Nasal vaccination with poly(β-amino ester)-poly( d , l -lactide- co -glycolide) hybrid nanoparticles

Sinani, Genada; Sessevmez, Melike; Gök, Mehmet Koray; Özgümüş, Saadet; Okyar, Alper; Alpar, H. Oya; Cevher, Erdal

doi:10.1016/j.ijpharm.2017.06.053

Cited by 17 publications

(6 citation statements)

References 84 publications

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“…In mice, AIV encapsulated in a chitosan-poly-(ε-caprolactone) NPs were found to induce higher IgG2a antibodies when administered intranasally, and such responses persisted for a longer time due to a slow-release of antigens from the NPs [ 74 ]. Although we did not assess secretory IgA (sIgA) in the intestinal washes, experiments in mice indicated significant amounts of sIgA production in the intestines and respiratory tracts for intranasally administered polyester coated PLGA-NPs [ 31 ]. The distant mucosal immunity as well as systemic immunity induced following mucosal application of NPs-based vaccines are believed to be due to trafficking of APCs phagocytizing NPs for presenting antigens to B and T cells residing in other lymphoid tissues [ 75 ].…”

Section: Discussionmentioning

confidence: 99%

“…Delivering PLGA NPs-based vaccines through the oral and nasal routes improved the immunogenicity of several recombinant and conventional vaccines derived from human and veterinary pathogens [ 27 – 29 ]. PLGA NPs are flexible and tunable in that their outer surface can be modified with other polymers such as chitosan or poly(β-amino esters) for more effective mucosal vaccine delivery [ 30 , 31 ]. The mucoadhesive property of chitosan and its derivative N-trimethyl chitosan allows better interactions of nanoparticles with mucus, which then improves the residence time of vaccines on mucosal surfaces and, thus, over time facilitates cellular uptake of antigens [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of immunogenicity of avian influenza antigens encapsulated in PLGA nanoparticles following mucosal and subcutaneous delivery in chickens

et al. 2018

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Mucosal vaccine delivery systems have paramount importance for the induction of mucosal antibody responses. Two studies were conducted to evaluate immunogenicity of inactivated AIV antigens encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles (NPs). In the first study, seven groups of specific pathogen free (SPF) layer-type chickens were immunized subcutaneously at 7-days of age with different vaccine formulations followed by booster vaccinations two weeks later. Immune responses were profiled by measuring antibody (Ab) responses in sera and lachrymal secretions of vaccinated chickens. The results indicated that inactivated AIV and CpG ODN co-encapsulated in PLGA NPs (2x NanoAI+CpG) produced higher amounts of hemagglutination inhibiting antibodies compared to a group vaccinated with non-adjuvanted AIV encapsulated in PLGA NPs (NanoAI). The tested adjuvanted NPs-based vaccine (2x NanoAI+CpG) resulted in higher IgG responses in the sera and lachrymal secretions at weeks 3, 4 and 5 post-vaccination when immunized subcutaneously. The incorporation of CpG ODN led to an increase in Ab-mediated responses and was found useful to be included both in the prime and booster vaccinations. In the second study, the ability of chitosan and mannan coated PLGA NPs that encapsulated AIV and CpG ODN was evaluated for inducing antibody responses when delivered via nasal and ocular routes in one-week-old SPF layer-type chickens. These PLGA NPs-based and surface modified formulations induced robust AIV-specific antibody responses in sera and lachrymal secretions. Chitosan coated PLGA NPs resulted in the production of large quantities of lachrymal IgA and IgG compared to mannan coated NPs, which also induced detectable amounts of IgA in addition to the induction of IgG in lachrymal secretions. In both mucosal and subcutaneous vaccination approaches, although NPs delivery enhanced Ab-mediated immunity, one booster vaccination was required to generate significant amount of Abs. These results highlight the potential of NPs-based AIV antigens for promoting the induction of both systemic and mucosal immune responses against respiratory pathogens.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of immunogenicity of avian influenza antigens encapsulated in PLGA nanoparticles following mucosal and subcutaneous delivery in chickens

et al. 2018

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“…Due to their unique features, including enhanced Ag immunogenicity, increased Ag adsorption and uptake by antigen presenting cells (APCs) and Ag protection from in vivo environment, nanovaccines are expected to address the limitations associated with the use of conventional live and inactivated vaccines such as return to virulence, incomplete inactivation and sometimes, weak immune responses . Numerous synthetic and semisynthetic nanoparticles based on organic, inorganic, and hydrid materials have been reported as potential nanovaccines . Among them, self‐assembling β‐sheet peptides, or amyloid‐like, were recently being investigated as potential nanoplatform for vaccination due to their biocompatibility and biodegradability.…”

