Napins, 2S albumins, are major allergens in oilseed rape and turnip rape

Puumalainen, T.; Poikonen, S.; Kotovuori, A.; Vaali, Kirsi; Kalkkinen, Nisse; Reunala, Timo; Turjanmaa, Kristiina; Palosuo, Timo

doi:10.1016/j.jaci.2005.10.004

Cited by 51 publications

(31 citation statements)

References 36 publications

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“…Allergenic proteins, especially the 2S proteins of Brassicaceae seeds including canola can end up in cold-pressed canola oil (Poikonen et al, 2008;Puumalainen et al, 2006). Napin is a gastro-intestinal allergen of yellow mustard and rarely reported for inflicting fatal anaphylactic reactions (Monsalve et al, 2001).…”

Section: Allergenicity Of Canola Proteinsmentioning

confidence: 99%

“…Napin is a gastro-intestinal allergen of yellow mustard and rarely reported for inflicting fatal anaphylactic reactions (Monsalve et al, 2001). Among the proteins that are capable of eliciting immunogenic response from B. napus and B. rapa seed extracts in a skin prick test, Bra n 1 (Napin BnIII, napin nIII or napin 3; P80208, 2SS3_BRANA) and Bra r 1(Q42473, BRACM; UniProtKB/Swiss-Prot entry) were prominent (Poikonen et al, 2008;Puumalainen et al, 2006). Presence of four S-S bonds allows tight packing and formation of a compact structure of napin.…”

Section: Allergenicity Of Canola Proteinsmentioning

confidence: 99%

See 1 more Smart Citation

Canola/rapeseed protein-functionality and nutrition

Wanasundara¹,

McIntosh²,

Perera³

et al. 2016

OCL

136

103

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-Protein rich meal is a valuable co-product of canola/rapeseed oil extraction. Seed storage proteins that include cruciferin (11S) and napin (2S) dominate the protein complement of canola while oleosins, lipid transfer proteins and other minor proteins of non-storage nature are also found. Although oil-free canola meal contains 36-40% protein on a dry weight basis, non-protein components including fibre, polymeric phenolics, phytates and sinapine, etc. of the seed coat and cellular components make protein less suitable for food use. Separation of canola protein from non-protein components is a technical challenge but necessary to obtain full nutritional and functional potential of protein. Process conditions of raw material and protein preparation are critical of nutritional and functional value of the final protein product. The storage proteins of canola can satisfy many nutritional and functional requirements for food applications. Protein macromolecules of canola also provide functionalities required in applications beyond edible uses; there exists substantial potential as a source of plant protein and a renewable biopolymer. Available information at present is mostly based on the protein products that can be obtained as mixtures of storage protein types and other chemical constituents of the seed; therefore, full potential of canola storage proteins is yet to be revealed.Keywords: Canola / rapeseed storage proteins / cruciferin / napin / protein digestibility / functional properties Résumé -Protéines de canola et de colza : fonctionnalités et nutrition. Les tourteaux riches en protéines repré-sentent un coproduit de valeur de l'extraction de l'huile de canola/colza. Dans la graine, les protéines de stockage, notamment la cruciférine (11S) et la napine (2S), dominent la fraction protéique du canola, mais des oléosines, des protéines de transfert de lipides et d'autres protéines mineures non dédiées au stockage sont également présentes. Bien que le tourteau de canola déshuilé contienne 36-40 % de protéines sur poids sec, la présence de composants non protéiques, dont les fibres, les polymères phénoliques, les phytates, la sinapine, etc. issus de l'enveloppe de la graine et des composants cellulaires rendent les protéines moins appropriées à une utilisation en alimentation humaine. Cette revue présente les connaissances actuelles en termes de valeur nutritionnelle et fonctionnelle des protéines issues des graines de canola. La séparation des protéines de canola des composants non protéiques représente un défi technique mais nécessaire pour libérer totalement le potentiel nutritionnel et fonctionnel de la protéine. Les protéines de stockage de canola peuvent satisfaire un grand nombre d'exigences nutritionnelles et fonctionnelles pour des applications alimentaires. Les macromolécules protéiques de canola offrent également des fonctionnalités requises dans les applications dépassant les seules utilisations alimentaires ; un potentiel important existe en tant que source de protéines végétales et de biopol...

