Nanotechnology-assisted microfluidic systems for chemical sensing, biosensing, and bioanalysis

Fattahi, Zahra; Hasanzadeh, Mohammad

doi:10.1016/j.trac.2022.116637

Cited by 45 publications

(19 citation statements)

References 300 publications

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“…AuNPs are affected by molecular interactions and are sensitive to the refractive index of the surrounding medium. Therefore, when the antibody is attached to AuNPs, the refractive index will change, which in turn results in the redshift of the peak ( 39 , 59 ). Figure 3B portrays the visual results of LFIA test strips with different antibody concentrations (0, 0.5, 1, 1.5 μg/ml), and ENR standard solutions (0, 1, 10 ng/ml).…”

Section: Resultsmentioning

confidence: 99%

A highly sensitive lateral flow immunoassay for the rapid and on-site detection of enrofloxacin in milk

et al. 2022

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Enrofloxacin (ENR) is a veterinary antibiotic used to treat bacterial infections in livestock. It chiefly persists in foods and dairy products, which in turn pose severe risks to human health. Hence it is very important to detect the ENR in foods and dairy products to safeguard human health. Herein, we attempted to develop a single-step detection lateral flow immunochromatographic assay (LFIA) using gold nanoparticles (AuNPs) for the rapid and on-site detection of ENR in milk samples. An anti-enrofloxacin monoclonal antibody (ENR-Ab) was conjugated with AuNPs for the specific detection of ENR in milk samples. For sensitivity improvement, many optimization steps were conducted on LFIA test strips. The visual limit of detection (vLOD) was found to be 20 ng/ml with a cut-off value of 50 ng/ml in the milk samples. The obtained LOD and cut-off value were within the safety limit guidelines of the Ministry of food and drug safety, South Korea. The test strip showed negligible cross-reactivity with ENR analogs, and other components of antibiotics, this indicates the high specificity of the LFIA test strip towards ENR. The designed test strip showed good reliability. The visual test results can be seen within 10 min without the need for special equipment. Therefore, the test strip can be employed as a potential detection strategy for the qualitative on-site detection of enrofloxacin in milk samples.

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Section: Resultsmentioning

confidence: 99%

A highly sensitive lateral flow immunoassay for the rapid and on-site detection of enrofloxacin in milk

et al. 2022

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“…Such techniques include continuous-flow microreactors and droplet-based microreactors, based on cross-flow, co-flow, and flow-focusing methods, etc. [ 53 , 54 ].…”

Section: Synthetic Magnetic Nanoparticlesmentioning

confidence: 99%

Biosensors and Drug Delivery in Oncotheranostics Using Inorganic Synthetic and Biogenic Magnetic Nanoparticles

et al. 2022

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Magnetic nanocarriers have attracted attention in translational oncology due to their ability to be employed both for tumor diagnostics and therapy. This review summarizes data on applications of synthetic and biogenic magnetic nanoparticles (MNPs) in oncological theranostics and related areas. The basics of both types of MNPs including synthesis approaches, structure, and physicochemical properties are discussed. The properties of synthetic MNPs and biogenic MNPs are compared with regard to their antitumor therapeutic efficiency, diagnostic potential, biocompatibility, and cellular toxicity. The comparative analysis demonstrates that both synthetic and biogenic MNPs could be efficiently used for cancer theranostics, including biosensorics and drug delivery. At the same time, reduced toxicity of biogenic particles was noted, which makes them advantageous for in vivo applications, such as drug delivery, or MRI imaging of tumors. Adaptability to surface modification based on natural biochemical processes is also noted, as well as good compatibility with tumor cells and proliferation in them. Advances in the bionanotechnology field should lead to the implementation of MNPs in clinical trials.

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“…Microfluidic as a known micro total analysis system (abbreviated as μTAS)/lab‐on‐a‐chip has obtained great achievements in the past decade and still remains tremendous promise in the future (Fattahi & Hasanzadeh, 2022 ; Gu et, al .). Microfluidic chips possess the channel dimensions ranging from tens to hundreds of microns.…”

Section: Crispr/cas Systems‐based Sars‐cov‐2 Detectionmentioning

confidence: 99%

“…Microfluidic chips possess the channel dimensions ranging from tens to hundreds of microns. Microfluidic flow as a typically laminar is significantly affected by the surface tension (Fattahi & Hasanzadeh, 2022 ). The usage of microfluidic chip drastically decreases the sample consumption, especially for some pricey, valuable, and small biochemicals.…”

Section: Crispr/cas Systems‐based Sars‐cov‐2 Detectionmentioning

confidence: 99%

“…The usage of microfluidic chip drastically decreases the sample consumption, especially for some pricey, valuable, and small biochemicals. Compared with some standard test methods, the micro amount can significantly improve the reaction rate, efficiently increase the detection throughput, and greatly shorten the test time (Bing Chen, Li, Xu, & Yang, 2022 ; Fattahi & Hasanzadeh, 2022 ; Yahui Wang, Ma, et al., 2021 ). Due to its superior performances (e.g., small size, low reagent/sample consumption, easy integration, and rapid analysis speed), microfluidic chips have been successfully integrated with CRISPR/Cas system‐based biosensors for the rapid and POC diagnosis of SARS‐CoV‐2 (B. Chen et al., 2022 ; F.‐E.…”

Section: Crispr/cas Systems‐based Sars‐cov‐2 Detectionmentioning

confidence: 99%

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Point‐of‐care CRISPR/Cas biosensing technology: A promising tool for preventing the possible COVID‐19 resurgence caused by contaminated cold‐chain food and packaging

et al. 2022

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The ongoing coronavirus disease 2019 (COVID‐19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) has caused great public health concern and has been a global threat due to its high transmissibility and morbidity. Although the SARS‐CoV‐2 transmission mainly relies on the person‐to‐person route through the respiratory droplets, the possible transmission through the contaminated cold‐chain food and packaging to humans has raised widespread concerns. This review discussed the possibility of SARS‐CoV‐2 transmission via the contaminated cold‐chain food and packaging by tracing the occurrence, the survival of SARS‐CoV‐2 in the contaminated cold‐chain food and packaging, as well as the transmission and outbreaks related to the contaminated cold‐chain food and packaging. Rapid, accurate, and reliable diagnostics of SARS‐CoV‐2 is of great importance for preventing and controlling the COVID‐19 resurgence. Therefore, we summarized the recent advances on the emerging clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system‐based biosensing technology that is promising and powerful for preventing the possible COVID‐19 resurgence caused by the contaminated cold‐chain food and packaging during the COVID‐19 pandemic, including CRISPR/Cas system‐based biosensors and their integration with portable devices (e.g., smartphone, lateral flow assays, microfluidic chips, and nanopores). Impressively, this review not only provided an insight on the possibility of SARS‐CoV‐2 transmission through the food supply chain, but also proposed the future opportunities and challenges on the development of CRISPR/Cas system‐based detection methods for the diagnosis of SARS‐CoV‐2.

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Nanotechnology-assisted microfluidic systems for chemical sensing, biosensing, and bioanalysis

Cited by 45 publications

References 300 publications

A highly sensitive lateral flow immunoassay for the rapid and on-site detection of enrofloxacin in milk

A highly sensitive lateral flow immunoassay for the rapid and on-site detection of enrofloxacin in milk

Biosensors and Drug Delivery in Oncotheranostics Using Inorganic Synthetic and Biogenic Magnetic Nanoparticles

Point‐of‐care CRISPR/Cas biosensing technology: A promising tool for preventing the possible COVID‐19 resurgence caused by contaminated cold‐chain food and packaging

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