Nanowires Precisely Grown on The Ends of Microwire Electrodes Permit The Recording of Intracellular Action Potentials Within Deeper Neural Structures

Ferguson, John E.; Boldt, Chris; Puhl, Joshua G.; Stigen, Tyler; Jackson, Jadin C.; Crisp, Kevin M.; Mesce, Karen A.; Netoff, Theoden I.; Redish, A. David

doi:10.2217/nnm.11.157

Cited by 23 publications

(15 citation statements)

References 15 publications

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“…Nanofabricated devices, including nanostraws (1-3), nanowires (4-7), nanoneedles (8)(9)(10)(11)(12), and nanoelectrodes (13)(14)(15)(16)(17)(18)(19)(20)(21)(22), are increasingly being investigated as tools for cellular studies, but these structures do not readily insert through the cell membrane (2)(3)(4)(5)13,23), and assessing when (or whether) penetration has occurred is difficult due to the nanoscale features of the probe-membrane interface. To design cell-penetrating nanoprobes, a systematic approach is needed to describe nanostructure-membrane interactions at relevant temporal and spatial scales, particularly the processes of nanoprobe insertion through (3,(8)(9)(10)(11)(12)(13)15,18,24,25) or fusion with (14,16,19,(26)(27)(28) the plasma membrane.…”

Section: Introductionmentioning

confidence: 99%

Penetration of Cell Membranes and Synthetic Lipid Bilayers by Nanoprobes

Angle

Wang

Thomas

et al. 2014

Biophysical Journal

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Nanoscale devices have been proposed as tools for measuring and controlling intracellular activity by providing electrical and/or chemical access to the cytosol. Unfortunately, nanostructures with diameters of 50-500 nm do not readily penetrate the cell membrane, and rationally optimizing nanoprobes for cell penetration requires real-time characterization methods that are capable of following the process of membrane penetration with nanometer resolution. Although extensive work has examined the rupture of supported synthetic lipid bilayers, little is known about the applicability of these model systems to living cell membranes with complex lipid compositions, cytoskeletal attachment, and membrane proteins. Here, we describe atomic force microscopy (AFM) membrane penetration experiments in two parallel systems: live HEK293 cells and stacks of synthetic lipid bilayers. By using the same probes in both systems, we were able to clearly identify membrane penetration in synthetic bilayers and compare these events with putative membrane penetration events in cells. We examined membrane penetration forces for three tip geometries and 18 chemical modifications of the probe surface, and in all cases the median forces required to penetrate cellular and synthetic lipid bilayers with nanoprobes were greater than 1 nN. The penetration force was sensitive to the probe's sharpness, but not its surface chemistry, and the force did not depend on cell surface or cytoskeletal properties, with cells and lipid stacks yielding similar forces. This systematic assessment of penetration under various mechanical and chemical conditions provides insights into nanoprobe-cell interactions and informs the design of future intracellular nanoprobes.

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Section: Introductionmentioning

confidence: 99%

Penetration of Cell Membranes and Synthetic Lipid Bilayers by Nanoprobes

Angle

Wang

Thomas

et al. 2014

Biophysical Journal

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“…Engineers have enlisted the help of those who study medicinal leeches to assist in the development of new technologies for physiology research. For example, leech CNS preparations were employed to test and validate voltage-sensitive dye imaging (Miller et al 2012) and nanowire intracellular electrode fabrication methods (Ferguson et al 2012). These technologies are being adapted for use in other animal models.…”

Section: Circadian Rhythms and Clock Genesmentioning

confidence: 99%

Some recent advances in spider sensory physiology

French

Torkkeli

2015

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“…Several groups have developed novel, metal-based, microscale/nanoscale electrodes to potentially realize multi-channel intracellular recordings [19][20][21][22][23] . These electrodes successfully recorded synaptic and intracellularlike or full-blown action potentials (APs) in neuronal cultures and brain slices; however, they have not been demonstrated in vivo.…”

Section: Introductionmentioning

confidence: 99%

Engineering microscale systems for fully autonomous intracellular neural interfaces

Kumar

Baker

Okandan³

et al. 2020

Microsyst Nanoeng

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Conventional electrodes and associated positioning systems for intracellular recording from single neurons in vitro and in vivo are large and bulky, which has largely limited their scalability. Further, acquiring successful intracellular recordings is very tedious, requiring a high degree of skill not readily achieved in a typical laboratory. We report here a robotic, MEMS-based intracellular recording system to overcome the above limitations associated with form factor, scalability, and highly skilled and tedious manual operations required for intracellular recordings. This system combines three distinct technologies: (1) novel microscale, glass-polysilicon penetrating electrode for intracellular recording; (2) electrothermal microactuators for precise microscale movement of each electrode; and (3) closed-loop control algorithm for autonomous positioning of electrode inside single neurons. Here we demonstrate the novel, fully integrated system of glass-polysilicon microelectrode, microscale actuators, and controller for autonomous intracellular recordings from single neurons in the abdominal ganglion of Aplysia californica (n = 5 cells). Consistent resting potentials (<−35 mV) and action potentials (>60 mV) were recorded after each successful penetration attempt with the controller and microactuated glass-polysilicon microelectrodes. The success rate of penetration and quality of intracellular recordings achieved using electrothermal microactuators were comparable to that of conventional positioning systems. Preliminary data from in vivo experiments in anesthetized rats show successful intracellular recordings. The MEMS-based system offers significant advantages: (1) reduction in overall size for potential use in behaving animals, (2) scalable approach to potentially realize multi-channel recordings, and (3) a viable method to fully automate measurement of intracellular recordings. This system will be evaluated in vivo in future rodent studies.

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Nanowires Precisely Grown on The Ends of Microwire Electrodes Permit The Recording of Intracellular Action Potentials Within Deeper Neural Structures

Cited by 23 publications

References 15 publications

Penetration of Cell Membranes and Synthetic Lipid Bilayers by Nanoprobes

Penetration of Cell Membranes and Synthetic Lipid Bilayers by Nanoprobes

Some recent advances in spider sensory physiology

Engineering microscale systems for fully autonomous intracellular neural interfaces

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