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Berndsen, Marta Rós; Guðbjartsson, Tómas; Berndsen, F

doi:10.17992/lbl.2019.09.247

Cited by 36 publications

(21 citation statements)

References 39 publications

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“…During the initial stages, PINK1 phosphorylates Ser65 of Ub (pSer65-Ub) to recruit autoinhibited cytosolic Parkin. [18][19][20] The pSer65-Ub-Parkin interaction stimulates Ub ligase activity, resulting in the formation of ubiquitin chains, which also serve as substrates of PINK1 to further retain and activate Parkin. Another consequence of the pSer65-Ub-Parkin interaction is that it releases the N-terminal Ub-like (Ubl) domain from the core of Parkin, increasing the rate at which PINK1 phosphorylates Ser65 of the Ubl domain.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Middle-Down MS Analysis of Parkin-Mediated Ubiquitin Chain Assembly

Deol

Eyles

Strieter

2020

J. Am. Soc. Mass Spectrom.

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Misregulation of the E3 ubiquitin ligase Parkin and the kinase PINK1 underlie both inherited and idiopathic Parkinson's disease-associated neurodegeneration. Parkin and PINK1 work together to catalyze the assembly of ubiquitin chains on substrates located on the outer mitochondrial membrane to facilitate autophagic removal of damaged mitochondria through a process termed mitophagy. Quantitative measurements of Parkin-mediated chain assembly, both in vitro and on mitochondria, have revealed that chains are composed of Lys6, Lys11, Lys48, and Lys63 linkages. The combinatorial nature of these chains is further expanded by the ability of PINK1 to phosphorylate individual subunits. The precise architecture of chains produced by the coordinated action of PINK1 and Parkin, however, are unknown. Here, we demonstrate that quantitative middle-down mass spectrometry using uniformly 15 N-labeled ubiquitin variants as internal standards informs on the extent of chain branching. We find that Parkin is a prolific branching enzyme in vitro. Quantitative middle-down mass spectrometry also reveals that phospho-Ser65ubiquitin (pSer65-Ub)-a key activator of Parkin-is not incorporated into chains to a significant extent. Our results suggest that Parkin-mediated chain branching is "on-pathway", and branch points are the principal targets of the deubiquitinase USP30.

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Section: Introductionmentioning

confidence: 99%

Quantitative Middle-Down MS Analysis of Parkin-Mediated Ubiquitin Chain Assembly

Deol

Eyles

Strieter

2020

J. Am. Soc. Mass Spectrom.

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“…Complications of laparoscopic inguinal hernia repair include pain in the operative site and groin area, postoperative respiratory depression, postoperative nausea and vomiting after anesthesia, postoperative urinary retention, bladder injury, and scrotal hematoma, among which the scrotal or groin hematoma is the most common, with an incidence of 0.5% to 78%. [12] This study found that the incidence of respiratory depression and nausea and vomiting in patients after laparoscopic inguinal hernia repair was about 8%, and there was no statistical significance among all groups (P > .05), as shown in Table 5. In this study, a total of 1 case of surgical site hematoma occurred, and the patient was in group B.…”

Section: Adverse Eventsmentioning

confidence: 73%

Effect of ketorolac tromethamine combined with dezocine prior administration on hemodynamics and postoperative analgesia in patients undergoing laparoscopic hernia repair

