Nanowire based bioprobes for electrical monitoring of electrogenic cells

Casanova, Adrien; Bettamin, L.; Blatché, Marie-Charline; Mathieu, Fabrice; Martin, Hélène; Gonzalez–Dunia, Daniel; Nicu, Liviu; Larrieu, Guilhem

doi:10.1088/1361-648x/aae5aa

Cited by 10 publications

(12 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The nanoelectrode array devices were fabricated in the LAAS-CNRS clean room, using a series of micro-technology processes compatible with a large-scale type of fabrication ( Casanova et al., 2018 ). Briefly, the device is composed of a cultured chamber (well) containing 60 nano-electrodes composed by 3D nanowires coated with a platinum silicide (PtSi) biocompatible surface.…”

Section: Methodsmentioning

confidence: 99%

Borna disease virus docks on neuronal DNA double-strand breaks to replicate and dampens neuronal activity

et al. 2022

View full text Add to dashboard Cite

Summary Borna disease viruses (BoDV) have recently emerged as zoonotic neurotropic pathogens. These persistent RNA viruses assemble nuclear replication centers (vSPOT) in close interaction with the host chromatin. However, the topology of this interaction and its consequences on neuronal function remain unexplored. In neurons, DNA double-strand breaks (DSB) have been identified as novel epigenetic mechanisms regulating neurotransmission and cognition. Activity-dependent DSB contribute critically to neuronal plasticity processes, which could be impaired upon infection. Here, we show that BoDV-1 infection, or the singled-out expression of viral Nucleoprotein and Phosphoprotein, increases neuronal DSB levels. Of interest, inducing DSB promoted the recruitment anew of vSPOT colocalized with DSB and increased viral RNA replication. BoDV-1 persistence decreased neuronal activity and response to stimulation by dampening the surface expression of glutamate receptors. Taken together, our results propose an original mechanistic cross talk between persistence of an RNA virus and neuronal function, through the control of DSB levels.

show abstract

Section: Methodsmentioning

confidence: 99%

Borna disease virus docks on neuronal DNA double-strand breaks to replicate and dampens neuronal activity

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Liu et al generated a 3D high-density nanowire array to record both exocellular and intracellular signals (Liu et al, 2017a). Electrical circuit models were built, as shown in (Casanova et al, 2018). It could achieve a high surface-to-volume ratio and have a large signal-to-noise ratio.…”

Section: D Mea-based Models For Ex Vivo Non Studiesmentioning

confidence: 99%

“…Nanowire electrodes under the soma entered into cells during the growth phase to record intracellular signals; electrodes near neurites were able to record exocellular signals. Casanova et al fabricated nanowire probes on a chip through a CMOS-compatible large-scale fabrication process based on conventional lithography tools ( Casanova et al, 2018 ). It could achieve a high surface-to-volume ratio and have a large signal-to-noise ratio.…”

Section: Advanced Models For Ex Vivo Non Studiesmentioning

confidence: 99%

Lab-on-Chip Microsystems for Ex Vivo Network of Neurons Studies: A Review

Zhang

Rong

Bian

2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Increasing population is suffering from neurological disorders nowadays, with no effective therapy available to treat them. Explicit knowledge of network of neurons (NoN) in the human brain is key to understanding the pathology of neurological diseases. Research in NoN developed slower than expected due to the complexity of the human brain and the ethical considerations for in vivo studies. However, advances in nanomaterials and micro-/nano-microfabrication have opened up the chances for a deeper understanding of NoN ex vivo, one step closer to in vivo studies. This review therefore summarizes the latest advances in lab-on-chip microsystems for ex vivo NoN studies by focusing on the advanced materials, techniques, and models for ex vivo NoN studies. The essential methods for constructing lab-on-chip models are microfluidics and microelectrode arrays. Through combination with functional biomaterials and biocompatible materials, the microfluidics and microelectrode arrays enable the development of various models for ex vivo NoN studies. This review also includes the state-of-the-art brain slide and organoid-on-chip models. The end of this review discusses the previous issues and future perspectives for NoN studies.

show abstract

“…PtSi has been selected as a conductive substrate for the deposition of IrO 2 . The choice of the substrate is motivated by the planned integration of IrO 2 onto vertical nanopillar arrays to be used as scalable nanoelectrodes fabricated starting from silicon nanopillars [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Plasma-Assisted Atomic Layer Deposition of IrO2 for Neuroelectronics

et al. 2023

View full text Add to dashboard Cite

In vitro and in vivo stimulation and recording of neuron action potential is currently achieved with microelectrode arrays, either in planar or 3D geometries, adopting different materials and strategies. IrO2 is a conductive oxide known for its excellent biocompatibility, good adhesion on different substrates, and charge injection capabilities higher than noble metals. Atomic layer deposition (ALD) allows excellent conformal growth, which can be exploited on 3D nanoelectrode arrays. In this work, we disclose the growth of nanocrystalline rutile IrO2 at T = 150 °C adopting a new plasma-assisted ALD (PA-ALD) process. The morphological, structural, physical, chemical, and electrochemical properties of the IrO2 thin films are reported. To the best of our knowledge, the electrochemical characterization of the electrode/electrolyte interface in terms of charge injection capacity, charge storage capacity, and double-layer capacitance for IrO2 grown by PA-ALD was not reported yet. IrO2 grown on PtSi reveals a double-layer capacitance (Cdl) above 300 µF∙cm−2, and a charge injection capacity of 0.22 ± 0.01 mC∙cm−2 for an electrode of 1.0 cm2, confirming IrO2 grown by PA-ALD as an excellent material for neuroelectronic applications.

show abstract

Nanowire based bioprobes for electrical monitoring of electrogenic cells

Cited by 10 publications

References 33 publications

Borna disease virus docks on neuronal DNA double-strand breaks to replicate and dampens neuronal activity

Borna disease virus docks on neuronal DNA double-strand breaks to replicate and dampens neuronal activity

Lab-on-Chip Microsystems for Ex Vivo Network of Neurons Studies: A Review

Plasma-Assisted Atomic Layer Deposition of IrO2 for Neuroelectronics

Contact Info

Product

Resources

About