Naphthol Blue Black and 99mTc-Labeled Mannosylated Human Serum Albumin (99mTc-MSA) Conjugate as a Multimodal Lymph Node Mapping Nanocarrier

Abstract

99mTc-labeled mannosylated human serum albumin (MSA) has been reported as a sentinel lymph node (SLN)-imaging agent by binding to macrophages in the LNs. By conjugating it with blue dye, we developed a new multimodal radio-nanocarrier by visual investigation, fluorescence imaging, and single photon emission computed tomography (SPECT)/computed tomography (CT). Binding affinities of seven blue dyes to MSA were tested. According to the spectroscopic study and visual inspection of MSA-bound dyes, naphthol blue black (NBB) was selected as the best candidate of multimodal agent. Thus, 99mTc-MSA-NBB conjugate was prepared and further investigated using mice. After footpad injection, it showed high popliteal LN accumulation at 1 h. SPECT/CT also showed high popliteal as well as inguinal LN uptakes at 10 min that sustained until 2 h. In conclusion, we prepared a multimodal SLN imaging radio-nanocarrier, 99mTc-MSA-NBB conjugate, and confirmed its excellency as a multimodal probe for SLN mapping.

Albumin Nanoparticle-Based Drug Delivery Systems

“…99mTc-labeled Mannose-based HSA (MSA), which binds to a blue dye, has been reported as an SLN imaging agent by binding to macrophages in LNs. 253 Indocyanine green loaded HSA (ICG-HSA) nanoparticles which radiolabeled with technetium-99 m (99mTc) were prepared to locate SLNs and inhibit tumor metastasis. The photothermal treatment of SLN enhanced the inhibitory effect on lung metastasis in mice and significantly prolonged their survival time.…”

Transforming Nuclear Medicine with Nanoradiopharmaceuticals

“…Albumin nanoparticles loaded with 99m Tc have been used for SPECT/CT imaging applications, such as imaging of tumors, sentinel lymph node, arterial dispersal, pulmonary delivery, liver functions, etc. Ji and co-workers have prepared a tumor-targeted formulation of doxorubicin-loaded and 131 I labeled BSA nanoparticles .…”