Nanotherapeutics using all-natural materials. Effective treatment of wound biofilm infections using crosslinked nanoemulsions

Li, Cheng Hsuan; Landis, Ryan F.; Makabenta, Jessa Marie; Nabawy, Ahmed; Tronchet, Tiphaine; Archambault, Danielle; Liu, Yuanchang; Huang, Rui; Golan, Morgane; Cui, Wei; Mager, Jesse; Gupta, Akash; Schmidt-Malan, Suzannah M.; Patel, Robin; Rotello, Vincent M.

doi:10.1039/d0mh01826k

Cited by 31 publications

(30 citation statements)

References 37 publications

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“…In this study, oxanorbornene-based PNPs mimic antimicrobial peptides, but synthetic approaches enable fabrication of small size of nanoparticles, exhibiting multivalent interactions with the bacteria, as well as traverse the biofilm EPS matrix. Nanoparticles access a variety of antibacterial mechanisms, making the barrier to resistance development higher than for small molecule antibiotics that target specific processes [ 14 , 15 , 16 ]. The antimicrobial PONI-based polymers employed in this work are functionalized synthetic polymers that consist of cationic trimethylamine (TMA) head groups bridged to the PONI backbone by hydrophobic alkyl chains.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, there is an urgent need for new treatment regimens to address multispecies biofilm infections. Recent advances in nanomaterial-based therapeutics provide a promising opportunity to effectively address difficult-to-treat bacterial and biofilm infections [14][15][16][17]. Polymeric nanoparticles provide versatile additions to the antimicrobial arsenal [18,19].…”

Section: Introductionmentioning

confidence: 99%

“…Recent advances in nanomaterial-based therapeutics provide a promising opportunity to effectively address difficult-to-treat bacterial and biofilm infections [ 14 , 15 , 16 , 17 ]. Polymeric nanoparticles provide versatile additions to the antimicrobial arsenal [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Polymeric Nanoparticles Active against Dual-Species Bacterial Biofilms

Makabenta

Park

et al. 2021

Molecules

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Biofilm infections are a global public health threat, necessitating new treatment strategies. Biofilm formation also contributes to the development and spread of multidrug-resistant (MDR) bacterial strains. Biofilm-associated chronic infections typically involve colonization by more than one bacterial species. The co-existence of multiple species of bacteria in biofilms exacerbates therapeutic challenges and can render traditional antibiotics ineffective. Polymeric nanoparticles offer alternative antimicrobial approaches to antibiotics, owing to their tunable physico-chemical properties. Here, we report the efficacy of poly(oxanorborneneimide) (PONI)-based antimicrobial polymeric nanoparticles (PNPs) against multi-species bacterial biofilms. PNPs showed good dual-species biofilm penetration profiles as confirmed by confocal laser scanning microscopy. Broad-spectrum antimicrobial activity was observed, with reduction in both bacterial viability and overall biofilm mass. Further, PNPs displayed minimal fibroblast toxicity and high antimicrobial activity in an in vitro co-culture model comprising fibroblast cells and dual-species biofilms of Escherichia coli and Pseudomonas aeruginosa. This study highlights a potential clinical application of the presented polymeric platform.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polymeric Nanoparticles Active against Dual-Species Bacterial Biofilms

Makabenta

Park

et al. 2021

Molecules

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“…Based on a murine wound biofilm model, it was found that nanoemulsions could reduce bacterial loads in wounds and accelerate wound healing. In a recent study, Li et al [183] . reported on a bacterial biofilm in vivo treatment that accelerated wound healing through the use of all‐natural materials.…”

Section: Wound Biofilm Therapymentioning

confidence: 99%

“…In ar ecent study,L ie tal. [183] reported on ab acterial biofilm in vivo treatment that accelerated wound healing through the use of all-natural materials.G elatin was stabilized by photo-crosslinking with riboflavin (vitamin B2) and carvacrol (the primary component in oregano oil) as the active antimicrobial component (Figure 19 a). Thee ngineered nanoemulsions were demonstrated to have broad-spectrum antimicrobial activity against…”

