Naringenin, a flavanone inhibits the proliferation of cerebrally implanted C6 glioma cells in rats

Sabarinathan, Devan; Palani, Mahalakshmi; Vanisree, Arambakkam Janardhanam

doi:10.1016/j.cbi.2010.09.028

Cited by 43 publications

(35 citation statements)

References 46 publications

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“…In addition, naringenin is known to suppress various NF‐κB‐regulated gene products including vascular endothelial growth factor (VEGF), the proinflammatory enzyme cyclooxigenase 2 (COX2), matrix metalloproteinase‐2 and matrix metalloproteinase‐9 (MMP‐2 and MMP‐9), which are associated with tumour progression and metastasis. Several studies on human cancer and animal tumour model have documented that increased tumour expression of COX‐2, VEGF, MMP‐2 and MMP‐9 was correlated with more aggressive lesions that sustain inflammation, tumour growth, progression and metastasis. Consistent with earlier findings, angiogenic effects of VEGF seen in the present study are also mediated via the upregulation of MMPs that degrade the components of the extracellular matrix (ECM) during metastatic dissemination of tumour cells promote tumour invasion and metastasis .…”

Section: Discussionmentioning

confidence: 99%

Expression of Concern: Attenuation of NDEA‐induced hepatocarcinogenesis by naringenin in rats

Subramanian

Arul

2012

Cell Biochemistry & Function

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Chemoprevention is one of the most promising and realistic approaches in the prevention of cancer. Several bioactive compounds present in fruits and vegetables have revealed their cancer curative potential on hepatocellular carcinoma. Naringenin is one such naturally occurring flavonoid widely found in citrus fruits. In this study, we examined the molecular mechanisms by which naringenin inhibited NDEA-induced hepatocellular carcinoma in rats by analysing the expression patterns of proliferating cell nuclear antigen, Bcl-2, NF-κB, VEGF and MMP-2/9. Enhanced cell proliferation and apoptotic evasion in NDEA-induced hepatocarcinogenesis was associated with imbalance in pro-apoptotic and anti-apoptotic proteins together with upregulation of proliferating cell nuclear antigen (PCNA) and downregulation of caspase-3. Administration of pretreatment and posttreatment of naringenin decreased the expression of PCNA and Bcl-2 and increased the expression of Bax and caspase-3, indicating antiproliferative and apoptotic effects, respectively. Administration of NDEA increased the tumour expression of NF-κB, COX-2, VEGF, MMP-2 and MMP-9 that was correlated with more aggressive lesions and tumour growth. Downregulation of NF-κB, VEGF and MMPs by naringenin seen in the present study were correlated with the inhibition of liver tumour induced by NDEA. Our results suggest that naringenin could act as a legitimate agent by inhibiting cancer processes.

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Section: Discussionmentioning

confidence: 99%

Expression of Concern: Attenuation of NDEA‐induced hepatocarcinogenesis by naringenin in rats

Subramanian

Arul

2012

Cell Biochemistry & Function

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“…Naringenin triggered the C6 glioma cells cerebrally implanted in rat to undergo apoptosis by upregulating connexin 43, reducing the Bcl‐2/Bax ratio, and increasing the activities of caspase‐3 and ‐9 . Administering naringenin also attenuated the expression of protein kinase C, cyclin D1, and cyclin‐dependent protein kinase 4, which in turn suppressed the proliferation of cerebrally implanted C6 glioma cells in rats . Stompor et al reported that treatment with naringenin at 150, 250, and 500 μM for 24 hours reduced the cell viability to 60%, 50%, and below 20%, respectively, in GBM U‐118 MG cells.…”

Section: Discussionmentioning

confidence: 99%

Naringenin inhibited migration and invasion of glioblastoma cells through multiple mechanisms

