Nanotechnology for photodynamic therapy: a perspective from the Laboratory of Dr. Michael R. Hamblin in the Wellman Center for Photomedicine at Massachusetts General Hospital and Harvard Medical School

Hamblin, Michael R.; Chiang, Long Y.; Lakshmanan, Shanmugamurthy; Huang, Ying‐Ying; García-Díaz, María; Karimi, Mahdi; Rastelli, Alessandra Nara de Souza; Chandran, Rakkiyappan

doi:10.1515/ntrev-2015-0027

Cited by 44 publications

(22 citation statements)

References 92 publications

(91 reference statements)

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“…In the presence of ambient molecular oxygen, the triplet Ag-NPs can react in photochemical reactions involving hydrogen or electron transfer reactions between the excited state PS and other molecules in the environment (usually oxygen). These electron transfer reactions produce (directly or indirectly) ROS that are harmful to cells, such as superoxide (O 2 ⋅-), hydrogen peroxide (H 2 O 2 ), hydroxyl radicals (HO ⋅ ), and hydroperoxyl radicals (HOO ⋅ ) [32,35].…”

Section: Discussionmentioning

confidence: 99%

Enhancement of the Antibacterial Efficiency of Silver Nanoparticles against Gram-Positive and Gram-Negative Bacteria Using Blue Laser Light

Al‐Sharqi¹,

Apun²,

Vincent³

et al. 2019

International Journal of Photoenergy

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Silver nanoparticles (Ag-NPs) possess excellent antibacterial properties and are considered to be an alternative material for treating antibiotic-resistant bacteria. The present study was aimed at enhancing the antibacterial efficiency of Ag-NPs using visible laser light against Escherichia coli and Staphylococcus aureus in vitro. Four concentrations of Ag-NPs (12.5, 25, 50, and 100 μg/ml), synthesized by the chemical reduction method, were utilized to conduct the antibacterial activity of prepared Ag-NPs. The antibacterial efficiencies of photoactivated Ag-NPs against both bacteria were determined by survival assay after exposure to laser irradiation. The mechanism of interactions between Ag-NPs and the bacterial cell membranes was then evaluated via scanning electron microscopy (SEM) and reactive oxygen species analysis to study the cytotoxic action of photoactivated Ag-NPs against both bacterial species. Results showed that the laser-activated Ag-NP treatment reduced the surviving population to 14% of the control in the E. coli population, while the survival in the S. aureus population was reduced to 28% of the control upon 10 min exposure time at the concentration of 50 μg/ml. However, S. aureus showed lower sensitivity after photoactivation compared to E. coli. Moreover, the effects depended on the concentration of Ag-NPs and exposure time to laser light. SEM images of treated bacterial cells indicated that substantial morphological changes occurred in cell membranes after treatment. The results suggested that Ag-NPs in the presence of visible light exhibit strong antibacterial activity which could be used to inactivate harmful and pathogenic microorganisms.

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Section: Discussionmentioning

confidence: 99%

Enhancement of the Antibacterial Efficiency of Silver Nanoparticles against Gram-Positive and Gram-Negative Bacteria Using Blue Laser Light

Al‐Sharqi¹,

Apun²,

Vincent³

et al. 2019

International Journal of Photoenergy

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“…This group has emphasized that these new strategies can play an important role in the solubility of the PSs, metal nanoparticles can carry out plasmon resonance enhancement, and fullerenes can act as PSs, themselves. Regarding its application in the field of dentistry, it is expected that these nanomaterials will play an important role in restorative dentistry [59]. …”

