Naringenin-loaded nano-structured lipid carrier fortifies oxaliplatin-dependent apoptosis in HT-29 cell line

Raeisi, Sina; Chavoshi, Hadi; Mohammadi, Maryam; Ghorbani, Marjan; Sabzichi, Mehdi; Ramezani, Fatemeh

doi:10.1016/j.procbio.2019.05.013

Cited by 50 publications

(17 citation statements)

References 36 publications

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“…It was found that these compounds largely inhibited the growth of MCF-7, SK-BR-3, and ZR-75-1 cells in a dose-dependent manner [ 58 ]. Naringenin has been applied as an original treatment for cancer, as it suppresses cell growth and induces cell apoptosis of HT-29 colon cancer [ 59 ]. It was reported that epicatechin was effectively used to inhibit the growth of different cancer cells, such as those of prostate cancer, papilloma, and colorectal cancer [ 60 ].…”

Section: Resultsmentioning

confidence: 99%

Nanostructured Thin Coatings Containing Anthriscus sylvestris Extract with Dual Bioactivity

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Grumezescu

et al. 2020

Molecules

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Plant extracts are highly valuable pharmaceutical complexes recognized for their biological properties, including antibacterial, antifungal, antiviral, antioxidant, anticancer, and anti-inflammatory properties. However, their use is limited by their low water solubility and physicochemical stability. In order to overcome these limitations, we aimed to develop nanostructured carriers as delivery systems for plant extracts; in particular, we selected the extract of Anthriscus sylvestris (AN) on the basis of its antimicrobial effect and antitumor activity. In this study, AN-extract-functionalized magnetite (Fe3O4@AN) nanoparticles (NPs) were prepared by the co-precipitation method. The purpose of this study was to synthesize and investigate the physicochemical and biological features of composite coatings based on Fe3O4@AN NPs obtained by matrix-assisted pulsed laser evaporation technique. In this respect, laser fluence and drop-casting studies on coatings were performed. The physical and chemical properties of laser-synthesized coatings were investigated by scanning electron microscopy, while Fourier transform infrared spectroscopy comparative analysis was used for determining the chemical structure and functional integrity. Relevant data regarding the presence of magnetic nanoparticles as the only crystalline phase and the size of nanoparticles were obtained by transmission electron microscopy. The in vitro toxicity assessment of the Fe3O4@AN showed significant cytotoxic activity against human adenocarcinoma HT-29 cells after prolonged exposure. Antimicrobial results demonstrated that Fe3O4@AN coatings inhibit microbial colonization and biofilm formation in clinically relevant bacteria species and yeasts. Such coatings are useful, natural, and multifunctional solutions for the development of tailored medical devices and surfaces.

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Section: Resultsmentioning

confidence: 99%

Nanostructured Thin Coatings Containing Anthriscus sylvestris Extract with Dual Bioactivity

Neguț

Grumezescu

et al. 2020

Molecules

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“…Another study investigated the effect of NRG–loaded NLCs on apoptosis induction in colon cancer cells (HT-29), along with the administration of oxaliplatin ( Figure 4 ) [ 114 ]. NLCs are the second generation of lipid-based nanoparticles found about seven years after the SLNs [ 107 ].…”

Section: Nrg Nano-scaled Delivery Systemsmentioning

confidence: 99%

“…According to the results of this research, NRG loaded into NLCs could successfully decrease the cell growth of HT-29 colon cancer. NRG-NLCs increase anticancer drugs’ effectiveness, reduce the side effects of chemotherapy treatments and along with chemotherapy drugs, and stimulate apoptosis [ 114 ].…”

Section: Nrg Nano-scaled Delivery Systemsmentioning

confidence: 99%

Naringenin Nano-Delivery Systems and Their Therapeutic Applications

et al. 2021

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Naringenin (NRG) is a polyphenolic phytochemical belonging to the class of flavanones and is widely distributed in citrus fruits and some other fruits such as bergamot, tomatoes, cocoa, and cherries. NRG presents several interesting pharmacological properties, such as anti-cancer, anti-oxidant, and anti-inflammatory activities. However, the therapeutic potential of NRG is hampered due to its hydrophobic nature, which leads to poor bioavailability. Here, we review a wide range of nanocarriers that have been used as delivery systems for NRG, including polymeric nanoparticles, micelles, liposomes, solid lipid nanoparticles (SLNs), nanostructured lipid carriers (NLCs), nanosuspensions, and nanoemulsions. These nanomedicine formulations of NRG have been applied as a potential treatment for several diseases, using a wide range of in vitro, ex vivo, and in vivo models and different routes of administration. From this review, it can be concluded that NRG is a potential therapeutic option for the treatment of various diseases such as cancer, neurological disorders, liver diseases, ocular disorders, inflammatory diseases, skin diseases, and diabetes when formulated in the appropriate nanocarriers.

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“…Naringenin has very limited water solubility (46 ± 6.0 μg/mL), gastrointestinal degradation, and low bioavailability, thus providing a major hurdle for its development as a drug and limiting its therapeutic use [ 12 ]. Several approaches have been undertaken to increase its solubility using nanotechnology [ 13 , 14 , 15 , 16 , 17 ]. Kumar et al synthesized chitosan-encapsulated naringenin nanoparticles with antioxidant and anticancer activities [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Formulation Design, Statistical Optimization, and In Vitro Evaluation of a Naringenin Nanoemulsion to Enhance Apoptotic Activity in A549 Lung Cancer Cells

et al. 2020

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Naringenin (NAR), a flavonoid mainly found in citrus and grapefruits, has proven anti-cancer activities. However, the poor water solubility and low bioavailability of NAR limits its use as a therapeutic agent. The aim of this study was to develop and optimize stable naringenin nanoemulsions (NAR-NE) using a Box–Behnken experimental design to obtain a formulation with a higher efficiency. Anticancer activity of optimized NAR-NE was evaluated in A549 lung cancer cells using cell viability, flow-cytometric assays, and enzyme-linked immunosorbent assay. The stabilized nanoemulsion, which showed a spherical surface morphology, had a globule size of 85.6 ± 2.1 nm, a polydispersity index of 0.263 ± 0.02, a zeta potential of −9.6 ± 1.2 mV, and a drug content of 97.34 ± 1.3%. The NAR release from the nanoemulsion showed an initial burst release followed by a stable and controlled release for a longer period of 24 h. The nanoemulsion exhibited excellent thermodynamic and physical stability against phase separation and storage. The NAR-NE showed concentration-dependent cytotoxicity in A549 lung cancer cells, which was greater than that of free NAR. The percentage of apoptotic cells and cell cycle arrest at the G2/M and pre-G1 phases induced by NAR-NE were significantly higher than those produced by free NAR (p < 0.05). NAR-NEs were more effective than the NAR solution in reducing Bcl2 expression, while increasing pro-apoptotic Bax and caspase-3 activity. Therefore, stabilized NAR-NE could be a suitable drug delivery system to enhance the effects of NAR in the treatment of lung cancer.

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Naringenin-loaded nano-structured lipid carrier fortifies oxaliplatin-dependent apoptosis in HT-29 cell line

Cited by 50 publications

References 36 publications

Nanostructured Thin Coatings Containing Anthriscus sylvestris Extract with Dual Bioactivity

Nanostructured Thin Coatings Containing Anthriscus sylvestris Extract with Dual Bioactivity

Naringenin Nano-Delivery Systems and Their Therapeutic Applications

Formulation Design, Statistical Optimization, and In Vitro Evaluation of a Naringenin Nanoemulsion to Enhance Apoptotic Activity in A549 Lung Cancer Cells

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