Narrative of a versatile and adept species Pseudomonas putida

Kivisaar, Maia

doi:10.1099/jmm.0.001137

Cited by 34 publications

(19 citation statements)

References 227 publications

(286 reference statements)

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“…The soil and root‐colonizing bacterium Pseudomonas putida KT2440 is growingly receiving attention as a suitable chassis for a suite of biotechnological and environmental applications (Nikel et al ., 2016; Nikel and de Lorenzo, 2018; Aparicio et al ., 2019; Kivisaar, 2020). In this context, the characterization of the mechanisms that trigger the swim or attach decision are decisive for, e.g.…”

Section: Introductionmentioning

confidence: 99%

The Wsp intermembrane complex mediates metabolic control of the swim‐attach decision of Pseudomonas putida

Hueso-Gil

Calles

2020

Environmental Microbiology

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Summary Pseudomonas putida is a microorganism of biotechnological interest that—similar to many other environmental bacteria—adheres to surfaces and forms biofilms. Although various mechanisms contributing to the swim‐attach decision have been studied in this species, the role of a 7‐gene operon homologous to the wsp cluster of Pseudomonas aeruginosa—which regulates cyclic di‐GMP (cdGMP) levels upon surface contact—remained to be investigated. In this work, the function of the wsp operon of P. putida KT2440 has been characterized through inspection of single and multiple wsp deletion variants, complementation with Pseudomonas aeruginosa's homologues, combined with mutations of regulatory genes fleQ and fleN and removal of the flagellar regulator fglZ. The ability of the resulting strains to form biofilms at 6 and 24 h under three different carbon regimes (citrate, glucose and fructose) revealed that the Wsp complex delivers a similar function to both Pseudomonas species. In P. putida, the key components include WspR, a protein that harbours the domain for producing cdGMP, and WspF, which controls its activity. These results not only contribute to a deeper understanding of the network that regulates the sessile‐planktonic decision of P. putida but also suggest strategies to exogenously control such a lifestyle switch.

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Section: Introductionmentioning

confidence: 99%

The Wsp intermembrane complex mediates metabolic control of the swim‐attach decision of Pseudomonas putida

Hueso-Gil

Calles

2020

Environmental Microbiology

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“…P. putida is also routinely used as a host in industrial biocatalysis. Readers are referred to the recent review of Kivisaar (2020) for more information about these aspects. The P. putida group includes also clinical isolates.…”

Section: Pseudomonas Gessardii Subgroupmentioning

confidence: 99%

The use of Pseudomonas spp. as bacterial biocontrol agents to control plant diseases

Höfte¹

2021

Burleigh Dodds Series in Agricultural Science

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Most Pseudomonas biocontrol strains are associated with the rhizosphere of plants, where they control soil pathogens by antibiosis or competition, and leaf pathogens via induced systemic resistance. Genome mining and the division of the vastly heterogeneous genus Pseudomonas in phylogenomic (sub)groups has clarified the relation between biocontrol characteristics and phylogeny. Based on their activity, Pseudomonas biocontrol strains come in three types. A first type, represented by P. chlororaphis, P. protegens, P. corrugata and P. aeruginosa (sub)group strains, produces an arsenal of secondary metabolites with broad antimicrobial activity. The second type is found in the P. putida, P. fluorescens, P. koreensis, P. mandelii, and P. gessardii (sub)group. The spectrum of biocontrol properties of these strains is less diverse and involves siderophores and cyclic lipopeptides. The third type colonizes above-ground plant parts. Strains from this type mainly belong to the P. syringae group and are used to control postharvest pathogens. This chapter starts with recent advances in Pseudomonas taxonomy and a summary of its most important biocontrol traits. It then provides an overview of the most important Pseudomonas groups and subgroups harboring biocontrol strains. Examples of well-characterized and representative biocontrol strains show the links between phylogeny, ecology and biocontrol traits. The chapter concludes by reviewing commercially-available biocontrol strains.

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“…Pseudomonas putida is a ubiquitous Gram-negative bacterium used for biotechnological and bioremediation applications 34-37 . Strain KT2440, for instance, is a promising microbial chassis for handling the synthesis of difficult-to-produce chemicals involving harsh reactions and complex biochemistries 36-40 . Alas, metabolic engineering of P. putida still relies largely on trial-and-error approaches.…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporal manipulation of the mismatch repair system ofPseudomonas putidaaccelerates phenotype emergence

Fernández-Cabezón

Cros

Nikel

2021

Preprint

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Developing complex phenotypes in industrially-relevant bacteria is a major goal of metabolic engineering, which encompasses the implementation of both rational and random approaches. In the latter case, several tools have been developed towards increasing mutation frequencies—yet the precise spatiotemporal control of mutagenesis processes continues to represent a significant technical challenge. Pseudomonas species are endowed with one of the most efficient DNA mismatch repair (MMR) systems found in bacteria. Here, we investigated if the endogenous MMR system could be manipulated as a general strategy to artificially alter mutation rates in Pseudomonas species. To bestow a conditional mutator phenotype in the platform bacterium Pseudomonas putida, we constructed inducible mutator devices to modulate the expression of the dominant-negative mutLE36K allele. Regulatable overexpression of mutLE36K in a broad-host-range, easy-to-cure plasmid format resulted in a transitory inhibition of the MMR machinery, leading to a significant increase (up to 438-fold) in mutation frequencies and a heritable fixation of genome mutations. Following such accelerated mutagenesis-followed-by selection approach, three phenotypes were successfully evolved: resistance to antibiotics streptomycin and rifampicin and reversion of a synthetic uracil auxotrophy. Thus, these mutator devices could be applied to accelerate evolution of metabolic pathways in long-term evolutionary experiments, alternating cycles of (inducible) mutagenesis coupled to selection schemes.

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Narrative of a versatile and adept species Pseudomonas putida

Cited by 34 publications

References 227 publications

The Wsp intermembrane complex mediates metabolic control of the swim‐attach decision of Pseudomonas putida

The Wsp intermembrane complex mediates metabolic control of the swim‐attach decision of Pseudomonas putida

The use of Pseudomonas spp. as bacterial biocontrol agents to control plant diseases

Spatiotemporal manipulation of the mismatch repair system ofPseudomonas putidaaccelerates phenotype emergence

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