Naringenin Eye Drops Inhibit Corneal Neovascularization by Anti-Inflammatory and Antioxidant Mechanisms

Oguido, Ana Paula Miyagusko Taba; Hohmann, Miriam S. N.; Pinho‐Ribeiro, Felipe A.; Crespigio, Jefferson; Domiciano, Talita P.; Verri, Waldiceu A.; Casella, Antônio Marcelo Barbante

doi:10.1167/iovs.16-19702

Cited by 40 publications

(25 citation statements)

References 67 publications

(95 reference statements)

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“…For example, Sun et al explored cell cycle and apoptosis of corneal neovascularization based on the alkali-burn injured rat model [17]. The alkali-burn injured rat model of corneal neovascularization was used to identify the inflammatory and oxidative mechanism [18]. Additionally, the alkali-burn injured rat model of corneal injury was also taken to explore the retinal damage [19], the therapeutic effects of antagomir-21 [20] and the inflammatory fibrosis [21].…”

Section: Discussionmentioning

confidence: 99%

lncRNA MIAT suppression alleviates corneal angiogenesis through regulating miR-1246/ACE

et al. 2019

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Corneal neovascularization (CRNV) is a prevalence eye disorder that affects the transparency and refraction properties of eyes. To explore the correlation between the level of Angiotensin II (Ang II) and corneal angiogenesis, the rat model of CRNV was established using alkali-burn, while the human umbilical vein endothelial cells (HUVECs) were stimulated using VEGF to induce the CRNV cells in vitro. RNA immunoprecipitation (RIP) and RNA pull-down were performed to validate the relationship between MIAT and miR-1246. The expression of MIAT and Ang II was increased, while miR-1246 was decreased in CRNV rat model. VEGF stimulation significantly promoted cell proliferation and migration of HUVECs, knockdown of MIAT dramatically reversed the effects of VEGF, while cells co-transfected with miR-1246 inhibitor obviously abolished the effect of VEGF+si-MIAT, however, enalaprilat abolished the effects of VEGF+si-MIAT+miR-1246 inhibitor. MIAT directly regulated the expression of miR-1246. In conclusion, VEGF stimulation promoted cell proliferation and migration of HUVECs mainly through regulating MIAT/miR-1246/ACE. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

lncRNA MIAT suppression alleviates corneal angiogenesis through regulating miR-1246/ACE

et al. 2019

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“…Its effects on inflammation have been demonstrated in UVB irradiation [61,124], inflammatory pain [58,62,125], and arthritis [59]. The mechanisms by which naringenin reduces inflammation and pain are related to inhibition of oxidative stress [61,62,124,126], leukocyte recruitment [59,60], MPO activity [124,127], NF-κB activation [58][59][60], mRNA expression of inflammasome components [59], pro-inflammatory cytokine production (TNF-α, IL-1β, IL-33, IL-6, IFN-γ, IL-12, IL-4, IL-5, IL-13, IL-17, and IL-22) [58][59][60]62,124,125,127], and MAPK signaling activation [128]. In addition, naringenin modulates macrophages activation [129] and microbicidal activity of neutrophils [130], and reduces DC maturation [131].…”

Section: Flavanonesmentioning

confidence: 99%

Therapeutic Potential of Flavonoids in Pain and Inflammation: Mechanisms of Action, Pre-Clinical and Clinical Data, and Pharmaceutical Development

et al. 2020

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Pathological pain can be initiated after inflammation and/or peripheral nerve injury. It is a consequence of the pathological functioning of the nervous system rather than only a symptom. In fact, pain is a significant social, health, and economic burden worldwide. Flavonoids are plant derivative compounds easily found in several fruits and vegetables and consumed in the daily food intake. Flavonoids vary in terms of classes, and while structurally unique, they share a basic structure formed by three rings, known as the flavan nucleus. Structural differences can be found in the pattern of substitution in one of these rings. The hydroxyl group (-OH) position in one of the rings determines the mechanisms of action of the flavonoids and reveals a complex multifunctional activity. Flavonoids have been widely used for their antioxidant, analgesic, and anti-inflammatory effects along with safe preclinical and clinical profiles. In this review, we discuss the preclinical and clinical evidence on the analgesic and anti-inflammatory proprieties of flavonoids. We also focus on how the development of formulations containing flavonoids, along with the understanding of their structure-activity relationship, can be harnessed to identify novel flavonoid-based therapies to treat pathological pain and inflammation.

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“…[30] Migration of endothelial cells begins on the second and third days of the inflammation, and this process is completed by the sixth or seventh day. [31] The fibrinopurulent exudates fill anastomotic space in peritoneal cavity. This fibrinopurulent exudates repress collagen and new blood vessel formation and, as a result, that the anastomosis heals by secondary intention.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the effects of two different marine-derived omega- 3 fatty acid sources, krill oil and fish oil, on the healing of primary colonic anastomoses after colectomy applied Wistar albino rat model

Ferhatoğlu

Kıvılcım

Vural

et al. 2019

Ulus Travma Acil Cerrahi Derg

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BACKGROUND: Oils from marine organisms have a different fatty acid composition. Fish oil (FO) has a high content of eicosapentaenoic and docosahexaenoic acids esterified to triacylglycerols; while in krill oil (KO), fatty acids are primarily esterified to phospholipids. This study aimed to compare the efficacy of two different, marine-derived omega-3 fatty acid sources in the wound healing of colon anastomoses rat model. METHODS: For the study, we used 42 male Wistar albino rats. The rats were divided into six groups with seven rats in each group-CO3: left colonic anastomosis (control group), sacrificed on the third day; KO3: left colonic anastomosis + oral KO, sacrificed on the third day; FO3: left colonic anastomosis + oral FO, sacrificed on the third day; CO7: left colonic anastomosis (control group), sacrificed on the seventh day; KO7: left colonic anastomosis + oral KO, sacrificed on the seventh day; FO7: left colonic anastomosis + oral FO, sacrificed on the seventh day. Peritoneal adhesions, anastomotic bursting pressures, hydroxyproline levels, and histological examination of the anastomotic tissue were evaluated. RESULTS: On day 7, bursting pressure and hydroxyproline measurements of the KO group was significantly higher than the FO group (p=0.012; p=0.002, respectively). Also, on day 7, a statistically significant difference was observed between the groups according to inflammatory cell infiltration, fibroblast activity, neoangiogenesis, and collagen deposition in favor of the KO group (p=0.023; p=0.028; p=0.016; p=0.012, respectively). CONCLUSION: Both KO and FO supplementation in patients before colorectal surgery may reduce some risk of anastomotic leakage; and KO might be a better alternative and excellent omega-3 source.

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Naringenin Eye Drops Inhibit Corneal Neovascularization by Anti-Inflammatory and Antioxidant Mechanisms

Abstract: Collectively, these results indicate that naringenin eye drops are protective in alkali-induced corneal burn by inhibiting leukocyte recruitment, the proangiogenic factor expression, inflammatory cytokine production, and loss of antioxidant defenses.

Cited by 40 publications

References 67 publications

lncRNA MIAT suppression alleviates corneal angiogenesis through regulating miR-1246/ACE

lncRNA MIAT suppression alleviates corneal angiogenesis through regulating miR-1246/ACE

Therapeutic Potential of Flavonoids in Pain and Inflammation: Mechanisms of Action, Pre-Clinical and Clinical Data, and Pharmaceutical Development

Comparison of the effects of two different marine-derived omega- 3 fatty acid sources, krill oil and fish oil, on the healing of primary colonic anastomoses after colectomy applied Wistar albino rat model

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