Nasal immunization studies using liposomes loaded with tetanus toxoid and CpG-ODN

Tafaghodi, Mohsen; Jaafari, M. R.; Tabassi, Sayyed Abolghasem Sajadi

doi:10.1016/j.ejpb.2006.05.005

Cited by 52 publications

(10 citation statements)

References 27 publications

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“…Alpar et al106 reported a significantly higher serum immune response in Dunkin Hartley guinea pigs with a liposome formulation compared to the same quantity of free protein in solution form ( p < 0.05). However, in a more recent study Tafaghodi et al107 reported the highest serum IgG and antitoxin titres in groups of white albino rabbits that were intranasally immunized with tetanus toxoid solution formulation ( p < 0.001). In contrast the mucosal immune response (sIgA titres in lungs and nasal cavity) was highest with the liposome formulation.…”

Section: Particulate Systems For Intranasal Vaccine Deliverymentioning

confidence: 94%

Pharmaceutical Aspects of Intranasal Delivery of Vaccines Using Particulate Systems

Sharma

Mukkur

Benson

et al. 2009

Journal of Pharmaceutical Sciences

145

137

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Section: Particulate Systems For Intranasal Vaccine Deliverymentioning

confidence: 94%

Pharmaceutical Aspects of Intranasal Delivery of Vaccines Using Particulate Systems

Sharma

Mukkur

Benson

et al. 2009

Journal of Pharmaceutical Sciences

145

137

View full text Add to dashboard Cite

“…Sodium alginate, a natural polysaccharide polymer, can be easily cross-linked into a solid matrix with the addition of di- or trivalent cations (cross-linking in a water-in-oil emulsion results in the formation of microspheres). Alginate microspheres were used in several studies and showed good potential as vaccine delivery system and immunoadjuvant (8-10). Immunization of animals by alginate microspheres containing antigenic proteins elicited both humoral and cell-mediated immune responses (11).…”

Section: Introductionmentioning

confidence: 99%

Immunization against leishmaniasis by PLGA nanospheres encapsulated with autoclaved Leishmania major (ALM) and CpG-ODN

2010

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Various adjuvants and delivery systems have been evaluated for increasing the protective immune responses against leishmaniasis and mostly have been shown not to be effective enough. In this study, poly(D,L-lactide-co-glycolide) (PLGA) nanospheres as an antigen delivery system and CpG-ODN as an immunoadjuvant have been used for the first time to enhance the immune response against autoclaved Leishmania major (ALM). PLGA nanospheres were prepared by a double-emulsion (W/O/W) technique. Particulate characteristics were studied by scanning electron microscopy and particle size analysis. Mean diameter of ALM + CpG-ODN-loaded nanospheres was 300 ± 128 nm. BALB/c mice were immunized three times in 3-week intervals using ALM plus CpG-ODN-loaded nanospheres [(ALM + CpG-ODN)(PLGA)], ALM encapsulated PLGA nanospheres [(ALM)(PLGA)], (ALM)(PLGA) + CpG, ALM + CpG, ALM alone, or phosphate buffer solution (PBS). The intensity of infection induced by L. major challenge was assessed by measuring size of footpad swelling. The strongest protection, showed by significantly (P<0.05) smaller footpad, was observed in mice immunized with (ALM + CpG-ODN)(PLGA). The (ALM)(PLGA), (ALM)(PLGA) + CpG, and ALM + CpG were also showed a significantly (P<0.05) smaller footpad swelling compared to the groups received either PBS or ALM alone. The mice immunized with (ALM + CpG-ODN)(PLGA), (ALM)(PLGA) + CpG, and ALM + CpG showed the highest IgG2a/IgG1 ratio, interferon-γ production, and lowest interleukin-4 production compared to the other groups. It is concluded that when both PLGA nanospheres and CpG-ODN adjuvants were used simultaneously, it induce stronger immune response and enhance protection rate against Leishmania infection.

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“…Similarly, treatment by IND over a hundred-fold dose range did not cause any toxicity. Histological examination of the nasal mucosa was not performed as prior studies did not observe any congestion, edema, epithelial sloughing, necrosis or hemorrhage of the nasal mucosa with administration of plain liposomes in human volunteers [29] or calcitonin loaded liposomes in rats [30]. In the CED, reflux of infused nal-IRI to the upper parenchymal brain was observed (Figure 2A).…”

Section: Discussionmentioning

confidence: 96%

New therapeutic approaches for brainstem tumors: a comparison of delivery routes using nanoliposomal irinotecan in an animal model

Louis

Liu

et al. 2017

J Neurooncol

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Despite the advances in imaging, surgery and radiotherapy, the majority of patients with brainstem gliomas die within 2 years after initial diagnosis. Factors that contribute to the dismal prognosis of these patients include the infiltrative nature and anatomic location in an eloquent area of the brain, which prevents total surgical resection and the presence of the blood-brain barrier (BBB), which reduces the distribution of systemically administered agents. The development of new therapeutic approaches which can circumvent the BBB is a potential path to improve outcomes for these children. Convection-enhanced delivery (CED) and intranasal delivery (IND) are strategies that permit direct drug delivery into the central nervous system and are an alternative to intravenous injection (IV). We treated rats bearing human brainstem tumor xenografts with nanoliposomal irinotecan (CPT-11) using CED, IND, and IV. A single treatment of CED irinotecan had a similar effect on overall survival as multiple treatments by IV route. IND CPT-11 showed significantly increased survival of animals with brainstem tumors, and demonstrated the promise of this non-invasive approach of drug delivery bypassing the BBB when combined with nanoliposomal chemotherapy. Our results indicated that using CED and IND of nanoliposomal therapy increase likelihood of practical therapeutic approach for the treatment of brainstem gliomas.

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Nasal immunization studies using liposomes loaded with tetanus toxoid and CpG-ODN

Cited by 52 publications

References 27 publications

Pharmaceutical Aspects of Intranasal Delivery of Vaccines Using Particulate Systems

Pharmaceutical Aspects of Intranasal Delivery of Vaccines Using Particulate Systems

Immunization against leishmaniasis by PLGA nanospheres encapsulated with autoclaved Leishmania major (ALM) and CpG-ODN

New therapeutic approaches for brainstem tumors: a comparison of delivery routes using nanoliposomal irinotecan in an animal model

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