Nanozyme and aptamer- based immunosorbent assay for aflatoxin B1

Wu, Long; Zhou, Min; Wang, Yasheng; Liu, Jingmin

doi:10.1016/j.jhazmat.2020.123154

Cited by 87 publications

(49 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even though, the integration of nanomaterials and traditional ELISA has achieved significant enhancements, it is only few parts of this technique which could be modified, not as a whole improvement. Because many synthesized nanomaterials are coated with the exceeded dense surfactant on the surface that may bring to obstacles to modify antibody, some challenges still remain in the application of current ELISA-based method for pathogen detection ( Wu et al, 2019 ; Wu et al, 2020 ). In addition, the design and production of antibodies is complex, resource-intensive and the assays are unsuitable for multiplexing.…”

Section: Diagnostic Methods For Plant Pathogensmentioning

confidence: 99%

Biosensor Technologies for Early Detection and Quantification of Plant Pathogens

et al. 2021

View full text Add to dashboard Cite

Plant pathogens are a major reason of reduced crop productivity and may lead to a shortage of food for both human and animal consumption. Although chemical control remains the main method to reduce foliar fungal disease incidence, frequent use can lead to loss of susceptibility in the fungal population. Furthermore, over-spraying can cause environmental contamination and poses a heavy financial burden on growers. To prevent or control disease epidemics, it is important for growers to be able to detect causal pathogen accurately, sensitively, and rapidly, so that the best practice disease management strategies can be chosen and enacted. To reach this goal, many culture-dependent, biochemical, and molecular methods have been developed for plant pathogen detection. However, these methods lack accuracy, specificity, reliability, and rapidity, and they are generally not suitable for in-situ analysis. Accordingly, there is strong interest in developing biosensing systems for early and accurate pathogen detection. There is also great scope to translate innovative nanoparticle-based biosensor approaches developed initially for human disease diagnostics for early detection of plant disease-causing pathogens. In this review, we compare conventional methods used in plant disease diagnostics with new sensing technologies in particular with deeper focus on electrochemical and optical biosensors that may be applied for plant pathogen detection and management. In addition, we discuss challenges facing biosensors and new capability the technology provides to informing disease management strategies.

show abstract

Section: Diagnostic Methods For Plant Pathogensmentioning

confidence: 99%

Biosensor Technologies for Early Detection and Quantification of Plant Pathogens

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The nanozymes-based immunosorbent assay has proved the superior performance of nanozymes in enhancing the detection sensitivity. Based on mesoporous SiO 2 /Au-Pt (m-SAP), an aptamer, and Fe 3 O 4 magnetic nanoparticles (MNP), a nanozyme and aptamer-based immunosorbent assay (NAISA) was constructed for AFB1 detection ( Wu et al, 2020a ). In the work, m-SAP were prepared to act as signal labels, aptamer was adopted to recognize AFB1, and MNP facilitated to realize magnetic separation.…”

Section: Application In Food Contaminants Detectionmentioning

confidence: 99%

Nanozyme Applications: A Glimpse of Insight in Food Safety

Zhou

Wang

et al. 2021

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

Nanozymes own striking merits, including high enzyme-mimicking activity, good stability, and low cost. Due to the powerful and distinguished functions, nanozymes exhibit widespread applications in the field of biosensing and immunoassay, attracting researchers in various fields to design and engineer nanozymes. Recently, nanozymes have been innovatively used to bridge nanotechnology with analytical techniques to achieve the high sensitivity, specificity, and reproducibility. However, the applications of nanozymes in food applications are seldom reviewed. In this review, we summarize several typical nanozymes and provide a comprehensive description of the history, principles, designs, and applications of nanozyme-based analytical techniques in food contaminants detection. Based on engineering and modification of nanozymes, the food contaminants are classified and then discussed in detail via discriminating the roles of nanozymes in various analytical methods, including fluorescence, colorimetric and electrochemical assay, surface-enhanced Raman scattering, magnetic relaxing sensing, and electrochemiluminescence. Further, representative examples of nanozymes-based methods are highlighted for contaminants analysis and inhibition. Finally, the current challenges and prospects of nanozymes are discussed.

show abstract

“…Apts are categorised into two categories: oligonucleotides and peptide molecules. Oligonucleotide-based Apts capture target molecules via complementary shapes and electrostatic and hydrophobic interactions, whereas peptide Apts consist mainly of variable peptide domains that bind to a protein scaffold at both terminal ends [18,34,35]. The term "aptamer" was derived from the word "aptus" in Latin, which means "to fit" and it was used to name the nucleic acid-based (RNA) ligands that were selected with the newly developed systematic evolution of ligands by exponential enrichment (SELEX) technique in 1990 [34].…”

Section: Aptamersmentioning

confidence: 99%

“…Alternatively, the enzyme-linked immunosorbent assay (ELISA) approach offers a convenient route to filter out any contaminated samples from within a large batch of samples. Nevertheless, in ELISA, the possibility of false positives is high due to both the matrix effect and the fact that the antibodies are often highly unstable [ 17 , 18 ].…”

Section: Conventional and Advanced Analytical Technologiesmentioning

confidence: 99%

Recent Advances in Conventional Methods and Electrochemical Aptasensors for Mycotoxin Detection

Ong

Pike

Tan

2021

Foods

View full text Add to dashboard Cite

The presence of mycotoxins in foodstuffs and feedstuffs is a serious concern for human health. The detection of mycotoxins is therefore necessary as a preventive action to avoid the harmful contamination of foodstuffs and animal feed. In comparison with the considerable expense of treating contaminated foodstuffs, early detection is a cost-effective way to ensure food safety. The high affinity of bio-recognition molecules to mycotoxins has led to the development of affinity columns for sample pre-treatment and the development of biosensors for the quantitative analysis of mycotoxins. Aptamers are a very attractive class of biological receptors that are currently in great demand for the development of new biosensors. In this review, the improvement in the materials and methodology, and the working principles and performance of both conventional and recently developed methods are discussed. The key features and applications of the fundamental recognition elements, such as antibodies and aptamers are addressed. Recent advances in aptasensors that are based on different electrochemical (EC) transducers are reviewed in detail, especially from the perspective of the diagnostic mechanism; in addition, a brief introduction of some commercially available mycotoxin detection kits is provided.

show abstract

Nanozyme and aptamer- based immunosorbent assay for aflatoxin B1

Cited by 87 publications

References 37 publications

Biosensor Technologies for Early Detection and Quantification of Plant Pathogens

Biosensor Technologies for Early Detection and Quantification of Plant Pathogens

Nanozyme Applications: A Glimpse of Insight in Food Safety

Recent Advances in Conventional Methods and Electrochemical Aptasensors for Mycotoxin Detection

Contact Info

Product

Resources

About