Narcotic mechanisms of acute toxicity of chlorinated anilines in Folsomia candida (Collembola) revealed by gene expression analysis

Janssens, Thierry; Giesen, Daniel; Mariën, Janine; Straalen, Nico M. van; Gestel, Cornelis A.M. van; Roelofs, Dick

doi:10.1016/j.envint.2011.03.012

Cited by 17 publications

(5 citation statements)

References 75 publications

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“…For this species, standardized protocols are available for soil-quality assessment [13]. Various studies have demonstrated that F. candida is suitable in classical soil toxicity tests as well as in toxicogenomics studies [6,8,14,15]. The present study aimed to correlate the chemical composition of soil samples from a contaminated site with ecotoxicity tests and transcriptomic analysis to reach a better insight into how toxicogenomics can be used to evaluate soil quality.…”

Section: Introductionmentioning

confidence: 99%

Integrating transcriptomics into triad‐based soil‐quality assessment

Chen¹,

Boer²,

Wagelmans

et al. 2014

Enviro Toxic and Chemistry

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The present study examined how transcriptomics tools can be included in a triad-based soil-quality assessment to assess the toxicity of soils from riverbanks polluted by metals. To that end, the authors measured chemical soil properties and used the International Organization for Standardization guideline for ecotoxicological tests and a newly developed microarray for gene expression in the indicator soil arthropod Folsomia candida. Microarray analysis revealed that the oxidative stress response pathway was significantly affected in all soils except one. The data indicate that changes in cell redox homeostasis are a significant signature of metal stress. Finally, 32 genes showed significant dose-dependent expression with metal concentrations. They are promising genetic markers providing an early indication of the need for higher-tier testing of soil quality. During the bioassay, the toxicity of the least polluted soils could be removed by sterilization. The gene expression profile for this soil did not show a metal-related signature, confirming that a factor other than metals (most likely of biological origin) caused the toxicity. The present study demonstrates the feasibility and advantages of integrating transcriptomics into triad-based soil-quality assessment. Combining molecular and organismal life-history trait stress responses helps to identify causes of adverse effects in bioassays. Further validation is needed for verifying the set of genes with dose-dependent expression patterns linked with toxic stress.

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Section: Introductionmentioning

confidence: 99%

Integrating transcriptomics into triad‐based soil‐quality assessment

Chen¹,

Boer²,

Wagelmans

et al. 2014

Enviro Toxic and Chemistry

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“…Toxicogenomics has been selected as an experimental approach as it enables unbiased, global profiling of the molecular biological response space (Richard, 2006;Edwards and Preston, 2008), without preselection of response parameters. For single chemicals, it has been shown that exposure results in chemical-specific, gene expression profiles (Hamadeh et al, 2002;Yang et al, 2007;Kiyosawa et al, 2010;Janssens et al, 2011;Piña and Barata, 2011;Meganathan et al, 2012;Theunissen et al, 2012). The hypothesis tested in the present study is whether specific MoAs have common gene expression profiles that can be used to classify compounds and whether compounds with multiple MoAs can be classified and distinguished from those with only one MoA.…”

Section: Introductionmentioning

confidence: 93%

“…To this end, two aniline compounds and PCP were included which are suggested to have a polar narcotic MoA, as well as ethylenglycol, which has a narcotic MoA. Structure-based MoA assignments generate equivocal results concerning the separation between polar narcosis and uncoupling (Schüürmann et al, 1996), with some studies classifying the anilines and PCP as uncouplers, while other studies classifying them as polar narcotics (Russom et al, 1997;Schüürmann et al, 1997;Argese et al, 2001;Spycher et al, 2008a;Janssens et al, 2011;Dom et al, 2012). Since these test chemicals are not unequivocally classified as uncouplers, the TMRM assay could not be used for the selection of equipotent test concentrations.…”

Section: Moa Specific Gene Expression Profile At Cytotoxic Based Concmentioning

confidence: 99%

Mode of Action Assignment of Chemicals Using Toxicogenomics: A Case Study with Oxidative Uncouplers

Hawliczek-Ignarski

Cenijn²,

Legler³

et al. 2017

Front. Environ. Sci.

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A criterion frequently used to group chemicals in risk assessment is "mode of toxic action" (MoA). Routinely, structure-based approaches are used for the MoA categorization of chemicals, but they can produce conflicting results or fail to classify compounds. Biological activity-based approaches such as toxicogenomics which provide an unbiased overview of the transcriptomic changes after exposure to a compound may complement structure-based approaches in MoA assignment. Here, we investigate whether toxicogenomic profiles as generated after in vitro exposure of an established cell line (C3A hepatoma cells) are able to group together chemicals with an uncoupling MoA, and to distinguish the uncouplers from chemicals with other MoAs. In a first step, we examined whether chemicals sharing the same uncoupling of oxidative phosphorylation (OXPHOS) MoA produce similar toxicogenomic profiles and can be grouped together. In a next step, we tested whether the toxicogenomic profiles discriminate between OXPHOS and chemicals displaying a (polar) narcotic MoA. Experimentally, cells were exposed in vitro to equipotent concentrations of the test compounds and gene expression profiles were measured. The resulting toxicogenomic profiles assigned OXPHOS to one cluster and discriminated between the OXPHOS and the (polar) narcotics. In addition, the toxicogenomics data revealed that one and the same chemical can display multiple MoAs, which may help to explain conflicting results of MoA classification from structure-based approaches. The results strongly suggest the feasibility of MoA grouping of chemicals by using in vitro cell assay-based toxicogenomic profiles.

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“…Collembola (springtails) are a group with high abundance in almost any environment, and Folsomia candida has emerged as a standard species and genomic model organism for soil-toxicology 15 . Several studies have addressed the effects of different stressors, such as metals 16 , 17 , pesticides 18 , 19 or combined pollutants 20 , in gene expression of this collembolan species over the last decade. Since then, considerable advances have been made to assemble a more complete transcriptome of F .…”

Section: Introductionmentioning

confidence: 99%

An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide

Simões

Novais

Natal-da-Luz

et al. 2018

Sci Rep

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The use of integrative molecular approaches can aid in a comprehensive understanding of the effects of toxicants at different levels of biological organization, also supporting risk assessment. The present study aims to unravel the toxicity mechanisms of a widely used herbicide to the arthropod Folsomia candida exposed in a natural soil, by linking effects on reproduction, proteomics and genome-wide gene expression. The EC50 effects on reproduction over 4 weeks was 4.63 mg glyphosate/kg of soil. The formulation included a polyethoxylated tallowamine as an adjuvant, which at 50% effect on reproduction had an estimated concentration of 0.87–1.49 mg/kg of soil. No effects were observed on survival and reproduction when using the isolated active substance, pointing the toxicity of the formulated product to the co-formulant instead of the active ingredient, glyphosate. RNA sequencing and shotgun proteomics were applied to assess differential transcript and protein expressions between exposed and control organisms in time, respectively. Specific functional categories at protein and transcriptome levels were concordant with each other, despite overall limited correlations between datasets. The exposure to this formulation affected normal cellular respiration and lipid metabolism, inducing oxidative stress and leading to impairment in biological life cycle mechanisms such as molting and reproduction.

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Narcotic mechanisms of acute toxicity of chlorinated anilines in Folsomia candida (Collembola) revealed by gene expression analysis

Cited by 17 publications

References 75 publications

Integrating transcriptomics into triad‐based soil‐quality assessment

Integrating transcriptomics into triad‐based soil‐quality assessment

Mode of Action Assignment of Chemicals Using Toxicogenomics: A Case Study with Oxidative Uncouplers

An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide

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