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Section: Allergenicity Of Canola Proteinsmentioning

confidence: 99%

Section: Allergenicity Of Canola Proteinsmentioning

confidence: 99%

Canola/rapeseed protein-functionality and nutrition

Wanasundara¹,

McIntosh²,

Perera³

et al. 2016

OCL

136

103

View full text Add to dashboard Cite

show abstract

“…oleifera , Bra n 1) and turnip rape ( Brassica rapa ssp. oleifera , Bra r 1) [93]. It is also important to highlight the fact that not all the 2S albumins should be considered major allergens.…”

Section: Clinical Relevance and Cross- Reactivity Of 2s Allergensmentioning

confidence: 99%

2S Albumin Storage Proteins: What Makes them Food Allergens?

Moreno¹,

Clemente²

2008

Open Biochem J

196

210

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2S albumin storage proteins are becoming of increasing interest in nutritional and clinical studies as they have been reported as major food allergens in seeds of many mono- and di-cotyledonous plants. This review describes the main biochemical, structural and functional properties of these proteins thought to play a role in determining their potential allergenicity. 2S albumins are considered to sensitize directly via the gastrointestinal tract (GIT). The high stability of their intrinsic protein structure, dominated by a well-conserved skeleton of cysteine residues, to the harsh conditions present in the GIT suggests that these proteins are able to cross the gut mucosal barrier to sensitize the mucosal immune system and/or elicit an allergic response. The flexible and solvent-exposed hypervariable region of these proteins is immunodominant and has the ability to bind IgE from allergic patients´ sera. Several linear IgE-binding epitopes of 2S albumins spanning this region have been described to play a major role in allergenicity; the role of conformational epitopes of these proteins in food allergy is far from being understood and need to be investigated. Finally, the interaction of these proteins with other components of the food matrix might influence the absorption rates of immunologically reactive 2S albumins but also in their immune response.

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“…In this context, oilseed rape is not considered a common allergenic food (EC, 2007), although rare cases of occupational allergy to inhaled dust/flour and sensitisation and allergic reactions in atopic patients have been reported (Monsalve et al, 1997;Suh et al, 1998;Poikonen et al, 2006;Puumalainen et al, 2006).…”

Section: Assessment Of Allergenicity Of the Whole Gm Plantmentioning

confidence: 99%

Scientific Opinion on application (EFSA-GMO-BE-2010-81) for the placing on the market of genetically modified herbicide-tolerant oilseed rape Ms8, Rf3 and Ms8 × Rf3 for food containing or consisting of, and food produced from or containing ingredients pro

2012

EFSA Journal

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This scientific opinion is a risk assessment for the placing on the market of the genetically modified (GM) herbicide‐tolerant oilseed rape (OSR) Ms8, Rf3 and Ms8×Rf3 for food containing or consisting of, and food produced from or containing ingredients produced from, these GM plants. OSR Ms8 (male sterile) and Rf3 (fertility restorer) are the parents of OSR Ms8×Rf3, which is fertile, contains the bar, barstar and barnase genes, and is tolerant to glufosinate‐ammonium‐containing herbicides. Integrity of the inserts present in the single events was demonstrated in the stack. Molecular characterisation did not reveal any safety issues. No biologically relevant differences were identified in the composition or agronomic and phenotypic characteristics of OSR Ms8×Rf3, as compared with its non‐GM comparator, except for the newly expressed proteins. The safety assessment identified no concerns regarding the potential toxicity and allergenicity of OSR Ms8, Rf3 and Ms8×Rf3. A broiler study confirmed that OSR Ms8×Rf3 is as nutritious as its non‐GM comparator. There are no indications of an increased likelihood of establishment and spread of feral OSR Ms8, Rf3 and Ms8×Rf3 plants, or of hybridising wild relatives, unless exposed to glufosinate‐ammonium‐containing herbicides. Considering the intended uses, potential interactions of feral OSR Ms8, Rf3 and Ms8×Rf3 plants with the biotic and abiotic environment are not considered an issue. Environmental risks associated with a possible horizontal transfer into bacteria have not been identified. The monitoring plan and reporting intervals are in line with the intended uses. In conclusion, the EFSA GMO Panel considers that the information available for OSR Ms8, Rf3 and Ms8×Rf3 addresses the scientific comments raised by Member States and that OSR Ms8, Rf3 and Ms8×Rf3 are unlikely to have an adverse effect on human and animal health or on the environment, in the context of their intended uses.

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Napins, 2S albumins, are major allergens in oilseed rape and turnip rape

Cited by 51 publications

References 36 publications

Canola/rapeseed protein-functionality and nutrition

Canola/rapeseed protein-functionality and nutrition

2S Albumin Storage Proteins: What Makes them Food Allergens?

Scientific Opinion on application (EFSA-GMO-BE-2010-81) for the placing on the market of genetically modified herbicide-tolerant oilseed rape Ms8, Rf3 and Ms8 × Rf3 for food containing or consisting of, and food produced from or containing ingredients pro

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