Nanozyme: An emerging alternative to natural enzyme for biosensing and immunoassay

Wang, Qingqing; Wei, Hui; Zhang, Zhiquan; Wang, Erkang; Dong, Shaojun

doi:10.1016/j.trac.2018.05.012

“…Nanomaterials with enzyme‐like characteristics have got increasingly attention in nanoresearch . To our best knowledge, the progress and achievements of the nanomaterials‐based nanozymes have been thoroughly reviewed in previous literatures . These reviews all introduced different enzyme‐like activity of nanomaterials and their different applications: enzyme‐like characteristics such as catalase‐like activity, superoxide oxidase‐like activity, ribonuclease‐like activity, oxidase‐like activity, and peroxidase‐like activity; applications such as anti‐inflammatory effects, antioxidants, neuroprotection, promotion of stem cell growth, H 2 O 2 detection, glucose detection, DNA detection, immunoassays, and pollutant removal.…”

Section: Peroxidase‐like Activity Of Nanomaterialsmentioning

confidence: 99%

Peroxidase‐Like Activity of Smart Nanomaterials and Their Advanced Application in Colorimetric Glucose Biosensors

Liu

¹

,

Huang

²

,

Qin

³

et al. 2019

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Diabetes is a dominating health issue with 425 million people suffering from the disease worldwide and 4 million deaths each year. To avoid further complications, the diabetic patient blood glucose level should be strictly monitored despite there being no cure for diabetes. Colorimetric biosensing has attracted significant attention because of its low cost, simplicity, and practicality. Recently, some nanomaterials have been found that possess unexpected peroxidase‐like activity, and great advances have been made in fabricating colorimetric glucose biosensors based on the peroxidase‐like activity of these nanomaterials using glucose oxidase. Compared with natural horseradish peroxidase, the nanomaterials exhibit flexibility in structure design and composition, and have easy separation and storage, high stability, simple preparation, and tunable catalytic activity. To highlight the significant progress in the field of nanomaterial‐based peroxidase‐like activity, this work discusses the various smart nanomaterials that mimic horseradish peroxidase and its mechanism and development history, and the applications in colorimetric glucose biosensors. Different approaches for tunable peroxidase‐like activity of nanomaterials are summarized, such as size, morphology, and shape; surface modification and coating; and metal doping and alloy. Finally, the conclusion and challenges facing peroxidase‐like activity of nanomaterials and future directions are discussed.

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“…Enzymes are highly efficient biocatalysts that can selectively and specifically catalyze various reactions under mild conditions,a nd they have been used widely in disease diagnosis and cancer treatment. [1,2] However,t heir intrinsic limitations,s uch as high cost, low stability,a nd difficulties in recovery and storage,h ave inspired the exploration and development of enzyme mimics.Nanomaterials with enzymelike characteristics were recently discovered, which were defined as nanozymes by Weia nd Wang in 2013. [3] Fe 3 O 4 nanoparticles (NPs) with intrinsic peroxidase-like activity were proposed in 2007,; [4] subsequently more than ad ozen nanomaterials with enzyme-like characteristics were reported covering transition metals,n oble metals,a nd carbon nanomaterials.…”

Section: Introductionmentioning

confidence: 99%

When Nanozymes Meet Single‐Atom Catalysis

Jiao

¹

,

Yan

²

,

Wu

³

et al. 2019

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Nanomaterials with enzyme‐like activities, coined nanozymes, have been researched widely as they offer unparalleled advantages in terms of low cost, superior activity, and high stability. The complex structure and composition of nanozymes has led to extensive investigation of their catalytic sites at an atomic scale, and to an in‐depth understanding of the biocatalysis occurring. Single‐atom catalysts (SACs), characterized by atomically dispersed active sites, have provided opportunities for mimicking metalloprotease and for bridging the gap between natural enzymes and nanozymes. In this Minireview, we illustrate the unique properties of nanozymes and we discuss recent advances in the synthesis, characterization, and applications of SACs. Subsequently, we outline the impressive progress made in single‐atom nanozymes and we discuss their applications in sensing, degradation of organic pollutants, and in therapeutic roles. Finally, we present the major challenges and opportunities remaining for a successful marriage of nanozymes and SACs.

show abstract

“…Enzymes are highly efficient biocatalysts that can selectively and specifically catalyze various reactions under mild conditions, and they have been used widely in disease diagnosis and cancer treatment . However, their intrinsic limitations, such as high cost, low stability, and difficulties in recovery and storage, have inspired the exploration and development of enzyme mimics.…”

Section: Introductionmentioning

confidence: 99%

When Nanozymes Meet Single‐Atom Catalysis

Jiao

¹

,

Yan

²

,

Wu

³

et al. 2019

View full text Add to dashboard Cite

Nanomaterials with enzyme‐like activities, coined nanozymes, have been researched widely as they offer unparalleled advantages in terms of low cost, superior activity, and high stability. The complex structure and composition of nanozymes has led to extensive investigation of their catalytic sites at an atomic scale, and to an in‐depth understanding of the biocatalysis occurring. Single‐atom catalysts (SACs), characterized by atomically dispersed active sites, have provided opportunities for mimicking metalloprotease and for bridging the gap between natural enzymes and nanozymes. In this Minireview, we illustrate the unique properties of nanozymes and we discuss recent advances in the synthesis, characterization, and applications of SACs. Subsequently, we outline the impressive progress made in single‐atom nanozymes and we discuss their applications in sensing, degradation of organic pollutants, and in therapeutic roles. Finally, we present the major challenges and opportunities remaining for a successful marriage of nanozymes and SACs.

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Nanozyme: An emerging alternative to natural enzyme for biosensing and immunoassay

Cited by 542 publications

References 65 publications

Peroxidase‐Like Activity of Smart Nanomaterials and Their Advanced Application in Colorimetric Glucose Biosensors

Peroxidase‐Like Activity of Smart Nanomaterials and Their Advanced Application in Colorimetric Glucose Biosensors

When Nanozymes Meet Single‐Atom Catalysis

When Nanozymes Meet Single‐Atom Catalysis

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