Naringin suppressing the PI3K/Akt signaling pathway as a drug target in BIU87 cells

Abstract

Background: Flavonoids are regarded as potential anti-cancer drugs due to their toxicity and anti-proliferation functions. Naringin, which is a kind of flavanone compounds targeting anti-tumor treatment, has been focused on reliving several types of cancers, besides of bladder cancer. This paper was aimed to investigate whether and how naringin induced bladder cancer cells. Method: MTT, cell-cycle, transwell and cell apoptosis were used to assess the cellular responses. Molecular expressions and the signaling pathway of naringin inducing BIU87 cells were evaluated by western blot and real-time quantitative PCR. Results: It was found that naringin effectively suppressed proliferation and invasion of BIU87 cells by downregulating the expression of PI3K and its downstream pathway. The corresponding proteins related to viability and motility, including CDK2, Bcl-2 and Fascin, were inhibited. Conclusion: It can be concluded that naringin targeting to PI3K in bladder tumor cells expressed abundantly and inhibited the growth and invasive ability of tumor cells.