Nasal microbiota dominated by Moraxella spp. is associated with respiratory health in the elderly population: a case control study

Munckhof, Ellen H A van den; Hafkamp, Harriet C.; Kluijver, Josephine de; Kuijper, Ed J.; Koning, Maurits N.C. de; Quint, Wim; Knetsch, Cornelis W.

doi:10.1186/s12931-020-01443-8

Cited by 16 publications

(9 citation statements)

References 27 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The genus Moraxella and its family, largely represented in our samples, have been found in high proportions in samples of donors, and in samples with low levels of global DNA fragmentation and better sperm morphology. Beneficial effects of Moraxella have also been observed in both respiratory and intestinal tracts, where stable colonies of this genus have been associated with good health and its deregulation has been linked to pathogenic effects ( Clemente et al, 2012 ; Biesbroek et al, 2014 ; Bosch et al, 2017 ; van den Munckhof et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Seminal Microbiota of Idiopathic Infertile Patients and Its Relationship With Sperm DNA Integrity

Garcia-Segura

Rey

Closa

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

The development of new biomarkers for human male infertility is crucial to improve the diagnosis and the prognosis of this disease. Recently, seminal microbiota was shown to be related to sperm quality parameters, suggesting an effect in human fertility and postulating it as a biomarker candidate. However, its relationship to sperm DNA integrity has not been studied yet. The aim of the present study is to characterize the seminal microbiota of a western Mediterranean population and to evaluate its relationship to sperm chromatin integrity parameters, and oxidative stress. For that purpose, 14 samples from sperm donors and 42 samples from infertile idiopathic patients were obtained and were analyzed to assess the composition of the microbiota through full-length 16S rRNA gene sequencing (Illumina MiSeq platform). Microbial diversity and relative abundances were compared to classic sperm quality parameters (macroscopic semen parameters, motility, morphology and concentration), chromatin integrity (global DNA damage, double-stranded DNA breaks and DNA protamination status) and oxidative stress levels (oxidation-reduction potential). The seminal microbiota observed of these samples belonged to the phyla Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes. The most abundant genera were Finegoldia, Peptoniphilus, Anaerococcus, Campylobacter, Streptococcus, Staphylococcus, Moraxella, Prevotella, Ezakiella, Corynebacterium and Lactobacillus. To our knowledge, this is the first detection of Ezakiella genus in seminal samples. Two clusters of microbial profiles were built based on a clustering analysis, and specific genera were found with different frequencies in relation to seminal quality defects. The abundances of several bacteria negatively correlate with the sperm global DNA fragmentation, most notably Moraxella, Brevundimonas and Flavobacterium. The latter two were also associated with higher sperm motility and Brevundimonas additionally with lower oxidative-reduction potential. Actinomycetaceae, Ralstonia and Paenibacillus correlated with reduced chromatin protamination status and increased double-stranded DNA fragmentation. These effects on DNA integrity coincide in many cases with the metabolism or enzymatic activities of these genera. Significant differences between fertile and infertile men were found in the relative presence of the Propionibacteriaceae family and the Cutibacterium, Rhodopseudomonas and Oligotropha genera, which supports its possible involvement in male fertility. Our findings sustain the hypothesis that the seminal microbiome has an effect on male fertility.

show abstract

Section: Discussionmentioning

confidence: 99%

Seminal Microbiota of Idiopathic Infertile Patients and Its Relationship With Sperm DNA Integrity

Garcia-Segura

Rey

Closa

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…are a part of the normal flora of the human respiratory and genital tracts and are considered to have low pathogenic potential. Among Moraxella spp., the most clinically relevant microorganism from this genus is M. catarrhalis , which can cause otitis media in children and exacerbations of chronic obstructive pulmonary disease [ 7 , 8 ]. Other members of the Moraxella genus, such as M. lacunata , M. osloensis , and M. nonliquefaciens rarely cause disease.…”

Section: Discussionmentioning

confidence: 99%

Late-Onset Bleb-Related Endophthalmitis Caused by Moraxella nonliquefaciens: A Case Report

