Nasal Carriage and Resistance Pattern of Multidrug Resistant Staphylococcus aureus Among Healthy Children in Kashan, Iran

Erami, Mahzad; Soltani, Behzad; Ardakani, Abbas Taghavi; Moravveji, Alireza; Rezaei, Mostafa Haji; Soltani, Siamak; Moniri, Rezvan

doi:10.5812/ircmj.21346

Cited by 23 publications

(21 citation statements)

References 28 publications

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“…This frequency is lower than those obtained in the previous studies. 3,[13][14][15] Meanwhile, our findings revealed that 46.7% of the isolates were MRSA, which is in agreement with the study of Dadashi et al (43.0%). 16 It has been noted that the prevalence of MRSA has increased from the year 2000, which partly explains the high prevalence of MRSA observed in our study.…”

Section: Discussionsupporting

confidence: 91%

Nasal Carriage and Antibiotic Resistance Pattern of Methicillin-Resistant Staphylococcus aureus Isolates from Clinical Staff of a Referral Hospital, Zabol, Iran

Mir

Kordi

Ghalehnoo

et al. 2019

Int J Basic Sci Med

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Introduction: Staphylococcus aureus is known as the causative agent of various infections in humans, and the nasal cavity is the main anatomical site for storing and spreading this pathogen in health care workers (HCWs) in hospital settings. Methicillin-resistant S. aureus (MRSA) strains are resistant to many antibiotics. The aim of this study was to investigate the prevalence and antibiotic resistance patterns of S. aureus strains isolated from the nasal carriage of HCWs in a referral hospital in Zabol. Methods: A total of 277 HCWs of Amir-Al-Momenin hospital of Zabol participated voluntarily in this study from March to September 2017. Demographic information was collected using questionnaire. Bacterial isolates were collected from anterior nasal canal using cotton swabs. MRSA isolates were detected by Oxacillin Screen agar and polymerase chain reaction (PCR). Antibiotic resistance was assessed by the disk-diffusion method.Results: In total, 10.8% of HCWs were carriers of S. aureus and 46.7% of the isolates were found to be MRSA. Laboratory staffs were the most frequently colonized HCWs. All the isolates were susceptible to vancomycin, teicoplanin and gentamicin. The highest resistance rate (64.3%) of MRSA isolates was observed against erythromycin.Conclusion: Our findings showed that gentamycin and rifampin might be useful to eradicate S.aureus. Regular screening of HCWs and assessment of antibiotic resistance profile are essential to prevent MRSA dissemination in hospitals.

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Section: Discussionsupporting

confidence: 91%

Nasal Carriage and Antibiotic Resistance Pattern of Methicillin-Resistant Staphylococcus aureus Isolates from Clinical Staff of a Referral Hospital, Zabol, Iran

Mir

Kordi

Ghalehnoo

et al. 2019

Int J Basic Sci Med

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“…In the present study, from 60 S. aureus-positive isolates, 31.66% (19 cases) were MDR-positive. This result is consistent with a previous study in Kashan, Iran, that reported 26.3% of healthy children were nasal carriers of S. aureus, 29.3% were MDR-positive, and 35.9% were MRSA-positive (23).…”

Section: Discussionsupporting

confidence: 93%

Incidence of Oxacillin-Susceptible mecA-Positive Staphylococcus aureus (OS-MRSA) Isolates and TSST-1 Virulence Factor Among High School Students in Tabriz, Northwest of Iran

