NaStEP, an essential protein for self-incompatibility in Nicotiana, performs a dual activity as a proteinase inhibitor and as a voltage-dependent channel blocker

Cruz‐Zamora, Yuridia; Nájera-Torres, E.; Noriega‐Navarro, Roxana; Torres-Rodriguez, M.D.; Bernal-Gracida, Lilia; Garcı́a-Valdés, Jesús; Juárez‐Díaz, Javier Andrés; Cruz‐García, Felipe

doi:10.1016/j.plaphy.2020.03.052

Cited by 6 publications

(7 citation statements)

References 41 publications

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“…The stability of HT-B relies on the presence of NaStEP, which protects HT-B from degradation by its proteinase inhibitor activity ( Jiménez-Durán et al., 2013 ). NaStEP also interacts with NaSIPP, a mitochondrial phosphate carrier, to form a complex with a mitochondrial voltage-dependent channel, potentially leading to mitochondrial destabilization and triggering cell death ( García-Valencia et al., 2017 ; Cruz-Zamora et al., 2020 ). Ultimately, the compartmentalization of self S -RNase is disrupted by a yet-unclear specific interaction among these modifier genes.…”

Section: Molecular Basis Of Self-pollen Rejection In Different Si Sys...mentioning

confidence: 99%

Molecular insights into self-incompatibility systems: From evolution to breeding

Zhang,

Li,

Zhao

et al. 2024

Plant Communications

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Section: Molecular Basis Of Self-pollen Rejection In Different Si Sys...mentioning

confidence: 99%

Molecular insights into self-incompatibility systems: From evolution to breeding

Zhang,

Li,

Zhao

et al. 2024

Plant Communications

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“…In compatible crosses, S-specific recognition leads to NaStEP inhibition, allowing a subtilisin-like protease to degrade HT-B (Figure 3B). HT-B loss results in intact vacuoles and S-RNase sequestration (Goldraij et al, 2006;McClure et al, 2011;Cruz-Zamora et al, 2020).…”

Section: Moreels Et Almentioning

confidence: 99%

Intra- and inter-specific reproductive barriers in the tomato clade

Moreels,

Bigot,

Defalque

et al. 2023

Front. Plant Sci.

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Tomato (Solanum lycopersicum L.) domestication and later introduction into Europe resulted in a genetic bottleneck that reduced genetic variation. Crosses with other wild tomato species from the Lycopersicon clade can be used to increase genetic diversity and improve important agronomic traits such as stress tolerance. However, many species in the Lycopersicon clade have intraspecific and interspecific incompatibility, such as gametophytic self-incompatibility and unilateral incompatibility. In this review, we provide an overview of the known incompatibility barriers in Lycopersicon. We begin by addressing the general mechanisms self-incompatibility, as well as more specific mechanisms in the Rosaceae, Papaveraceae, and Solanaceae. Incompatibility in the Lycopersicon clade is discussed, including loss of self-incompatibility, species exhibiting only self-incompatibility and species presenting both self-compatibility and self-incompatibility. We summarize unilateral incompatibility in general and specifically in Lycopersicon, with details on the ’self-compatible x self-incompatible’ rule, implications of self-incompatibility in unilateral incompatibility and self-incompatibility-independent pathways of unilateral incompatibility. Finally, we discuss advances in the understanding of compatibility barriers and their implications for tomato breeding.

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“…The degradation/stabilization of HT-B and 120K indicates that proteolytic activity is essential during SI. Likewise, NaStEP, another pistil-expressed MG, encodes a proteinase inhibitor [39,40]. When NaStEP is suppressed in Nicotiana transgenic plants, the ability to reject self-pollen is abolished.…”

Section: Gsi S-rnase-based Si An Intricated Systemmentioning

confidence: 99%

“…When NaStEP is suppressed in Nicotiana transgenic plants, the ability to reject self-pollen is abolished. In the absence of NaStEP, the HT-B protein is degraded in PT of both compatible and incompatible crosses, suggesting that NaStEP positively regulates HT-B stability in PT [33], inhibiting probably to a putative pollen protease [33,40]. However, direct evidence of this is lacking.…”

Section: Gsi S-rnase-based Si An Intricated Systemmentioning

confidence: 99%

“…Functional assays provide evidence that HT-B is an essential protein in the breakdown of the S-RNase-containing vacuole because, in its absence, the integrity of this vacuole persists [23]. Besides, the stability of HT-B in incompatible PTs depends on the presence of NaStEP, likely inhibiting the protease activity that degrades HT-B in compatible PT through its proteinase inhibitor activity [33,40].…”

Section: Mcda000684 8(2)2021mentioning

confidence: 99%

See 1 more Smart Citation

Self-Incompatibility in S-RNase-Based Systems: Are there Differences in the Pollen Rejection Mechanisms Among Species?

García

Juárez‐Díaz

Zamora

2021

MCDA

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Several barriers occur among flowering plants to prevent self-fertilization by increasing and maintaining the species genetic variability. From all these barriers, self-incompatibility (SI) is one of the most effective avoiding endogamy. Genetic control in SI relies on the polymorphic S locus, where both pollen and pistil S determinants are encoded. Based on the pollen S phenotype, diploid or haploid, there are two kinds of genetic control in the SI systems: the sporophytic and the gametophytic. In the gametophytic SI systems, two mechanisms are currently unraveled. One exclusive to Papaveraceae and the other one, known as the S-RNase-based SI, which is present in more divergent taxa: Plantaginaceae, Rosaceae, Rubiaceae, Rutaceae, and Solanaceae. The more in-depth studies on the molecular mechanism have been mainly done in Solanaceae, particularly in Petunia and Nicotiana, achieving significant advances by different genetic, molecular, biochemical, and cellular approaches, resulting in two models to encompass further evidence to explain it: the collaborative and the compartmentalization model. Both models, however, seem to be excluding each other. Here we will discuss the evidence and the information around these explanations and will discuss the possibility of why it has been so challenging to find an integrative hypothesis to understand this critical evolutionary process within angiosperms.

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NaStEP, an essential protein for self-incompatibility in Nicotiana, performs a dual activity as a proteinase inhibitor and as a voltage-dependent channel blocker

Cited by 6 publications

References 41 publications

Molecular insights into self-incompatibility systems: From evolution to breeding

Molecular insights into self-incompatibility systems: From evolution to breeding

Intra- and inter-specific reproductive barriers in the tomato clade

Self-Incompatibility in S-RNase-Based Systems: Are there Differences in the Pollen Rejection Mechanisms Among Species?

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