Nasal lavage as a tool for the assessment of upper-airway inflammation in adults and children

Nikasinovic-Fournier, Lydia; Just, Jocelyne; Seta, Nathalie; Callais, F.; Sahraoui, F.; Grimfeld, A; Momas, Isabelle

doi:10.1067/mlc.2002.121661

Cited by 22 publications

(25 citation statements)

References 29 publications

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“…Previous articles reported nasal lavage cell distribution in healthy controls (Nikasinovic-Fournier et al 2002;Prat et al 1993;Kinhult et al 2003), but to the best of our knowledge neither has the speciWc cell distribution previously been described in such a large number of subjects, nor has a cut-oV to objectively deWne nasal eosinophilic inXammation been proposed.…”

Section: Discussionmentioning

confidence: 84%

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Nasal blown secretion evaluation in specific occupational nasal challenges

Pignatti

Pala

Pisati

et al. 2009

Int Arch Occup Environ Health

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Section: Discussionmentioning

confidence: 84%

“…Another concern may regard the reproducibility of nasal eosinophils (Howarth et al 2005;Castano et al 2008;Nikasinovic-Fournier et al 2002). In our study the eosinophil intra-subject reproducibility, evaluated in AR patients in whom a minimal persistent inXammation exists (Bousquet et al 2008), was good.…”

Section: Discussionmentioning

confidence: 90%

Nasal blown secretion evaluation in specific occupational nasal challenges

Pignatti

Pala

Pisati

et al. 2009

Int Arch Occup Environ Health

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“…Nasal inflammation was assessed by using a noninvasive, well-tolerated, and reproducible method. 24 All nasal biomarkers, apart from eosinophils, did not differ between asthmatic and healthy children. One possible explanation is that all children living in urban areas could present with increased NALF mediators, whatever their health status.…”

Section: Methodological Issuesmentioning

confidence: 98%

“…Nasal response was evaluated by using an NAL method adapted from the Hilding procedure for children. 24 NAL consisted of irrigation of the nasal cavity (3 instillation/aspiration cycles for each nostril) with saline solution (NaCl 9%) through a transparent inflated pediatric Foley catheter (Porgès SA, Le Plessis Robinson, France). The NALFs recovered from each nostril were pooled and centrifuged (500g) for 15 minutes at 14°C.…”

Section: Methodsmentioning

confidence: 99%

Nasal inflammation and personal exposure to fine particles PM2.5 in asthmatic children

Nikasinovic¹,

Just

Sahraoui

et al. 2006

Journal of Allergy and Clinical Immunology

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“…There are different parameters that can influence the results of the nasal lavage. Studies have shown that time of day, duration of solution in the vestibule, temperature of the solution, performing pre-washing or not, number of repeated measures each day and days in a row may all influence the results gained [173,[180][181]. The concentration, volume and presence of various markers may be altered due to these factors and consequently they are significant to consider when performing a study.…”

Section: Nasal Lavagementioning

confidence: 99%

Upper Airway Mucosal Inflammation : Proteomic Studies after Exposure to Irritants and Microbial Agents

Fornander¹

2015

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People are, in their daily lives, exposed to a number of airborne foreign compounds that do not normally affect the body. However, depending on the nature of these compounds, dose and duration of exposure, various airway symptoms may arise. Early symptoms are often manifested as upper airway mucosal inflammation which generates changes in protein composition in the airway lining fluid.This thesis aims at identifying, understanding mechanisms and characterizing protein alterations in the upper airway mucosa that can be used as potential new biomarkers for inflammation in the mucosa. The protein composition in the mucosa was studied by sampling of nasal lavage fluid that was further analyzed with a proteomic approach using two-dimensional gel electrophoresis and mass spectrometry. Additionally, by studying factors on site through environmental examination, health questionnaires and biological analyses, we have tried to understand the background to these protein alterations and their impact on health.Respiratory symptoms from the upper airways are common among people who are exposed to irritative and microbial agents. This thesis have focused on personnel in swimming pool facilities exposed to trichloramine, metal industry workers exposed to metalworking fluids, employees working in damp and moldy buildings and infants diagnosed with respiratory syncytial virus infection. The common denominator in these four studies is that the subjects experience upper airway mucosal inflammation, which is manifested as cough, rhinitis, phlegm etc. In the three occupational studies, the symptoms were work related. Notably, a high prevalence of perceived mucosal symptoms was shown despite the relatively low levels of airborne irritants revealed by the environmental examination. Protein profiling verified an ongoing inflammatory response by identification of several proteins that displayed altered levels. Interestingly, innate immune proteins dominated and four protein alterations occurred in most of the studies; SPLUNC1, protein S100A8 and S100A9 and alpha-1-antitrypsin. Similarly, these proteins were also found in nasal fluid from children with virus infection and in addition a truncated form of SPLUNC1 and two other S100 proteins (S100A7-like 2 and S100A16), not previously found in nasal secretion, were identified.Altogether, the results indicate the potential use of a proteomic approach for identifying new biomarkers for the upper respiratory tract at an early stage in the disease process after exposure to irritant and microbial agents. The results indicate an effect on the innate immunity system and the proteins; SPLUNC1, protein S100A8 and S100A9 and alpha-1-antitrypsin are especially promising new biomarkers. Moreover, further studies of these proteins may help us to understand the molecular mechanisms involved in irritant-induced airway inflammation. POPULÄRVETENSKAPLIG SAMMANFATTNING FÖRÄNDRAD PROTEINSAMMANSÄTTNING I DE ÖVRE LUFTVÄGARNA EFTER EXPONERING FÖR SLEMHINNEIRRITERANDE ÄMNENVarje dag utsätts vi för partikla...

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Nasal lavage as a tool for the assessment of upper-airway inflammation in adults and children

Cited by 22 publications

References 29 publications

Nasal blown secretion evaluation in specific occupational nasal challenges

Nasal blown secretion evaluation in specific occupational nasal challenges

Nasal inflammation and personal exposure to fine particles PM2.5 in asthmatic children

Upper Airway Mucosal Inflammation : Proteomic Studies after Exposure to Irritants and Microbial Agents

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