Nanotribological Perspectives in Tissue Engineering

D’Acunto, Mario; Ciardelli, Gianluca; Rechichi, A.; Montevecchi, Franco Maria; Giusti, P.

doi:10.1007/978-3-540-36807-6_31

Cited by 3 publications

(5 citation statements)

References 93 publications

(110 reference statements)

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“…In addition, the obtained results suggested that the formation of crosslinks increased the local elastic modulus as compared to uncrosslinked areas, resulting in a gradient of the surface elastic modulus. Cells interact with the substrate by means of their focal adhesion points, which positioning depends on the surface topography and the surface elastic characteristics …”

Section: Discussionmentioning

confidence: 99%

Silk Fibroin/Gelatin Blend Films Crosslinked with Enzymes for Biomedical Applications

Taddei

Chiono

Anghileri

et al. 2013

Macromolecular Bioscience

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Microbial transglutaminase (mTG) and mushroom tyrosinase (MT) are used to crosslink B. mori silk fibroin/gelatin (SF/G) films. Crosslinked and uncrosslinked SF/G films show no phase separation. The thermal behavior and the conformational structure of SF/G films are strongly affected by blending and enzymatic treatment. Formation of high thermally stable crosslinked macromolecular species is observed, suggesting the occurrence of strong intermolecular interactions between the two polymers as confirmed by FT-Raman spectroscopy. Preliminary in vitro tests show that MT-crosslinked blends with G amounts ≥40% and mTG-crosslinked SF/G 60/40 films support C2C12 cardiomyocyte adhesion and proliferation.

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Section: Discussionmentioning

confidence: 99%

Silk Fibroin/Gelatin Blend Films Crosslinked with Enzymes for Biomedical Applications

Taddei

Chiono

Anghileri

et al. 2013

Macromolecular Bioscience

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“…Conversely, hMSCs to be chondro‐induced were prepared using the ‘pellet’ method 24. Briefly, this method consisted of gently centrifuging a hMSC suspension that contained 2.5 × 10 5 cells (at 400 g for 6 min) using a 15 mL falcon to obtain a cell pellet.…”

Section: Experimental Partmentioning

confidence: 99%

“…If we limit ourselves to surface mechanical characterization of cells, which has recently become of great interest, the fundamental AFM modes to be considered are: contact mode, tapping mode, force modulation mode, and phase imaging 24. When working in contact mode, the tip is in permanent contact with the sample surface.…”

Section: Experimental Partmentioning

confidence: 99%

“…The unique resolution capability of most AFM techniques is based on the ultra‐sharp AFM probe (tips with a radii of typically 40–60 nm) attached to a flexible cantilever and accurate ceramic piezoelements, which allow the sample to be scanned in the xy plane. AFM has recently found numerous applications in the biological sciences, because of its ability to explore sample surfaces at the micro/nanoscale level under a variety of environmental conditions to simultaneously investigate different chemical or physical features of the same surface 24. In connection with AFM, force modulation microscopy (FMM) can be used to detect variations in the mechanical properties of surface cells, such as surface compliance of hardness of different cytoskeleton components.…”

Section: Introductionmentioning

confidence: 99%

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A Micro/Nanoscale Surface Mechanical Study on Morpho‐Functional Changes in Multilineage‐Differentiated Human Mesenchymal Stem Cells

Danti

D’Acunto

Trombi

et al. 2007

Macromolecular Bioscience

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In recent years MSCs have become a very attractive tool in tissue engineering and regenerative medicine because of their ability to be committed along several lineages through chemical or physical stimuli. Nevertheless their therapeutic potential and plasticity are not yet totally understood. This report describes the use of AFM together with conventional microscopies to obtain mechanical information on cell surfaces and deposited extra cellular matrix molecules, after inducing the differentiation of human MSCs towards three typical mesoderm phenotypes. The aim is to correlate morphological, functional, and mechanical aspects of human MSCs to obtain a deeper understanding of their great potential.

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“…To overcome these problems, covalent functionalization of AFM tip may be performed. Interaction forces of tip-bound ligands and surface-immobilized receptors are measured in force-distance cycles (Force Spectroscopy) [167]. Force measurements by AFM allow the analysis of molecular interactions in terms of kinetic rate constants, structural parameters of binding pockets, molecular dynamics of the recognition process, and the energy range of interactions [168].…”

Section: Dynamic-mode Afm For the Characterization Of Organosilane Sementioning

confidence: 99%

Biomimetic Tailoring of the Surface Properties of Polymers at the Nanoscale: Medical Applications

Chiono

Descrovi

Sartori

et al. 2010

Scanning Probe Microscopy in Nanoscience and Nanotechnology 2

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New-generation biomaterials are information-rich and incorporate biologically active components derived from Nature. The chapter reviews current approaches for the realization of biomimetic coatings for tissue engineering applications, via functionalization with bioactive molecules such as native long chains of extracellular matrix (ECM) proteins as well as short peptide sequences derived from intact ECM proteins. Biomimetic materials provide cues for cell-matrix interactions, favouring cell adhesion, proliferation, spreading, and differentiation. The recently developed scanning probe techniques for optical and spectroscopic characterization of surfaces are also described, providing advanced topographical imaging and sensitive force measurements. The spectroscopic imaging of surfaces at the nanoscale provides insight into the function and structure of biomimetic molecules and represents a tool for tailoring the surface design.

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Nanotribological Perspectives in Tissue Engineering

Cited by 3 publications

References 93 publications

Silk Fibroin/Gelatin Blend Films Crosslinked with Enzymes for Biomedical Applications

Silk Fibroin/Gelatin Blend Films Crosslinked with Enzymes for Biomedical Applications

A Micro/Nanoscale Surface Mechanical Study on Morpho‐Functional Changes in Multilineage‐Differentiated Human Mesenchymal Stem Cells

Biomimetic Tailoring of the Surface Properties of Polymers at the Nanoscale: Medical Applications

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