Nanotechnology-based manipulation of dendritic cells for enhanced immunotherapy strategies

Klippstein, Rebecca; Pozo, David

doi:10.1016/j.nano.2010.01.001

Cited by 138 publications

(88 citation statements)

References 32 publications

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“…Particulate formulations enhance the targeting and delivery of both prophyl actic vaccines and therapeutic drugs [7][8][9][10]. With vaccines, the delivery must consider both the antigenic component and the adjuvant formulation to ensure efficacious immune defense induction.…”

Section: Nanoparticle-based Deliverymentioning

confidence: 99%

Functional RNA Delivery Targeted to Dendritic Cells By Synthetic Nanoparticles

McCullough¹,

Bassi²,

Démoulins³

et al. 2012

Therapeutic Delivery

102

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ReviewTargeting the immune system Dendritic cells (DCs) play crucial roles in promoting and regulating immune defenses, providing important targets for prophylactic and therapeutic approaches (FiguRe 1). Particulate formulations, including liposomes and other nanoparticles, have been employed as delivery vehicles. Despite the large number of reports, current in-depth knowledge on the cellular processes involved remains relatively limited, particularly for nucleic acid delivery. Certain structures in the nucleic acid may also signal the cell in the sense of an adjuvant or immunomodulatory activity. Importantly, the optimized characteristics for delivery of an antigen or therapeutic agent may be distinct from those for nucleic acid delivery, especially RNA species. Moreover, RNA delivery for interference therapy will not necessarily require the same delivery routes as mRNA delivery wherein RNA translation is the main requisite.The efficacy of delivery can be further improved by targeting the appropriate cells of the immune system, an area in which nanoparticle-based delivery platforms have found an important niche [1]. Advances with prophylactic applications can also prove valuable for therapeutic applications and vice versa, as seen with RNA delivery. This review will consider how growing knowledge on delivery mechanisms has found application with nucleic acids, in both prophylaxis and therapy, focusing on interaction with DCs.The initial components of the DC endocytic pathways are critically important in determining how the cell handles the delivered material; thereafter, correct cytosolic delivery is a critical element for a number of desired outcomes, including delivery of nucleic acids. Advances made with protein delivery -in particular, vaccines -are of value to highlight the potential of a particular mode of application or the high risk for nucleic acid integrity. Particularly pertinent is the application of cationic elements in the delivery mechanisms and how application of ligands for cell receptors influence intracellular compartmentalization.It is not the aim of the present review to retrace all the fine details of work contributing to our current knowledge. Accordingly, review articles covering areas that have already received considerable attention will be employed and assimilated to provide a more elaborate picture of the current situation. This will allow a focusing on more recent advances, along with problems and pitfalls therein. While advances on protein and DNA delivery will be presented, these will be used to highlight how our current knowledge can be applied to RNA delivery. There are numerous reviews on protein delivery to DC, wherein many of the procedures employed are not relevant for RNA delivery, which must escape into the cytosol undamaged. With DNA delivery, there is also the question of whether the DNA will activate the DC or reach the nucleus for transcription; this latter area has most often Functional RNA delivery targeted to dendritic cells by synthetic nanoparticles Dendritic cell...

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Section: Nanoparticle-based Deliverymentioning

confidence: 99%

Functional RNA Delivery Targeted to Dendritic Cells By Synthetic Nanoparticles

McCullough¹,

Bassi²,

Démoulins³

et al. 2012

Therapeutic Delivery

102

View full text Add to dashboard Cite

show abstract

“…One of the main potential advantages of using NPs for vaccines is based on their capacity to stabilise vaccine antigens while simultaneously acting as an adjuvant 6,[9][10] . Antigens can be encapsulated or conjugated to the NP surface, with both methods offering distinct advantages.…”

Section: Introductionmentioning

confidence: 99%

Multivalent Glycosylation of Fluorescent Gold Nanoclusters Promotes Increased Human Dendritic Cell Targeting via Multiple Endocytic Pathways

Guével

Perrino

Fernández³

et al. 2015

ACS Appl. Mater. Interfaces

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We report the synthesis and characterisation of gold nanoclusters (Au NCs) stabilised by a mixture of zwitterionic and multivalent mannose ligands. Characterisation of this carbohydrated nanosystem confirms its small size (~2 nm), intense red-NIR fluorescence, relatively high affinity to lectin (ConA), and stability in physiological media. Cell studies performed using human monocyte-derived dendritic cells (DCs) show that Au NC uptake efficiency is greatly enhanced by the presence of surface carbohydrate (> 250% compared to non-carbohydrated Au 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 2 NCs) allowing their detection in cells by fluorescence following incubation with concentrations as low as 1 µg.mL -1 . Investigation using electron microscopy and pharmacological inhibitorsindicates that Au NC uptake is mediated by multiple endocytic pathways involving the engulfment of Au NCs into endosomes and partial transport to lysosomes. Results show that clathrin and F-actin dependent pathways play major roles in Au NC uptake by DCs regardless of whether or not they are coated with carbohydrates. In contrast, a specific C-lectin inhibitor induces a 60% decrease in DC particle uptake only for the carbohydrate-coated Au NCs. This study demonstrates that the combination of ultra-small gold NCs and functionalisation with multivalent mannose ligands results in greatly enhanced human DC targeting, presumably due to increased diffusion and target cell binding, respectively.

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“…Some authors have paid attention to this cell line in order to enhance the production of clinical grade DC-derived exosomes for immunotherapy [33]. Properties of DCs have even been enhanced with different nanoparticles [34]. Exosomes from DCs modified to express indoleamine 2,3-dioxygenase (IDO), a tryptophan-degrading enzyme that is important for immune regulation and tolerance maintenance, have been used in the treatment of rheumatoid arthritis [35].…”

Section: Semi-synthetic Exosomes: Biotechnological Modification Of Namentioning

confidence: 99%

Therapeutic biomaterials based on extracellular vesicles: classification of bio‐engineering and mimetic preparation routes

García‐Manrique

Matos

Gutiérrez

et al. 2018

J of Extracellular Vesicle

141

153

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Extracellular vesicles (EVs) are emerging as novel theranostic tools. Limitations related to clinical uses are leading to a new research area on design and manufacture of artificial EVs. Several strategies have been reported in order to produce artificial EVs, but there has not yet been a clear criterion by which to differentiate these novel biomaterials. In this paper, we suggest for the first time a systematic classification of the terms used to build up the artificial EV landscape, based on the preparation method. This could be useful to guide the derivation to clinical trial routes and to clarify the literature. According to our classification, we have reviewed the main strategies reported to date for their preparation, including key points such as: cargo loading, surface targeting strategies, purification steps, generation of membrane fragments for the construction of biomimetic materials, preparation of synthetic membranes inspired in EV composition and subsequent surface decoration.

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Nanotechnology-based manipulation of dendritic cells for enhanced immunotherapy strategies

Cited by 138 publications

References 32 publications

Functional RNA Delivery Targeted to Dendritic Cells By Synthetic Nanoparticles

Functional RNA Delivery Targeted to Dendritic Cells By Synthetic Nanoparticles

Multivalent Glycosylation of Fluorescent Gold Nanoclusters Promotes Increased Human Dendritic Cell Targeting via Multiple Endocytic Pathways

Therapeutic biomaterials based on extracellular vesicles: classification of bio‐engineering and mimetic preparation routes

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