Section: Nanoemulsionsmentioning

confidence: 99%

Advances in the Sensing and Treatment of Wound Biofilms

et al. 2022

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Wound biofilms represent a particularly challenging problem in modern medicine. They are increasingly antibiotic resistant and can prevent the healing of chronic wounds. However, current treatment and diagnostic options are hampered by the complexity of the biofilm environment. In this review, we present new chemical avenues in biofilm sensors and new materials to treat wound biofilms, offering promise for better detection, chemical specificity, and biocompatibility. We briefly discuss existing methods for biofilm detection and focus on novel, sensor‐based approaches that show promise for early, accurate detection of biofilm formation on wound sites and that can be translated to point‐of‐care settings. We then discuss technologies inspired by new materials for efficient biofilm eradication. We focus on ultrasound‐induced microbubbles and nanomaterials that can both penetrate the biofilm and simultaneously carry active antimicrobials and discuss the benefits of those approaches in comparison to conventional methods.

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A Photo‐Therapeutic Nanocomposite with Bio‐Responsive Oxygen Self‐Supplying Combats Biofilm Infections and Inflammation from Drug‐Resistant Bacteria

Yuan

Xiao

et al. 2023

Adv Funct Materials

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The use of non‐antibiotic strategies to combat refractory drug‐resistant bacterial infections, especially biofilms and accompanying inflammation, has recently aroused widespread interest. Herein, a photo‐therapeutic nanocomposite with bio‐responsive oxygen (O2) self‐supplying is introduced by integrating manganese dioxide (MnO2) nanozymes onto photosensitizer (indocyanine green, ICG)‐loaded mesoporous polydopamine nanoparticles (MPDA), namely MI‐MPDA NPs. MI‐MPDA can activate O2 generation in the infection microenvironment, thereby effectively alleviating biofilm hypoxia. Under near‐infrared light (NIR) irradiation, continuous O2 supplying further boosts the level of singlet oxygen (1O2), enabling robust biofilm elimination through O2‐potentiated photodynamic/photothermal therapy. Interestingly, MI‐MPDA down‐regulates the factor expression of inflammatory signaling pathways through MnO2‐mediated reactive oxygen species scavenging, which ameliorates the inflammatory condition. Meanwhile, O2 supplying prevents the M1‐phenotype switch of macrophages from the overexpression of hypoxia‐inducible factor‐1α (HIF‐1α), thereby prompting macrophage reprogramming toward pro‐regenerative M2‐phenotype. In the mouse models of subcutaneous implant‐associated infection caused by methicillin‐resistant Staphylococcus aureus (MRSA) biofilms and burn infection caused by Pseudomonas aeruginosa biofilms, NIR‐irradiated MI‐MPDA not only effectively eliminates the formed biofilms, but also alleviates the oxidative stress and accompanying inflammation, and drives the cascade reaction of immunomodulation‐wound healing. Overall, this O2‐potentiated photo‐therapeutic strategy provides a reliable tool for combating biofilm infections and inflammation from drug‐resistant bacteria.

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Nanotherapeutics using all-natural materials. Effective treatment of wound biofilm infections using crosslinked nanoemulsions

Abstract: Bacterial wound infections are a threat to public health. Although antibiotics currently provide front-line treatments for bacterial infections, the development of drug resistance coupled with the defenses provided through biofilm...

Cited by 31 publications

References 37 publications

Polymeric Nanoparticles Active against Dual-Species Bacterial Biofilms

Polymeric Nanoparticles Active against Dual-Species Bacterial Biofilms

Advances in the Sensing and Treatment of Wound Biofilms

A Photo‐Therapeutic Nanocomposite with Bio‐Responsive Oxygen Self‐Supplying Combats Biofilm Infections and Inflammation from Drug‐Resistant Bacteria

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