Naringinase Biosynthesis by Aspergillus niger on an Optimized Medium Containing Red Grapefruit Albedo

Abstract

This study aimed to develop a method of naringinase biosynthesis by Aspergillus niger KMS on an optimized culture medium. The concentration of the six medium components in shake flasks was optimized by the Box and Wilson factor gradient method. Naringinase’s substrate, naringin, powdered albedo, flavedo, and red grapefruit segment membranes were used to stimulate naringinase biosynthesis. Rhamnose was chosen as the carbon source, while the nitrogen source was yeast extract and sodium nitrate. Naringinase biosynthesis was most favorable in the culture medium with the following composition (g 100 mL): 3.332—NaNO3; 3.427—yeast extract; 0.184—KH2PO4; 0.855—red grapefruit albedo; 0.168—naringin; 2.789—rhamnose. The obtained Aspergillus niger KMS culture fluid was concentrated, thereby precipitating the protein. As a result, a naringinase preparation with high activity, equal to 816 µmol × min−1 × g−1, was obtained.

Neurospora sp. Mediated Synthesis of Naringenin for the Production of Bioactive Nanomaterials

“…Among the fungal genera, Aspergillus has been extensively explored for naringinase production, with approximately nine species of Aspergillus and Penicillium and Rhizopus sp. reported for this purpose [41]. Although, the fungus is a good source for naringinase production, a significant drawback is that the time required for growth and production of biomolecules could be days or sometimes months.…”