Nanotransfer printing of plasmonic nano-pleat arrays with ultra-reduced nanocavity width using perfluoropolyether molds

Liang, Chia Ching; Chang, Wen Huei; Lin, Chun Hung

doi:10.1039/c6tc00064a

Cited by 4 publications

(5 citation statements)

References 81 publications

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“…The dish made of polystyrene with oxygen plasma treatment on the surface is the most widely used culture platform . The integration of an LSPR layer with the dishes could be possible using the nanotransfer printing technique. , Assuming that the total area of the sampling region is 25 mm 2 on a 100 mm culture dish, there is only 0.45% of the cells that grow on the GNDA when the cells reach confluency. We believe that the small ratio of cells on the GNDAs would not influence the gene expression of the entire culture system.…”

Section: Discussionmentioning

confidence: 99%

Quantifying Cell Confluency by Plasmonic Nanodot Arrays to Achieve Cultivating Consistency

et al. 2019

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The determination of cell confluency and subculture timing for cell culture consistency is crucial in the field of cell-based research, but there is no universal standard concerning optimal confluence. In this study, gold nanodot arrays on glass substrates were used as culture substrates, and their spectral shifts of localized surface plasmon resonance (LSPR) were employed to monitor cell growth and quantify cell confluency. Experiments including cell counting, metabolic activity, focal adhesion, and cell cycle were also performed to confirm the cell growth monitoring accuracy of the LSPR signals. The LSPR signal exhibited the same trends like the increase of cell numbers and cell metabolic activity and reached the maximum as the cell growth achieved confluency, suggesting its great capability as an effective indicator to predict suitable subculture timing. The proposed sensing approach is a noninterventional, nondestructive, real-time, and useful tool to help biologists quantify the optimal subculture timing, achieve cell culture consistency, and obtain reproducible experimental results efficiently.

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Section: Discussionmentioning

confidence: 99%

Quantifying Cell Confluency by Plasmonic Nanodot Arrays to Achieve Cultivating Consistency

et al. 2019

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“…11,14 These sensitivities comparable to the state-of-the-art RI SPR sensors with more complicated nanostructures are listed in Table 1. [41][42][43][44][45][46][47][48] Some conclusions can be obtained from this work. First, thanks to the plasmon hybridization effect of the LRSPR configuration, LRSPR modes were generated with higher RI sensitivity compare with traditional SPR modes, indicating that the ring structure, refractive indices-symmetrical spacer layers and Au mirror were the main factors for improving RI sensitivity.…”

Section: Influence Of the Geometry Shape Of Plasmonic Structures To R...mentioning

confidence: 67%

“…11,14 These sensitivities comparable to the state-of-the-art RI SPR sensors with more complicated nanostructures are listed in Table 1. 41–48…”

Section: Resultsmentioning

confidence: 99%

High-sensitivity long-range surface plasmon resonance sensing assisted by gold nanoring cavity arrays and nanocavity coupling

Luo

Tan

et al. 2023

Phys. Chem. Chem. Phys.

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“…The AuNM chip was fabricated through nTP. , A flexible PFPE working mold patterned with a nanodisk array was first replicated from a silicon master mold and then deposited with a 50 nm gold film through electron-beam evaporation. The gold-deposited PFPE mold was attached to a flat PC substrate and placed in our home-built imprint chamber .…”

Section: Methodsmentioning

confidence: 99%

“…In this study, we developed a simple process for fabricating gold nanomushroom (AuNM) arrays on polycarbonate (PC) sheets based on the nanotransfer printing (nTP) technique. , PC is lightweight, flexible, and optically transparent, which is commonly employed as substrates for portable applications. The nTP process involves only gold deposition on the polymer mold and pattern transfer to a PC sheet, which is simple and efficient.…”

Section: Introductionmentioning

confidence: 99%

Noninvasive Detection of Bladder Cancer Markers Based on Gold Nanomushrooms and Sandwich Immunoassays

Yang

Chang

Chiu

et al. 2023

ACS Appl. Nano Mater.

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Bladder cancer is one of the most common malignancies in the urinary system. Cystoscopy is the traditional standard diagnostic method for bladder cancer with subsequent biopsy or surgery. However, this method is uncomfortable for most patients because it requires anesthesia and possibly causes infections. Because of the high recurrence rate of bladder cancer, a rapid, low-cost, high-sensitivity, and noninvasive sensing method is needed. This study employed gold nanomushroom (AuNM) chips for bladder cancer biomarker detection, combining the benefits of sandwich immunoassay and localized surface plasmon resonance (LSPR) sensing. With a metal nanotransfer printing technique, which is cheap and straightforward, the AuNMs were patterned on flexible polycarbonate (PC) sheets. The gold caps stood above PC stems and provided ample spatial areas for capturing the biomarkers to be sensed. Three biomarkers served as the antigens and analytes, including human complement factor H (CFH), hyaluronic acid (HA), and nuclear matrix protein 22 (NMP22). Different antibodies, against the same biomarker, were covalently conjugated to AuNMs or gold nanoparticles, respectively. When the antibody–antigen–antibody sandwich structure formed, the plasmonic coupling between the AuNM surface and the gold nanoparticles significantly enhanced LSPR signals. The LSPR red shifts correlated quantitatively with the concentrations of the biomarkers. The limits of detection were 6.5, 8.3, and 7.0 pg/mL for CFH, HA, and NMP22, respectively. The chip’s specificity was tested and confirmed, excluding the nonspecific binding and false-positive possibility. The sensing performance of this sandwich immunoassay-based AuNM chip was better than that of the commercialized enzyme-linked immunosorbent assay. It provided a rapid, label-free, and easy operating platform for diagnosing and monitoring bladder cancer.

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Nanotransfer printing of plasmonic nano-pleat arrays with ultra-reduced nanocavity width using perfluoropolyether molds

Abstract: Ultra-reduced nanocavities in plasmonic nano-pleat arrays achieved by gold sputtering on perfluoropolyether molds and one-step nanotransfer printing.

Cited by 4 publications

References 81 publications

Quantifying Cell Confluency by Plasmonic Nanodot Arrays to Achieve Cultivating Consistency

Quantifying Cell Confluency by Plasmonic Nanodot Arrays to Achieve Cultivating Consistency

High-sensitivity long-range surface plasmon resonance sensing assisted by gold nanoring cavity arrays and nanocavity coupling

Noninvasive Detection of Bladder Cancer Markers Based on Gold Nanomushrooms and Sandwich Immunoassays

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