARS-CoV with APC in the absence of any treatment decreased viral infectivity by approximately 0.5-1 log10. Following photochemical treatment, SARS-CoV was consistently inactivated to the limit of detection in seven independent APC units. No infectious virus was detected after treatment when up to one-third of the APC unit was assayed, demonstrating a mean log10-reduction of >6.2. Potent inactivation of SARS-CoV therefore extends the capability of the INTERCEPT Blood System in inactivating a broad spectrum of human pathogens including recently emerging respiratory viruses.

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mentioning

confidence: 99%

Amotosalen photochemical inactivation of severe acute respiratory syndrome coronavirus in human platelet concentrates

Pinna¹,

Sampson‐Johannes

Clementi

et al. 2005

Transfusion Medicine

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“…Real-time PCR testi SARS-CoV viremisini saptayabilir ve salgınlarda transfüzyon güvenliğini arttırmak için kullanılabilir (64,65) .…”

Section: şIddetli Akut Solunum Yetmezliği (Severe Acute Respiratory Sunclassified

Infectious Agents Transmitted by Blood and Blood Component and Impacts of Nükleik Asit Amplifikasyon Test (NAT)

Savasci¹,

Avcı²

2016

IKSST

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ÖZ Kan ve kan¸bileşenleri ile bulaşan enfeksiyonlar, özellikle viral hepatit virüsleri ve insan immün yetmezlik virüsü¸ (HIV), ABSTRACT Especially viral hepatitis viruses and human immunodeficiency virus (HIV) which are transmitted by the transfusion of blood and blood products have been an important public health problem for a long time in the world. Transfusion of blood and blood products is an ideal, easiest and a simplest route for transmission of infectious diseases. It is known that many infectious agents, either bacterial, viral, parasitic and fungal agents may be transmitted by the transfusion of blood and blood products. Nucleic Asit Amplification Test (NAT) provides additional layer of safety to the blood supply because it allows the detection of infectious agents during their incubation periods. Keywords: blood, blood center, infection, nucleic acid amplification test GİRİŞKan ve kan bileşenleri hayati tehlikeye neden olan birçok hastalık ve travmalarda tedavi amaçlı kullanı-lan bileşenlerdir. Kan ve kan bileşenlerinin tek kaynağının insan olması nedeniyle temini çok zor ve maliyet açısından oldukça pahalı bileşenlerdir. Kan naklinin güvenli bir şekilde yapılması, nakil sonrası ortaya çıkabilecek enfeksiyonların önlenebilmesine bağlıdır. Dünyada her yıl milyonlarca ünite kan ve kan bileşenleri kullanılmakta ve alıcıların bir kısmın-da transfüzyona bağlı enfeksiyonlar ortaya çıkmakta-dır. Kan ve kan bileşenlerinin kullanımı aracılığıyla bağışçıda mevcut enfeksiyon etkeninin alıcıya aktarılması tamamıyla engellenememektedir. Bu nedenle amaç, bu tip bulaşma risklerini "kabul edilebilecek kadar düşük" düzeylere indirgemek olmuştur (1) .Kan ve kan bileşenleri ile birçok enfeksiyon etkeni bulaşabilmektedir. Tarama testlerine rağmen neden enfeksiyon bulaşır sorusuna yanıt şu şekilde verilebilir. Transfüzyonla bulaşan enfeksiyon etkenlerinin dolaşımda uzun süre kalmaları, uzun inkübasyon süreleri, mutant suşların varlığı, asemptomatik klinikle seyretmeleri, kan ve kan bileşenlerinde stabilitelerini korumaları önemli özellikleridir. Enfeksiyon etkenleri arasında virüsler en önemli grubu oluştur-maktadır. En fazla sorun olan virüsler; hepatit B

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“…Potential donors are indefinitely deferred who have spent a cumulative 3 months in the United Kingdom between 1980 and 1996, the period when unsafe cattle feeding practices led to an outbreak of bovine spongiform encephalopathy, or "mad cow" disease. Criteria to avoid severe acute respiratory syndrome 66 were in effect at one time, and Gulf War veterans were deferred from blood donation from 1991 to 1993 to avoid the transmission of leishmaniasis. 64 Also indefinitely deferred are those who spent a cumulative 6 months on European military bases or a cumulative 5 years in certain European countries other than the UK, as are those who received a blood transfusion in the UK from 1980 to the present.…”

Section: Donor History Questionnaire and Recipient Safetymentioning

confidence: 99%

Blood Donation and Collection

Zeger¹,

Selogie²,

Shulman³

2007

Blood Banking and Transfusion Medicine

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NAT screening of blood donors for severe acute respiratory syndrome coronavirus can potentially prevent transfusion associated transmissions

Abstract: A real-time CoV PCR test is able to detect SARS-CoV in viremic blood donor samples even in the beginning of the disease when patients present minor clinical symptoms. Thus the assay could potentially help to prevent transfusion-associated SARS-CoV transmissions.

Cited by 28 publications

References 26 publications

Amotosalen photochemical inactivation of severe acute respiratory syndrome coronavirus in human platelet concentrates

Amotosalen photochemical inactivation of severe acute respiratory syndrome coronavirus in human platelet concentrates

Infectious Agents Transmitted by Blood and Blood Component and Impacts of Nükleik Asit Amplifikasyon Test (NAT)

Blood Donation and Collection

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