Section: Self‐assembled Nanovaccinesmentioning

confidence: 99%

Amyloid self‐assembling peptides: Potential applications in nanovaccine engineering and biosensing

et al. 2018

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Living organisms are an inestimable source of inspiration for the design of biomaterials and nanostructures for medical and technological applications. Amyloids, which were historically associated with diseases, have recently been recognized as a biological structure that performs vital physiological functions in host organisms, highlighting their potential as life‐inspired assemblies. Amyloids are highly organized proteinaceous assemblies characterized by a cross‐β‐sheet quaternary conformation. The mechanical, physical, and biological properties of amyloids suggest that these nanostructures hold great potential as soft materials, nanoparticles, and biomatrices. This potential is associated with many characteristics, including the spontaneous self‐assembly of many polypeptide sequences, high mechanical resistance, biocompatibility, biodegradability as well as thermal, chemical, and enzymatic stability. Moreover, peptide‐based amyloid assemblies can efficiently be obtained by standard solid phase peptide synthesis and orthogonally functionalized with a wide range of biomolecules, such as large proteins and DNA. In this review, after briefly introducing the amyloid structure and the mechanisms of self‐assembly, we describe approaches to identify and design short self‐assembling amyloidogenic peptides. Afterward, we introduce strategies used to functionalize amyloid materials and we highlight some relevant examples in the development of nanovaccines and biosensors.

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“…Hydrogels are selected by researchers in pharmaceutical and medicine as biomaterials due to their ability to respond to changes in their environment like as temperature, pH, heat, light, magnetic and electrical field, etc. These properties of hydrogel allow them to utilize releasing of active substances [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Photopolymerizable HEMA/PEG-DA Based Hydrogels Filled with Low Concentrations of Nanoparticle Titanium Dioxide for Release Of Donepezil HCl

Şenol¹,

Akyol²

2021

El-Cezeri Fen Ve Mühendislik Dergisi

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2-hydroxyl ethyl methacrylate/poly(ethylene glycol) diacrylate (HEMA/PEG-DA) based hydrogels are attractive drug carriers due to their appealing properties such as biodegradability and sustained release. The scope of this study was to investigate the swelling and release behaviors of HEMA/PEG-DA-based hydrogels filled with low concentrations of nanoparticle titanium dioxide (TiO 2 ). Hydrogels have been synthesized successfully by photopolymerization. 2,2-dimethoxy-2-phenyl-acetophenone (Irgacure 651), 1hydroxycyclohexyl phenyl ketone (Irgacure 184) and 2-hydroxy-4'-(2-hydroxyethoxy)-2 methylpropiophenone (Irgacure 2959) were selected for photopolymerization. Fourier Transform Infrared Spectroscopy (FT-IR) had been used in confirming the functional group of hydrogels. A digital microscope and Scanning Electron Microscope (SEM) were used for characterizing synthesized hydrogels. This study revealed that the content of hydrogels, the kind of photo-initiators and pH have a significant effect on the swelling and releasing of Donepezil Hydrochloride (active pharmaceutical ingredient (API) for Alzheimer disease) behaviours.

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Nasal vaccination with poly(β-amino ester)-poly( d , l -lactide- co -glycolide) hybrid nanoparticles

Cited by 17 publications

References 84 publications

Characterization of immunogenicity of avian influenza antigens encapsulated in PLGA nanoparticles following mucosal and subcutaneous delivery in chickens

Characterization of immunogenicity of avian influenza antigens encapsulated in PLGA nanoparticles following mucosal and subcutaneous delivery in chickens

Amyloid self‐assembling peptides: Potential applications in nanovaccine engineering and biosensing

Preparation of Photopolymerizable HEMA/PEG-DA Based Hydrogels Filled with Low Concentrations of Nanoparticle Titanium Dioxide for Release Of Donepezil HCl

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