Wu¹,

Cai²,

Li³

et al. 2022

Medicine

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Objective: To observe the effect of Ketorolac tromethamine combined with dezocine prior administration on hemodynamics and postoperative sedation in patients undergoing laparoscopic hernia repair. Methods: 100 male patients aged 60 to 80 years old, a line to elective laparoscopic inguinal hernia repair, were randomly divided into four groups: control group (Group A) and dezocine group (Group B), ketorolac tromethamine group (Group C), ketorolac tromethamine combined with dezocine group (Group D). Patients were administrated with 0.1 mg/kg dezocine in Group B, 0.5 mg/kg ketorolac in Group C, 0.1 mg/kg dezocine, and 0.5 mg/kg ketorolac in Group D, and with an equal dose of normal saline in group A. The heart rate (HR) and mean arterial pressure (MAP) of patients in 4 groups were recorded at each time point as follows, T0 (enter the operating room), T1 (before skin resection), 10 min after pneumoperitoneum (T2), mesh placement (T3), and laryngeal mask extraction (T4). Operation time, awakening time (time from drug withdrawal to consciousness recovery), the dosage of propofol, sufentanil, remifentanil, and intraoperative vasoactive drug dosage were recorded to compare. Visual analog scale score and sedation Ramsay score were evaluated 1, 6, 12, and 24 hours after extubation. Results: There was no significant difference in operation time, anesthesia recovery time, sufentanil dosage, and vasoactive drugs among all groups. The amount of propofol in Group B and D was less than that in Group A and C ( P < .05), and there was no difference between Group B and D, A and C ( P > .05). The amount of remifentanil in Group B, C, and D was less than that in Group A ( P < .05), and Group D was less than B and C ( P < .05). After extubation, HR and MAP were significantly higher than before ( P < .05). Compared with T0, HR and MAP increased in each group at T4, but MAP and HR in Group D increased the least ( P < .05). There were significant differences between Group B, C, D, and A, MAP and HR fluctuated little during extubation ( P < .05), but there was a significant difference between Group D and B, C ( P < .05). Visual analog scale scores of Group B, C, and D were lower than those of A at 1, 6, and 12 hours after surgery ( P < .05), and there was a significant difference between Group D, and B, C ( P < .05). Ramsay scores in Group B and D were higher than those in A and C at 1 and 6 hours after the operation ( P < .05). There was no difference in the incidence of adverse reactions among groups. Conclusion: The prophylactic use of ketorolac tromethamine and dezocine before laparoscopic inguinal hernia repair can reduc...

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“…Inguinal hernias are the most frequent type of hernia and their repair is among the most common procedures general surgeons perform. 1 Various risk factors can cause a predisposition to the development of hernias, such as male sex, old age, a high body mass index, connective tissue disorders and activity that increases intra-abdominal pressure, like chronic coughing or weight-lifting. 2 Inguinal hernias are divided into two categories depending on the point of protrusion of the tissue.…”

Section: Introductionmentioning

confidence: 99%

A Rare Case of a Direct Incarcerated Inguinal Hernia Containing an Epiploic Appendage and a Literature Review

Papamichail

Karlafti

Malliou

et al. 2023

Sultan Qaboos Univ Med J

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Inguinal hernias are a widespread condition, responsible for a large number of acute abdomen cases. Typically, indirect, rather than direct, hernias are the ones leading to complications, as a consequence of their narrower hernial defect. Our case concerns a rather rare incidence of a direct incarcerated hernia in a 71-year-old man who presented with acute pain in the left inguinal area. Upon clinical examination, an irreducible inguinal mass was palpated. Therefore, the existence of a complicated hernia was suspected. The patient underwent an emergency repair, during which it was established that the hernia was direct and incarcerated and that its sac contained an ischemic epiploic appendage. The hernia was successfully repaired with mesh, the patient recovered uneventfully and was discharged five days later. Despite the rarity of complicated direct inguinal hernias, they should always be included in the differential diagnosis of irreducible groin masses, because they can increase severe complications. Keywords: Direct inguinal hernia; Appendix epiploica.

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Nárakviðslit - Yfirlitsgrein

Cited by 36 publications

References 39 publications

Quantitative Middle-Down MS Analysis of Parkin-Mediated Ubiquitin Chain Assembly

Quantitative Middle-Down MS Analysis of Parkin-Mediated Ubiquitin Chain Assembly

Effect of ketorolac tromethamine combined with dezocine prior administration on hemodynamics and postoperative analgesia in patients undergoing laparoscopic hernia repair

A Rare Case of a Direct Incarcerated Inguinal Hernia Containing an Epiploic Appendage and a Literature Review

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