Ahrabi

Rahbarnia

Dehnad

et al. 2019

Arch Clin Infect Dis

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Background: Methicillin-resistant Staphylococcus aureus (MRSA) is often the cause of a wide broad of infections ranging from minor skin infections to serious infections such as toxic shock syndrome (TSS) in hospital and community settings. Toxic shock syndrome toxin 1 (TSST-1) superantigen is the main cause of TSS. Objectives: The current study aimed to determine the frequency of MRSA carriage and TSST-1 virulence factor among high school students. Methods: A total of 400 nasal swab samples were randomly collected from male and female students of six different high schools in Tabriz, Iran. After confirmation of S. aureus strains by standard biochemical tests, the antibiotic sensitivity pattern of the isolates was determined by the disk diffusion method. The presence of mecA and TSST-1 genes was examined by PCR. Results: From 400 students, 15% (n = 60) were positive for S. aureus. Antimicrobial susceptibility testing was performed using 12 antibiotic disks. Based on the results, 100% of the isolates were resistant to ampicillin while all of them were sensitive to vancomycin. Moreover, 18.34% of the S. aureus isolates were resistant to cefoxitin and 11.67% were resistant to oxacillin. The presence of the mecA gene was confirmed in 54.54% of the cases. These results indicated oxacillin-susceptible mecA-positive S. aureus (OS-MRSA) colonization among students. Based on the PCR results, only one of the MRSA isolates belonging to a female student was positive for the TSST-1 gene. Conclusions: Our findings highlight the spread of OS-MRSA among the healthy population in Tabriz, Northwest of Iran. Nevertheless, a combination of genetic and phenotypic tests is needed to accurately detect MRSA.

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“…In a similar survey done in 2019, the outbreak of MRSA in males was more than female [23]. In the present study, 31.66% of the isolates were positive MDR, similar to a research done in Kashan, IRAN, with a prevalence of 29.3% MDR [24].…”

Section: Discussionsupporting

confidence: 90%

The Prevalence of ACME-arcA and PVL Genes Among Staphylococcus Aureus Isolates in a Student Population from North-West of Iran

Dehnad¹,

Rad²,

Rahbarnia³

et al. 2020

Preprint

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Objectives: Staphylococcus aureus (S. aureus) is the most prevalent cause of skin infections, especially in colonized individuals. Panton–Valentine leukocidin (PVL) and Arginine catabolic mobile element (ACME) are known as the most common virulence factors of S. aureus. This cross-sectional study was conducted to examine the prevalence of ACME-arcA and PVL genes among S.aureus isolates in the student population. Nasal swab samples were randomly collected from 400 healthy students from Tabriz, Iran. The antibiotic resistance pattern of S.aureus isolates was examined by the disk diffusion method. The presence of ACME-arcA, PVL, and mecA genes was detected by PCR reaction.Results: overall, 15% (60/400) students were nasal carriage of S. aureus and 2.75 % (11/400) were MRSA carriage. The frequency of mecA, ACME-arcA, and PVL genes was 54.54% (36/60), 46.66% (28/60), and 16.66% (10/60) respectively. The prevalence of ACME-arcA and PVL genes was independent of gender (P =0.142, P=0.337, respectively). A notable association was observed between the existence of ACME-arcA gene and the frequency of mecA gene (P <0.05), while the incidence of PVL was independent on mecA. These findings highlight the necessity of monitoring nasal carriers in a healthy community to prevent subsequent infections.

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Nasal Carriage and Resistance Pattern of Multidrug Resistant Staphylococcus aureus Among Healthy Children in Kashan, Iran

Cited by 23 publications

References 28 publications

Nasal Carriage and Antibiotic Resistance Pattern of Methicillin-Resistant Staphylococcus aureus Isolates from Clinical Staff of a Referral Hospital, Zabol, Iran

Nasal Carriage and Antibiotic Resistance Pattern of Methicillin-Resistant Staphylococcus aureus Isolates from Clinical Staff of a Referral Hospital, Zabol, Iran

Incidence of Oxacillin-Susceptible mecA-Positive Staphylococcus aureus (OS-MRSA) Isolates and TSST-1 Virulence Factor Among High School Students in Tabriz, Northwest of Iran

The Prevalence of ACME-arcA and PVL Genes Among Staphylococcus Aureus Isolates in a Student Population from North-West of Iran

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