Nanozymes: created by learning from nature

Zhang, Ruofei; Fan, Kelong; Yan, Xiyun

doi:10.1007/s11427-019-1570-7

Cited by 140 publications

(93 citation statements)

References 84 publications

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“…Nanozymes refer to nanoparticle‐based enzyme mimics, and they have attracted extensive interest recently due to their low cost, high stability and versatile catalytic activities . Nanoceria is an interesting and important nanozyme, and it has been studied mainly for its redox activity to mimic oxidase, peroxidase, catalase, and superoxide dismutase (SOD) .…”

Section: Introductionmentioning

confidence: 99%

Self‐limited Phosphatase‐mimicking CeO₂ Nanozymes

Liu

2020

ChemNanoMat

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CeO2 nanoparticles or nanoceria is a very interesting enzyme mimic (nanozyme) with a diverse range of catalytic activities. Most of the previous studies focused on its redox chemistry for mimicking oxidase, peroxidase, and catalase‐like activities, and as a scavenger of reactive oxygen species. Considering CeO2 has both Ce(III) and Ce(IV) species and both interact strongly with inorganic phosphate, nanoceria has also been studied for its phosphatase‐like activity. We herein compared these species along with alkaline phosphatase (ALP). First, CeO2 and Ce(IV) have good activity using p‐nitrophenylphosphate (p‐NPP) as a substrate, while other metal ions failed to show this activity. Since a reaction product is inorganic phosphate and we observed an interesting enzyme kinetic profile, the effect of phosphate was studied, which inhibited both CeO2 and ALP. The inhibition constant was about 5‐fold smaller for CeO2. The inhibition effect of polyphosphates was even stronger. Due to the product inhibition effect, CeO2 is unlikely to be a typical multiple turnover nanozyme, and the system is self‐limited. For the other anions, only F− showed a moderate inhibition effect on the cerium species, while adsorption of DNA did not inhibit the activity. Finally, heat treated ALP lost the activity, but CeO2 and Ce(IV) remained active. This study has deepened our understanding of CeO2 as a phosphatase mimicking nanozyme, which could be useful for biosensing and chemical biology applications.

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Section: Introductionmentioning

confidence: 99%

Self‐limited Phosphatase‐mimicking CeO₂ Nanozymes

Liu

2020

ChemNanoMat

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“…With the rapid development of nanotechnology, over 50 kinds of diverse nanomaterials have been found with different enzyme-like catalytic activity, including noble-metal nanocrystals, transition metal oxides, suldes, selenides, nitrides, and phosphides, carbon-based nanomaterials, polymer-metal complexes and metal-organic frameworks (MOFs). [20][21][22][23][24][25][26][27][28][29][30] Most of which belong to oxidoreductase-and hydrolases-mimics, such as oxidase, peroxidase, catalase, superoxide dismutase, nuclease, phosphatase and so forth. Moreover, the inherent physicochemical properties (magnetic, optical, thermal and electrical properties) of these nanomaterials integrated with the unique enzyme-like activities will endow them with extensive potential compared to natural enzymes.…”

Section: Introductionmentioning

confidence: 99%

Atomic engineering of single-atom nanozymes for enzyme-like catalysis

et al. 2020

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“…[ 17 ] Compared with natural proteic enzymes, nanozymes possess a number of unique advantages, particularly, the high stability and low cost for scale up. [ 18 ] Of note, most nanozymes exhibit activities of oxidoreductase, including oxidase (OXD), peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD). Furthermore, it has been shown that these multiple enzyme‐like activities can be constructed into one nanozyme and tuned by nanostructural and surface modification.…”

Section: Introductionmentioning

confidence: 99%

A Nanozyme‐Based Artificial Peroxisome Ameliorates Hyperuricemia and Ischemic Stroke

Zhang

Wang

et al. 2020

Adv Funct Materials

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154

104

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Artificial peroxisome has drawn a lot of attentions for its usefulness in fabricating protocell system and great potential in treating diseases. However, it is still a significant challenge to prepare a practicable artificial peroxisome to complement multiple and stable functions under physiological condition. Herein, a novel strategy is reported to design an artificial peroxisome using a nanozyme to accommodate multiple enzyme‐like activities that mimics those enzymes in natural peroxisome. The enzymatic active sites are introduced into graphitized moieties on the shell of a hollow carbon nanozyme by doping iron and nitrogen to form Fe–N4 coordination and atomic Fe cluster. With Fe clusters as reversible cofactors and Fe–N4 as prosthetic group, the resulted carbon nanozyme exhibits stable and multiple peroxisomal‐like activities, including catalase, uricase, superoxide dismutase, peroxidase, and oxidase, which is referred as nanozyme‐based artificial peroxisome (pero‐nanozysome). This pero‐nanozysome shows therapeutic effect for treating hyperuricemia and protecting neurons from free radicals generated during ischemic stroke by employing the tandem activities of uricase‐catalase and superoxide‐dismutase‐catalase, respectively. This study indicates that the pero‐nanozysome is a promising candidate to design artificial peroxisome performing in vivo functions.

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Nanozymes: created by learning from nature

Cited by 140 publications

References 84 publications

Self‐limited Phosphatase‐mimicking CeO₂ Nanozymes

Self‐limited Phosphatase‐mimicking CeO₂ Nanozymes

Atomic engineering of single-atom nanozymes for enzyme-like catalysis

A Nanozyme‐Based Artificial Peroxisome Ameliorates Hyperuricemia and Ischemic Stroke

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Nanozymes: created by learning from nature

Cited by 140 publications

References 84 publications

Self‐limited Phosphatase‐mimicking CeO2 Nanozymes

Self‐limited Phosphatase‐mimicking CeO2 Nanozymes

Atomic engineering of single-atom nanozymes for enzyme-like catalysis

A Nanozyme‐Based Artificial Peroxisome Ameliorates Hyperuricemia and Ischemic Stroke

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Product

Resources

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Self‐limited Phosphatase‐mimicking CeO₂ Nanozymes

Self‐limited Phosphatase‐mimicking CeO₂ Nanozymes