Shen

Chen

2023

Antibiotics

View full text Add to dashboard Cite

Moraxella species are Gram-negative coccobacilli that typically colonize the flora of the human upper respiratory tract and have low pathogenic potential. There are limited case reports implicating the organisms as the cause of endocarditis, bacteremia, septic arthritis, ocular infection, and meningitis. In cases of keratitis and conjunctivitis, Moraxella nonliquefaciens is not commonly isolated from the ocular surface. We present a case of a diabetic patient who developed late-onset bleb-related endophthalmitis caused by M. nonliquefaciens 4 years after glaucoma filtering surgery. Within one day, the patient presented with an acutely fulminant course with sudden visual loss, redness, and ocular pain. Appropriate antibiotic treatment and early vitrectomy resulted in a favorable final visual acuity of 20/100, which was his vision prior to infection. The use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass spectrometry (MALDI-TOF MS) enabled the rapid identification of the organism. Endophthalmitis caused by M. nonliquefaciens should be considered in patients who underwent glaucoma filtering surgery with antifibrotic agents.

show abstract

“…might be associated with a healthier status in the elderly at risk for RTIs, while in the pediatric populations, the reports on this health–associated taxa ( Man et al., 2020 ) are more conflicting as far as microbiota profile stability and association with RTIs ( Bosch and McFall–Ngai, 2011 ; Biesbroek et al., 2014 ; Teo et al., 2015 ; Man et al., 2019 ). The differences in susceptibility to RTIs likely arise from the complex interplay between mucosa, innate and adaptive immunity, and airway microbiota ( van den Munckhof et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Microbiota profiles in pre-school children with respiratory infections: Modifications induced by the oral bacterial lysate OM-85

Esposito

Ballarini

Argentiero

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

To describe microbiota profiles considering potential influencing factors in pre-school children with recurrent respiratory tract infections (rRTIs) and to evaluate microbiota changes associated with oral bacterial lysate OM-85 treatment, we analyzed gut and nasopharynx (NP) microbiota composition in patients included in the OM-85-pediatric rRTIs (OMPeR) clinical trial (https://www.clinicaltrialsregister.eu/ctr-search/trial/2016-002705-19/IT). Relative percentage abundance was used to describe microbiota profiles in all the available biological specimens, grouped by age, atopy, and rRTIs both at inclusion (T0) and at the end of the study, after treatment with OM-85 or placebo (T1). At T0, Firmicutes and Bacteriodetes were the predominant genera in gut and Proteobacteria, Firmicutes, and Actinobacteria were the predominant genera in NP samples. Gut microbiota relative composition differed with age (<2 vs. ≥2 years) for Firmicutes, Proteobacteria, Actinobacteria (phyla) and Bifidobacterium, Ruminococcus, Lachnospiraceae (genera) (p < 0.05). Moraxella was more enriched in the NP of patients with a history of up to three RTIs. Intra-group changes in relative percentage abundance were described only for patients with gut and NP microbiota analysis available at both T0 and T1 for each study arm. In this preliminary analysis, the gut microbiota seemed more stable over the 6-month study in the OM-85 group, whose mean age was lower, as compared to the placebo group (p = 0.004). In this latter group, the relative abundance of Bacteroides decreased significantly in children ≥2 years. Some longitudinal significant differences in genera relative abundance were also detected in children of ≥2 years for NP Actinobacteria, Haemophilus, and Corynebacterium in the placebo group only. Due to the small number of patients in the different sub-populations, we could not identify significant differences in the clinical outcome and therefore no associations with microbiota changes were searched. The use of bacterial lysates might play a role in microbiota rearrangement, but further data and advanced analysis are needed to prove this in less heterogeneous populations with higher numbers of samples considering the multiple influencing factors such as delivery method, age, environment, diet, antibiotic use, and type of infections to ultimately show any associations with prevention of rRTIs.

show abstract

Nasal microbiota dominated by Moraxella spp. is associated with respiratory health in the elderly population: a case control study

Cited by 16 publications

References 27 publications

Seminal Microbiota of Idiopathic Infertile Patients and Its Relationship With Sperm DNA Integrity

Seminal Microbiota of Idiopathic Infertile Patients and Its Relationship With Sperm DNA Integrity

Late-Onset Bleb-Related Endophthalmitis Caused by Moraxella nonliquefaciens: A Case Report

Microbiota profiles in pre-school children with respiratory infections: Modifications induced by the oral bacterial lysate OM-85

Contact Info

Product

Resources

About