Chen

Chang

Wang

et al. 2018

Environmental Toxicology

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Glioblastoma (GBM) is the most mortality brain cancer in the world. Due to high invasion and drug resistance cause the poor prognosis of GBM. Naringenin, an ingredient of citrus, exhibits many cellular functions such as antioxidant, anti-inflammation, and anticancer. Naringenin inhibits the migration of bladder and lung cancer via modulation of MMP-2 and/or MMP-9 activities, Naringenin inhibits migration and trigger apoptosis in gastric cancer cells through downregulation of AKT pathway. However, the effects of naringenin in GBM still remain to be elucidated. In this study, we reveal the molecular mechanisms of naringenin in the inhibition of migration and invasion in GBM. No overt alternation of cell proliferation was found in of GBM 8901 cells treated with different concentration of naringenin.Slight decreased cell viability was found in GBM 8401 cell treated with 200 and 300 μM naringenin.Significant reduction of migration and invasion as assayed by Boyden chamber analysis was found in of GBM cells treated with 100, 200, and 300 μM naringenin. Zymography analysis also revealed that the activities of MMP-2 and MMP-9 of GBM cells were significantly inhibited in response to 100, 200, or 300 μM naringenin treatment. Proteins of MMP-2 and MMP-9 were downregulated in naringenin treated GBM cells. In addition, naringenin also attenuated the activities of ERK and p38.Naringenin decreased mesenchymal markers (snail and slug) expression as revealed by Western blot analysis. Taken together, our findings indicated that naringenin eliminated the migration and invasion of GBM cells through multiple mechanisms including inhibition of MMPs, ERK, and p38 activities and modulation of EMT markers. Our results also suggested that naringenin may be a potential agent to prevent metastasis of GBM.

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“…The scheme of naringenin administration is due to its increasing availability in plasma immediately after it is ingested (Wang et al, 2014b; Mata‐Bilbao Mde et al, 2007). The dose was defined according to its neuroprotective action reported in the literature (Raza et al, 2013; Sabarinathan et al, 2011).…”

Section: Methodsmentioning

confidence: 99%

Effect of maternal antioxidant supplementation and/or exercise practice during pregnancy on postnatal overnutrition induced by litter size reduction: Brain redox homeostasis at weaning

August

Maurmann

Saccomori

et al. 2018

Intl J of Devlp Neuroscience

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Prenatal and early postnatal environments can permanently influence health throughout life. Early overnutrition increases the risk to develop chronic diseases. Conversely, the intake of flavonoids and exercise practice during pregnancy seem to promote long-term benefits to offspring. We hypothesized that benefic interventions during pregnancy could protect against possible postnatal neurochemical alterations caused by overnutrition induced by reduced litter size. Female Wistar rats were divided into four groups: (1) sedentary + vehicle, (2) sedentary + naringenin, (3) swimming exercise + vehicle, and (4) swimming exercise + naringenin. One day after birth, the litter was culled to 8 pups (control) or 3 pups (overfed) per dam, yielding control and overfed subgroups for each maternal group. Serum of 21-days-old pups was collected, also the cerebellum, hippocampus, and hypothalamus were dissected. Litter size reduction increased fat mass and enhanced body weight. Maternal interventions, when isolated, caused reduced glucose serum levels in offspring nurtured in control litters. In the cerebellum, reducing the litter size decreased the activity of thioredoxin reductase, which was prevented by maternal supplementation with naringenin. Hippocampus and hypothalamus have shown altered antioxidant enzymes activities in response to litter size reduction. Interestingly, when maternal exercise and naringenin supplementation were allied, the effect disappeared, suggesting a concurrent effect of the two maternal interventions. In conclusion, exercise or naringenin supplementation during pregnancy can be important interventions for combating the increasing rates of overweight during the infancy and its related neurochemical changes, especially when applied isolated.

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Naringenin, a flavanone inhibits the proliferation of cerebrally implanted C6 glioma cells in rats

Cited by 43 publications

References 46 publications

Expression of Concern: Attenuation of NDEA‐induced hepatocarcinogenesis by naringenin in rats

Expression of Concern: Attenuation of NDEA‐induced hepatocarcinogenesis by naringenin in rats

Naringenin inhibited migration and invasion of glioblastoma cells through multiple mechanisms

Effect of maternal antioxidant supplementation and/or exercise practice during pregnancy on postnatal overnutrition induced by litter size reduction: Brain redox homeostasis at weaning

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