Nanotechnology for Enrichment and Detection of Circulating Tumor Cells

Bhana, Saheel; Wang, Yongmei; Huang, Xiaohua

doi:10.2217/nnm.15.32

Cited by 71 publications

(69 citation statements)

References 136 publications

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“…Thus, a combination of both EpCAM- and non-EpCAM-based CTC technologies seems to be needed in order to better assess the presence of CTCs in patients with SCLC and to evaluate their biological and clinical relevance. It should be noted that new and promising methodologies have also been developed for the detection of CTCs based on nanoparticles575859606162; however, these methods have serious limitations, such as the higher cost and the requirement of special equipment compared to the simple immunofluorescence staining.…”

Section: Discussionmentioning

confidence: 99%

TTF-1- and/or CD56-positive Circulating Tumor Cells in patients with small cell lung cancer (SCLC)

Messaritakis

Stoltidis

Κotsakis

et al. 2017

Sci Rep

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The aim of the study was to evaluate the phenotypic CTCs heterogeneity (TTF-1+ and/or CD56+) in SCLC patients and correlate it with the CellSearch. Peripheral blood was obtained from 108 consecutive patients. CTCs were detected by CellSearch and double-immunofluorescence using anti-CD45, anti-TTF-1 and anti-CD56 antibodies. Before chemotherapy TTF-1+/CD45−, CD56+/CD45− and TTF-1+/CD56+ CTCs were detected in 66(61.1%), 55(50.9%) and 46(42.6%) patients, respectively; 60.2% of patients were CellSearch+. Among the 22 patients with 0 CTCs/7.5 ml on CellSearch, TTF-1+/CD45−, CD56+/CD45− and TTF-1+/CD56+ CTCs were detected in 8(36.4%), 6(27.3) and 6(27.3%) patients, respectively; no CK+/EpCAM+ or TTF1+/EpCAM+ CTCs were detected in these patients. One-chemotherapy cycle decreased both the number of positive patients (p < 0.001) and their CTC number (p < 0.001), irrespectively of their phenotype and the detection method. The incidence and number of the different CTC subpopulations on PD, was significantly increased at their baseline levels. Multivariate analysis revealed that the increased number of CTCs at baseline and on PD were significantly associated with decreased PFS (p = 0.048) and OS (p = 0.041), respectively. There is an important CTC heterogeneity in such patients according to the expression of TTF-1 and CD56 which could detect EpCAM− CTC subpopulations and, thus, undetectable by CellSearch. These CTC subpopulations are dynamically correlated with treatment efficacy and disease-progression.

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Section: Discussionmentioning

confidence: 99%

TTF-1- and/or CD56-positive Circulating Tumor Cells in patients with small cell lung cancer (SCLC)

Messaritakis

Stoltidis

Κotsakis

et al. 2017

Sci Rep

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“…Their unique superparamagnetic properties offer the possibility to manipulate them under an external magnetic field: their huge magnetic moment causes them to move rapidly in the applied field and as they show no remanence, they fully disperse once the field is removed. Therefore, they are widely used in several kinds of biological applications such as bioseparation, cell sorting, biosensing,, drug delivery, hyperthermia therapy and cell imaging . The implementation of surface modification protocols is fundamental to provide functional properties to these NPs.…”

Section: Introductionmentioning

confidence: 99%

“…The implementation of surface modification protocols is fundamental to provide functional properties to these NPs. Magnetic NPs can be functionalized with different kinds of biomolecules such as antibodies, oligonucleotides, carbohydrates or enzymes, depending on the targeted application. However, other ligands with additional physico‐chemical properties such as redox probes, fluorescent dyes,, gold colloids, or quantum dots, can also be immobilized onto magnetic NPs.…”

Section: Introductionmentioning

confidence: 99%

Polyamidoamine Dendrimers as Crosslinkers for Efficient Electron Transfer between Redox Probes onto Magnetic Nanoparticles

et al. 2018

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The efficient conjugation of ferrocenecarboxylic acid (Fc), a usual electroactive probe, onto COOH‐modified magnetic nanoparticles (NPs) is reported. Amine crosslinkers were used to couple COOH groups from NPs and from Fc. The coupling efficiencies using different generations of polyamidoamine (PAMAM) dendrimer and ethylenediamine (EDA) were compared through zeta potential measurements, square‐wave voltammetry (SWV) and NH2 titration. Highest PAMAM generations led to NH2‐modified NPs with increased zeta potential and NH2 density compared to lowest ones. Moreover, no aggregation was observed after crosslinker grafting. SWV was applied to characterize Fc‐modified NPs. PAMAM generations 0–3 improved the electrochemical signal in comparison to EDA. However, increasing PAMAM generations led to NPs aggregation. Quantitative results supported the fact that PAMAM generation 0 was the best compromise for large electrochemical signal without NPs aggregation.

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“…In the medical and pharmaceutical fields, it is applied for detecting tumor cells, delivering nanomedicines through the skin or gastrointestinal tract, enhancing radiation therapy, and extending the pharmacokinetic properties of drugs [2,3,15,20]. In the food industry, nanotechnology is used in agriculture, food processing and packaging, and supplements [13,18].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Extraction of Bioactive Compounds from Bee Pollen by Wet-grinding Technology

Choi¹,

Suh²,

Chung³

2016

Journal of Life Science

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Bee pollen is produced by honeybees and is considered one of the most balanced and nourishing nutritional supplements available. Historically, bee pollen has been prescribed for its healing properties and consumed for its high-energy supply. Recent research has provided evidence that bee pollen has diverse biological activities, such as anti-oxidant, anti-inflammatory, anti-bacterial, and even anti-cancer effects. However, the outer membrane of the pollen grain, exine, is highly resistant to most acidic solutions, high pressure, and even digestive enzymes, and the resulting low bioavailability limits its nutritional and clinical applications. This study applied a wet-grinding method to destroy the exine effectively, and it then examined the pollen's enhanced biological activity. First, microscopic observations provided strong evidence that wet grinding destroyed the exine time-dependently. In addition, the content of polyphenols, well-known ingredients of bee pollen and used as internal standards for the quality control of commercial pollen preparations, increased up to 11-fold with wet grinding. Further, the anti-oxidant activity demonstrated on the ABTS anti-oxidant assay, as well as the DPPH radical scavenging assay, was also dramatically increased. Together, the results presented here support a new technology by which bee pollen can be used as a resource for medical, nutritional, and cosmetic applications.

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Nanotechnology for Enrichment and Detection of Circulating Tumor Cells

Cited by 71 publications

References 136 publications

TTF-1- and/or CD56-positive Circulating Tumor Cells in patients with small cell lung cancer (SCLC)

TTF-1- and/or CD56-positive Circulating Tumor Cells in patients with small cell lung cancer (SCLC)

Polyamidoamine Dendrimers as Crosslinkers for Efficient Electron Transfer between Redox Probes onto Magnetic Nanoparticles

Enhanced Extraction of Bioactive Compounds from Bee Pollen by Wet-grinding Technology

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