Narrow-band UVB induces apoptosis in human keratinocytes

Aufiero, Barbara; Talwar, H. S.; Young, Chen; Krishnan, Murali; Hatfield, James S.; Lee, Hae Kyung; Wong, Henry K.; Hamzavi, Iltefat; Murakawa, George J.

doi:10.1016/j.jphotobiol.2005.08.011

Cited by 60 publications

(56 citation statements)

References 30 publications

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“…3,10 Given their strong immunomodulatory effects, 36,109 these data are consistent with the cytokinedriven model for AD, although this remains unproved because both treatments have direct effects on keratinocytes. 35,110 However, these studies established the AD phenotype as reversible by using objective biomarkers (Fig 2, B).…”

Section: Evolving Pathogenic Concepts In Admentioning

confidence: 99%

The translational revolution and use of biologics in patients with inflammatory skin diseases

Noda

Krueger

Guttman‐Yassky

2015

Journal of Allergy and Clinical Immunology

177

178

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Section: Evolving Pathogenic Concepts In Admentioning

confidence: 99%

The translational revolution and use of biologics in patients with inflammatory skin diseases

Noda

Krueger

Guttman‐Yassky

2015

Journal of Allergy and Clinical Immunology

177

178

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“…As shown by Schindl et al [19], using human squamous cell carcinoma-derived cell line a dose of 2048 mJ/cm 2 of NB-UVB was required to obtain 50%–60% of cells positive for propidium iodide, which identifies cells with the loss of membrane integrity that is characteristic feature of later phases of cell death. However, a significant degree of apoptosis was already observed at the doses of about 800–1000 mJ/cm 2 depending on studied cell types and conditions of irradiation [20,22]. Importantly, an apoptotic dose of NB-UVB is very similar to the minimal erythema dose (MED) of NB-UVB observed by Gloor and Scherotzke [32] in Caucasian subjects, indicating that indeed UV-induced erythema might be an inflammatory response to the appearance of “sunburn cells” ( i.e ., apoptotic cells) in native human epidermis.…”

Section: Narrow Band Uvb (311 Nm) and Apoptosis Of Keratinocytesmentioning

confidence: 99%

Effects of Narrow Band UVB (311 nm) Irradiation on Epidermal Cells

Reich

Mędrek

2013

IJMS

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Ultraviolet radiation (UVR) is known to be one of the most important environmental hazards acting on the skin. It was revealed that chronic exposure to UVR accelerates skin aging, induces immunosuppression and may lead to the development of skin cancers. On the other hand, UVR has been shown to be effective in the treatment of numerous skin diseases and thus, various phototherapy modalities have been developed to date. Narrow-band ultraviolet B (NB-UVB) emitting a light with a peak around 311 nm has been demonstrated to be effective in the treatment of various skin disorders; currently it is one of the most commonly used phototherapy devices. Despite NB-UVB has been developed more than 30 years ago, the exact mechanism of its therapeutic action remains poorly understood. To date, most of NB-UVB effects were attributed to its influence on immune cells; however, nearly 90% of NB-UVB irradiation is absorbed by epidermis and keratinocytes seem to be important players in mediating NB-UVB biological activity. Here, we have reviewed the current data about the influence of NB-UVB on epidermal cells, with a special emphasis on cell proliferation and death.

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“…Studies of broad-band UVB (BBUVB)-treated keratinocytes have demonstrated that apoptosis is a direct consequence of dose and exposure time [2][3][4] . NBUVB is also capable of inducing apoptosis in keratinocytes, though at significantly higher doses of energy than BBUVB 5 . NBUVB 1,000 mJ/cm 2 induces apoptosis in approximately 50% of keratinocytes.…”

Section: Introductionmentioning

confidence: 99%

“…In keratinocytes, secreted TGF-β1 was downregulated less than 50% by UVA, while there was no significant alteration by UVB after irradiation with 20 and 40 J/cm 2 and 10 and 20 mJ/cm 2 , respectively 10 . The effect of TGF-β isoforms on keratinocytes during NBUVB irradiation at a dose that causes apoptosis has not been well-defined 5 . In the present study, we investigate the differences in TGF-β isoform expression in keratinocytes after exposure to NBUVB.…”

Section: Introductionmentioning

confidence: 99%

Differential Expression of TGF-β Isoforms in Human Kerationocytes by Narrow Band UVB

et al. 2008

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Background: Transforming growth factor-β (TGF-β), a multifunctional growth factor, has three isoforms: TGF-β1, TGF-β2, and TGF-β3. Different isoforms of TGF-β are associated with different proliferation and differentiation states of the epidermis. Narrow band ultraviolet B (NBUVB) emits a concentrated UVB source of 311 nm. NBUVB 1,000 mJ/cm 2 induces apoptosis in approximately 50% of keratinocytes.Objective: The purpose of this study was to evaluate whether irradiation with NBUVB would alter the expression and production of TGF-β1, 2, and 3.Methods: We measured TGF-β1, 2, and 3 mRNA and TGF-β1 and 2 protein levels at 800, 1,000, and 1,200 mJ/cm 2 for 24 hours and 48 hours. Results: TGF-β1 mRNA levels were increased at both 24 hr and 48 hr, TGF-β2 mRNA levels were decreased at both 24 hr and 48 hr, and TGF-β3 mRNA levels were increased at 24 hr and similar to control at 48 hr. TGF-β1 protein levels were increased at 48 hr but decreased at 24 hr. TGF-β2 protein levels were decreased at both 24 hr and 48 hr.

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Narrow-band UVB induces apoptosis in human keratinocytes

Cited by 60 publications

References 30 publications

The translational revolution and use of biologics in patients with inflammatory skin diseases

The translational revolution and use of biologics in patients with inflammatory skin diseases

Effects of Narrow Band UVB (311 nm) Irradiation on Epidermal Cells

Differential Expression of TGF-β Isoforms in Human Kerationocytes by Narrow Band UVB

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