Section: Application Of Pdt In Dentistrymentioning

confidence: 99%

The application of antimicrobial photodynamic therapy (aPDT) in dentistry: a critical review

et al. 2016

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In recent years there have been an increasing number of in vitro and in vivo studies that show positive results regarding antimicrobial photodynamic therapy (aPDT) used in dentistry. These include applications in periodontics, endodontics, and mucosal infections caused by bacteria present as biofilms. Antimicrobial photodynamic therapy is a therapy based on the combination of a non-toxic photosensitizer (PS) and appropriate wavelength visible light, which in the presence of oxygen is activated to produce reactive oxygen species (ROS). ROS induce a series of photochemical and biological events that cause irreversible damage leading to the death of microorganisms. Many light-absorbing dyes have been mentioned as potential PS for aPDT and different wavelengths have been tested. However, there is no consensus on a standard protocol yet. Thus, the goal of this review was to summarize the results of research on aPDT in dentistry using the PubMed database focusing on recent studies of the effectiveness aPDT in decreasing microorganisms and microbial biofilms, and also to describe aPDT effects, mechanisms of action and applications.

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“…superoxide radicals, free hydroxyl radicals and hydrogen peroxide [317, 318]. PDT can be used to kill various microorganisms (bacteria, viruses, yeasts, fungi, parasites and cancer cells) [319, 320]. PDT has been reported to treat dermatological diseases and infections [321].…”

Section: Nanotechnology For Treatment Of Burn Infections and Woundmentioning

confidence: 99%

Nanomedicine and advanced technologies for burns: Preventing infection and facilitating wound healing

Jahromi

Zangabad

Basri

et al. 2018

Advanced Drug Delivery Reviews

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375

189

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According to the latest report from the World Health Organization, an estimated 265,000 deaths still occur every year as a direct result of burn injuries. A widespread range of these deaths induced by burn wound happens in low- and middle-income countries, where survivors face a lifetime of morbidity. Most of the deaths occur due to infections when a high percentage of the external regions of the body area is affected. Microbial nutrient availability, skin barrier disruption, and vascular supply destruction in burn injuries as well as systemic immunosuppression are important parameters that cause burns to be susceptible to infections. Topical antimicrobials and dressings are generally employed to inhibit burn infections followed by a burn wound therapy, because systemic antibiotics have problems in reaching the infected site, coupled with increasing microbial drug resistance. Nanotechnology has provided a range of molecular designed nanostructures (NS) that can be used in both therapeutic and diagnostic applications in burns. These NSs can be divided into organic and non-organic (such as polymeric nanoparticles (NPs) and silver NPs, respectively), and many have been designed to display multifunctional activity. The present review covers the physiology of skin, burn classification, burn wound pathogenesis, animal models of burn wound infection, and various topical therapeutic approaches designed to combat infection and stimulate healing. These include biological based approaches (e.g. immune-based antimicrobial molecules, therapeutic microorganisms, antimicrobial agents, etc.), antimicrobial photo- and ultrasound-therapy, as well as nanotechnology-based wound healing approaches as a revolutionizing area. Thus, we focus on organic and non-organic NSs designed to deliver growth factors to burned skin, and scaffolds, dressings, etc. for exogenous stem cells to aid skin regeneration. Eventually, recent breakthroughs and technologies with substantial potentials in tissue regeneration and skin wound therapy (that are as the basis of burn wound therapies) are briefly taken into consideration including 3D-printing, cell-imprinted substrates, nano-architectured surfaces, and novel gene-editing tools such as CRISPR-Cas.

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Nanotechnology for photodynamic therapy: a perspective from the Laboratory of Dr. Michael R. Hamblin in the Wellman Center for Photomedicine at Massachusetts General Hospital and Harvard Medical School

Cited by 44 publications

References 92 publications

Enhancement of the Antibacterial Efficiency of Silver Nanoparticles against Gram-Positive and Gram-Negative Bacteria Using Blue Laser Light

Enhancement of the Antibacterial Efficiency of Silver Nanoparticles against Gram-Positive and Gram-Negative Bacteria Using Blue Laser Light

The application of antimicrobial photodynamic therapy (aPDT) in dentistry: a critical review

Nanomedicine and advanced technologies for burns: Preventing infection and facilitating wound healing

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