Naringin Promotes Skeletal Muscle Fiber Remodeling by the AdipoR1-APPL1-AMPK Signaling Pathway

Abstract

Naringin, a natural flavonoid mainly found in citrus fruit, has been reported to exert a positive effect on improving skeletal muscle health. However, the effects and potential mechanisms of naringin on skeletal muscle fiber switching is still unclear. Here, we discovered that oral administration of naringin increased the low-speed running time, four-limb hanging time, body oxygen consumption in mice, enhanced aerobic enzyme activity, MyHC I expression, and slow-twitch fiber percentage in mice skeletal muscle. By contrast, naringin decreased α-GPDH enzyme activity, MyHC IIb expression, and fast-twitch fiber percentage. Moreover, naringin increased the concentration of serum adiponectin and activated the expression of AdipoR1, APPL1, AMPK, and PGC-1α. Furthermore, by the in vitro experiment and AdipoR1 knockdown, we found that inhibition of the AdipoR1 signaling pathway significantly reduced the effect of naringin on slow-twitch fiber-/fast-twitch fiber-related gene and protein expression. In conclusion, our results indicated that naringin could induce skeletal muscle fiber transition from fast twitch to slow twitch via the AdipoR1 signaling pathway. This study may provide new strategy for improving exercise endurance and slow muscle fiber deficiency-related diseases.

Punicalagin protects against impaired skeletal muscle function in high-fat-diet-induced obese mice by regulating TET2

“…40 Higher expression levels of MYH1, MYH2a, and MYH7b have been linked to increased muscle strength and mass, thereby achieving the purpose of enhancing skeletal muscle function and quality. 41 Moreover, polyphenol supplementation could increase the expression levels of MYH1 and MYH2a and thereby increase the proportion of type I and IIA fibers in mice. 42,43 In the present study, we found that the expression levels of MYH1, MYH2a, and MYH7b were significantly higher in the PUN group than in the HFD group, suggesting that PUN could increase the proportion of type I and IIA fibers (Fig.…”

A Lignan from Alnus japonica Activates Myogenesis and Alleviates Dexamethasone-induced Myotube Atrophy

“…These findings suggest that DFS might be an alternative therapeutic agent for preventing and managing muscle atrophy. Recent studies have reported that natural compounds from plants such as resveratrol, naringin, and myricetin mediate muscle fiber transformation from slow-to-fast fiber type or vice versa 8 , 9 , 10 , 33 .…”

Sesamin Activates Skeletal Muscle FNDC5 Expression and Increases Irisin Secretion via the SIRT1 Signaling Pathway

“…The low-speed and high-speed running tests were carried out as described in previous studies. 29,30 The velocity of each group mice in high-speed test was increased by 5 m/min until 40 m/ min (every 2 min) and the velocity in low-speed test was increased by 1 m/min until 40 m/min (every 3 min).…”

“…Briefly, after 24 h acclimation of the system, the physical activity of mice was evaluated over the next 48 h by measuring the running or movements in the X -, Y - and Z -axes of metabolic chamber. , Mice’s muscle strength and exercise ability were tested by using the rat/mouse grip strength meter (SA417, Jiangsu Science Biotechnology Co., Ltd., Jiangsu, China) and treadmill (ZH-PT, KaiHuiSD Technology Co., Ltd., Beijing, China). The low-speed and high-speed running tests were carried out as described in previous studies. , The velocity of each group mice in high-speed test was increased by 5 m/min until 40 m/min (every 2 min) and the velocity in low-speed test was increased by 1 m/min until 40 m/min (every 3 min).…”