Nanotechnology Based Repositioning of an Anti-Viral Drug for Non-Small Cell Lung Cancer (NSCLC)

Parvathaneni, Vineela; Goyal, Mimansa; Kulkarni, Nishant S.; Shukla, Snehal K.; Gupta, Vivek

doi:10.1007/s11095-020-02848-2

Cited by 16 publications

(16 citation statements)

References 72 publications

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“…The cells were washed with PBS and stained by incubating with crystal violet (0.2%) for 1 h. Colonies containing more than 50 cells were counted using Open CFU software (Version 4.8) and imaged using a digital camera. The % growth of the colonies for each treatment group was calculated relative to the number of colonies observed for the control group. , All data are presented as mean ± SD or SEM ( n = 3–8). Results represent the average of three independent trials ( n = 6 for each trial).…”

Section: Methodsmentioning

confidence: 99%

“…Optical imaging was performed using an LMI-6000 inverted microscope (LAXCO, Bothell, WA, USA), and transmitted light microscope images were collected using SEBA view software. Changes in the morphology of spheroids, such as spheroid diameter and spheroid volume, were calculated using ImageJ software version 2.2.0. , All data are presented as mean ± SD or SEM ( n = 3–8). Results represent the average of three independent trials ( n = 6 for each trial).…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, media were replaced by 100 μL of kit reagent, followed by incubation in the dark for 30 min at room temperature. The labeled spheroid cells were imaged at 4× using a fluorescence microscope EVOS FL cell imaging system (Thermo Fisher Scientific, Waltham, MA, USA) . All data are presented as mean ± SD or SEM ( n = 3–8).…”

Section: Methodsmentioning

confidence: 99%

“…The % growth of the colonies for each treatment group was calculated relative to the number of colonies observed for the control group. 47,48 All data are presented as mean ± SD or SEM (n = 3−8). Results represent the average of three independent trials (n = 6 for each trial).…”

Section: ■ Conclusionmentioning

confidence: 99%

“…Changes in the morphology of spheroids, such as spheroid diameter and spheroid volume, were calculated using ImageJ software version 2.2.0. 47,48 All data are presented as mean ± SD or SEM (n = 3−8). Results represent the average of three independent trials (n = 6 for each trial).…”

Section: ■ Conclusionmentioning

confidence: 99%

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Small-Molecule Gankyrin Inhibition as a Therapeutic Strategy for Breast and Lung Cancer

et al. 2022

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Gankyrin is an oncoprotein responsible for the development of numerous cancer types. It regulates the expression levels of multiple tumor suppressor proteins (TSPs) in liver cancer; however, gankyrin’s regulation of these TSPs in breast and lung cancers has not been thoroughly investigated. Additionally, no small-molecule gankyrin inhibitor has been developed which demonstrates potent anti-proliferative activity against gankyrin overexpressing breast and lung cancers. Herein, we are reporting the structure-based design of gankyrin-binding small molecules which potently inhibited the proliferation of gankyrin overexpressing A549 and MDA-MB-231 cancer cells, reduced colony formation, and inhibited the growth of 3D spheroids in an in vitro tumor simulation model. Investigations demonstrated that gankyrin inhibition occurs through either stabilization or destabilization of its 3D structure. These studies shed light on the mechanism of small-molecule inhibition of gankyrin and demonstrate that gankyrin is a viable therapeutic target for the treatment of breast and lung cancer.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: ■ Conclusionmentioning

confidence: 99%

Section: ■ Conclusionmentioning

confidence: 99%

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